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T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity

Steven Gillis, Mary M. Ferm, Winny Ou and Kendall A. Smith
J Immunol June 1, 1978, 120 (6) 2027-2032;
Steven Gillis
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Mary M. Ferm
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Winny Ou
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Kendall A. Smith
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Abstract

Several soluble factors have recently been associated with the proliferation and differentiation of thymus-derived lymphocytes. One of these factors present in medium conditioned by T cell mitogen-stimulated lymphocytes has the ability to promote the long-term culture of normal and antigen-specific cytotoxic T cells. We report a method to test for this proliferative stimulus in the form of a sensitive microassay based upon the tritiated thymidine incorporation of continuous murine tumorspecific cytotoxic T cell lines (CTLL). The microassay requires microliter quantities of sample fluid and is amenable to quantitative analysis. This highly reproducible, quantitative assay for T cell growth factor (TCGF) has allowed investigation as to the kinetics of TCGF generation and has revealed that T lymphocytes are required for its production. Further investigation has supported the notion that this nonspecies-specific factor is actively removed from tissue culture medium by the proliferation of either T cell mitogen-activated lymphocytes or CTLL.

Footnotes

  • ↵1 This work was supported in part by Research Grant CA-17643 and Research Contract NO1-CB-74141 from the National Cancer Institute, Bethesda, Maryland, and a research grant from the National Leukemia Association, Inc.

  • Received January 23, 1978.
  • Accepted March 21, 1978.
  • Copyright © 1978 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 120, Issue 6
1 Jun 1978
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T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity
Steven Gillis, Mary M. Ferm, Winny Ou, Kendall A. Smith
The Journal of Immunology June 1, 1978, 120 (6) 2027-2032;

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T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity
Steven Gillis, Mary M. Ferm, Winny Ou, Kendall A. Smith
The Journal of Immunology June 1, 1978, 120 (6) 2027-2032;
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Print ISSN 0022-1767        Online ISSN 1550-6606