Abstract
The occurrence of species-specific and strain-specific antigens in three strains of Mycoplasma arginini (G-230, leonis and 23243) was studied by two-dimensional immunoelectrophoresis. Approximately 20 antigenic components could be detected in each strain. It was possible to analyze 6 to 7 major and distinct components from each strain by two techniques: “enhancement” where antigen to an additional strain is added to the first phase of the electrophoresis which increases the size of common peaks and “suppression” where antiserum to an additional strain is incorporated in the second phase whereby peak size of components to which both sera have antibody are decreased. A total of 10 distinct antigens were recognized. Electrophoretic mobilities relative to bovine albumin ranged from 0.2 to 1.08. Three components were common to all strains; two of these represented major amounts of material. Four components represented strain-specific components. Unique fast components were found both in strains 23243 and G-230. Three antigens were distributed into only two of the three strains. The electrophoretic mobilities of some common antigens were quite different between strains.
Footnotes
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↵1 The work was supported by Research Grants AI-06720 and AI-12005 and Training Grant AI206 from the United States Public Health Service and was supported in part by the United States Army Research and Development Command, Department of the Army under Research Contract DADA 17-72-C-2069.
- Received October 28, 1974.
- Copyright © 1975 by The American Association of Immunologists, Inc.
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