Abstract
Lymph node cells (LNC) or spleen cells from normal BALB/c mice were sensitized in vitro by co-cultivation with mitomycin C-treated allogeneic sarcoma cells, and their reactivity measured with a microcytotoxicity assay. Following 5 days of sensitization, a specific cytotoxic effect against tumor cells carrying the sensitizing alloantigens was observed; this is in agreement with previously published findings. Lymphoid cells tested after 3 days of sensitization, however, specifically increased the number of target cells remaining in the Microtest plates at the conclusion of the microcytotoxicity assays.
LNC from normal BALB/c mice were also sensitized in vitro against tumor specific antigens using four different methylcholanthrene-induced sarcomas of BALB/c origin. After 6 days of sensitization the LNC were specifically cytotoxic for the immunizing tumor cell line. After 3 days of sensitization, however, the LNC gave a specific increase of the number of remaining target tumor cells.
Footnotes
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↵2 This investigator was supported in part as a Graduate Research Trainee (Ca05040), National Cancer Institute.
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↵1 This investigation was supported by Grants CA 10188 and CA 10189 from the National Institutes of Health, by Grant 1C-56D from the American Cancer Society, by Contract NIH-NCI-71-2171 within the Virus Cancer Program of the National Cancer Institute, and by Contract NO1 CB 23887 from the National Cancer Institute.
- Received December 6, 1974.
- Copyright © 1975 by The American Association of Immunologists, Inc.
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