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Specific Purification of Lymphocyte Populations on a Digestible Immunoabsorbent

Stuart F. Schlossman and Leslie Hudson
J Immunol January 1, 1973, 110 (1) 313-315;
Stuart F. Schlossman
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Leslie Hudson
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Summary

A new method utilizing an enzyme digestible immunoabsorbent has permitted fractionation and purification of lymphoid cells according to their surface properties. Spleen cells filtered through Sephadex anti-Fab columns were specifically depleted of immunoglobulin-bearing B cells. Passed cells, on the other hand, were enriched both in T and null staining cells. Ninety-eight per cent of the absorbed B cells could be recovered in a functionally viable state after solubilization of the immunoabsorbent with dextranase.

Footnotes

  • ↵1 This work was supported by grants from the Medical Research Council, England.

  • Copyright, 1972, by The Williams & Wilkins Company
  • Copyright © 1973 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 110, Issue 1
1 Jan 1973
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Specific Purification of Lymphocyte Populations on a Digestible Immunoabsorbent
Stuart F. Schlossman, Leslie Hudson
The Journal of Immunology January 1, 1973, 110 (1) 313-315;

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Specific Purification of Lymphocyte Populations on a Digestible Immunoabsorbent
Stuart F. Schlossman, Leslie Hudson
The Journal of Immunology January 1, 1973, 110 (1) 313-315;
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Print ISSN 0022-1767        Online ISSN 1550-6606