Abstract
Precipitin antigen was prepared from cultures infected with V-Z virus. Five separate antigens were identified. Reactions with radiolabeled antigen were detected by autoradiography. Partial purification of antigens could be accomplished in a discontinuous sucrose gradient, yet too little antigen was recovered from DEAE to make this a practicable purification method. Most of the antigenic activity was eluted from G-200 Sephadex. Antigens were identified by their degree of chemical stability. The α antigen was the only one which was stable at pH 3. The β antigen was destroyed by CsCl or DOC. DOC released the γ Δ, and ε antigens from the infectious viral particle.
Footnotes
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↵1 This work was supported by Grant No. AI-06880 of the National Institutes of Health, Grant No. CC00465 of the Center for Disease Control and a grant from the World Health Organization.
- Received September 3, 1971.
- Copyright © 1972 by The American Association of Immunologists, Inc.
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