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The Antigens of Varicella-Zoster Virus

Philip A. Brunell, Miriam Granat and Anne A. Gershon
J Immunol March 1, 1972, 108 (3) 731-737;
Philip A. Brunell
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Miriam Granat
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Anne A. Gershon
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Abstract

Precipitin antigen was prepared from cultures infected with V-Z virus. Five separate antigens were identified. Reactions with radiolabeled antigen were detected by autoradiography. Partial purification of antigens could be accomplished in a discontinuous sucrose gradient, yet too little antigen was recovered from DEAE to make this a practicable purification method. Most of the antigenic activity was eluted from G-200 Sephadex. Antigens were identified by their degree of chemical stability. The α antigen was the only one which was stable at pH 3. The β antigen was destroyed by CsCl or DOC. DOC released the γ Δ, and ε antigens from the infectious viral particle.

Footnotes

  • ↵1 This work was supported by Grant No. AI-06880 of the National Institutes of Health, Grant No. CC00465 of the Center for Disease Control and a grant from the World Health Organization.

  • Received September 3, 1971.
  • Copyright © 1972 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 108, Issue 3
1 Mar 1972
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The Antigens of Varicella-Zoster Virus
Philip A. Brunell, Miriam Granat, Anne A. Gershon
The Journal of Immunology March 1, 1972, 108 (3) 731-737;

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The Antigens of Varicella-Zoster Virus
Philip A. Brunell, Miriam Granat, Anne A. Gershon
The Journal of Immunology March 1, 1972, 108 (3) 731-737;
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Print ISSN 0022-1767        Online ISSN 1550-6606