Abstract
RNA extracted from the spleens of Swiss Webster mice immunized with bacteriophage 81a, which infects Streptococcus faecalis var. zymogenes, induced the formation of specific phage-neutralizing antibody when added to nonimmune mouse spleen fragments in culture. Antibody formation could be elicited by the addition of immunogenic RNA derived from one inbred mouse strain to nonimmune spleen fragments from other inbred mouse strains. The activity of immunogenic RNA was inhibited by treatment with RNase, but not by DNase, trypsin, amylase or moderate amounts of pronase. The active RNA sedimented in the lightest fraction of a 5% to 20% linear sucrose gradient, and was estimated to have an average sedimentation coefficient of 8.2 S by analytical ultracentrifugation.
Footnotes
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↵2 Recipient of United States Public Health Service Predoctoral Award 1-F1-GM-38, 593-01.
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↵1 This work was supported by United States Public Health Service Grant AI-07137 and was presented in part at the 52nd Annual Meeting of the American Association of Immunologists in April, 1968, at Atlantic City, New Jersey.
- Received June 16, 1969.
- Copyright, 1970, by The Williams & Wilkins Company
- Copyright © 1970 by The American Association of Immunologists, Inc.
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