Table II. Summary of isotypic distribution and MPER reactivities in primary screen of hybridoma clones derived from 2F5 and 4E10 complete KI spleens
Fusion Identification no.MiceTotal Seeded WellsWells with Cell GrowthaIgM+bIgG+IgA+MPER+c
2F5-V1012F5VH+/+ × VL+/+304051022500224
4E10-V24E10VH+/+ × VL+/+3200146500043
4E10-V34E10VH+/+ × VL+/+160017410000100
  • All fusions were performed using NS0-bcl2 myeloma fusion partner lines and represent a different individual mouse.

  • a Culture plates were screened under microscope for cell growth.

  • b Ig levels /isotypes of all cloned hybridoma lines were determined by ELISA as described in Materials and Methods. Lines were considered positive if >3× above background OD binding was detected in 1:40 diluted supernatants.

  • c MPER reactivity of 2F5 or 4E10 hybridoma lines was determined by ELISA to their nominal MPER epitopes (as specified by SP62 and MPR.03 peptides, respectively). Lines were considered positive if >3× above background OD binding was detected in 1:2 diluted supernatants.