Table I.

Buffer protocols

StepVolumeDescriptionParameters
10.00Isocratic flowBuffer A 100% Buffer B 0%
21.00Zero BaselineUV Detector
31.00Load/inject sample
43.00Isocratic flowBuffer A 100% Buffer B 0%
55.00Isocratic flowBuffer A 60% Buffer B 40%
68.00Isocratic flowBuffer A 0% Buffer B 100%