Table I.

Oligonucleotide sequences of spectratyping primers

Vβ and Cβ PrimersSequence 5′–3′Size of Primer (bp)Distance to CDR3a (bp)
Vβ1CT GAA TGC CCA GAC AGC TCC AAG C2483
Vβ5.1CAT TAT GAT AAA ATG GAG AGA GAT23135
Vβ8.1CAT TAC TCA TAT GTC GCT GAC21141
Vβ8.3T GCT GGC AAC CTT CGA ATA GGA22127
Vβ9TCT CTC TAC ATT GGC TCT GCA GGC2457
Vβ10ATC AAG TCT GTA GAG CCG GAG GA2348
Vβ11G CAC TCA ACT CTG AAG ATC CAG AGC2564
Vβ12G ATG GTG GGG CTT TCA AGG ATC22117
Vβ16C ACT CTG AAA ATC CAA CCC AC2158
CTT GGG TGG AGT CAC ATT TCT2260
  • a The length in bp for V primers is counted from the first base in the V gene corresponding to the 5′ end of the primer through to the last base of the V gene before the CDR3 region. The length in bp for the C primer is counted from the first base of the J gene after the CDR3 region through to the last base in the C gene corresponding to the 5′ end of the primer (42 ).