Table I.

Tumors of hematopoietic and nonhematopoietic origin transduced with FPPS-specific shRNA AS22 activate Vγ9Vδ2 T cellsa

Stimulator cellsExpressed shRNA% CD107a Positive in Vδ2 T cells
Medium0.1 μM BrHPP
AsPC-1AS3.717.6
AS229.128.2
HepG2AS7.376.2
AS2253.179.5
293TAS4.963.2
AS2218.063.2
RajiAS5.885.3
AS2242.685.7
DaudiNone26.685.3
NoneNone3.66.8
  • a Cells (3 × 104) of a Vγ9Vδ2 T cell line were incubated for 4 h with 105 cells of the indicated cell lines in the presence of PE-anti-CD107a Ab. Thereafter cells were harvested and costained with FITC-anti-Vδ2 TCR Ab.