Table I.

The effect of CCR3 chemokine neutralizing Abs on HPAEC-CM-induced eosinophil survivala

No AbPlus Neutralizing Ab
Ab DetailsControlGM-CSFHPAEC-CMIgGHPAEC-CM + IgGAb aloneHPAEC-CMGM-CSF
Anti-CCL1110038 ± 433 ± 395 ± 2341 ± 1NS100 ± 16105 ± 12b38 ± 4
Anti-CCL2410039 ± 541 ± 275 ± 1359 ± 6NS98 ± 472 ± 20NS40 ± 15
Anti-CCL2610037 ± 158 ± 9109 ± 2465 ± 5NS105 ± 1089 ± 15NS62 ± 27
Anti-CCL510035 ± 050 ± 884 ± 1265 ± 2NS100 ± 987 ± 10NS53 ± 8
Anti-CCL2710028 ± 239 ± 476 ± 044 ± 3NS69 ± 9.542 ± 2NS41 ± 5
  • a Cell medium was preincubated with the Ab or isotype-matched IgG control for 1 h at 37° C before resuspension with eosinophils as described in Materials and Methods. Eosinophils were harvested at 24 h, and apoptosis was assessed morphologically. Values represent apoptosis expressed as a percentage of the control value and are the mean ± SEM of three independent experiments (

  • b , p < 0.05 compared with HPAEC-CM values). Note that each set of neutralization experiments was conducted separately due to limitations on human eosinophil number.