Table II. Genes required for functional competencies that are acquired during maturation
GeneNotesRefs.
Cell Division Competence in Response to CD3/CD28 (M1)
TAK1Decreased CD4 SP thymocyte proliferation (CD4-Cre cKO)(12)
Decreased thymocyte proliferation (Lck-Cre cKO)(10, 52)
c-FLIPDecreased splenic T cell proliferation (KO/Rag KO chimeras)(54)
Decreased thymocyte proliferation (Lck-Cre cKO)(21)
Ubc13Decreased splenic T cell proliferation (Lck-Cre KO)(16)
RelBDecreased thymocyte proliferation in response to anti-CD3 (KO)(14)
IKK2Decreased thymocyte proliferation to anti-CD3 in IKK2/TNFR1 KO(13)
ZFATDecreased splenic CD4 T cell proliferation (CD4-Cre cKO)(48)
Casp8Decreased splenic CD4 T cell proliferation (Lck-Cre cKO)(55)
Bclaf1Decreased splenic T cell proliferation (KO/Rag2 KO chimeras)(56)
Protection from TCR and Death Receptor Signals (M1)
c-FLIPIncreased thymocyte and peripheral T cell apoptosis in response to TCR, TNF and Fas signals (Lck-Cre cKO, ER-Cre cKO)(20, 21)
TAK1Increased thymocyte apoptosis in response to TNF, CD3 (Lck-Cre cKO)(10, 53)
NEMOIncreased thymocyte apoptosis at steady-state (CD4-Cre)(15)
IKK2Increased thymocyte apoptosis to TNF, Fas (KO fetal liver stem cell transplant), partial rescue of thymocyte numbers in IKK2/TNFR1 KO(13)
RIP1Increased thymocyte apoptosis in response to Fas, TNF (KO)(19)
RelBIncreased thymocyte apoptosis in response to anti-CD3 (KO)(14)
Ubc13Regulates Tak1 and NF-κB signaling in thymocytes (Lck-Cre cKO)(16)
Gpx4Increased ferroptosis in splenic T cells to superantigen or anti-CD3 (CD4-Cre)(51)
Egress (M2)
S1P1Impaired egress in deficient thymocytes (KO fetal liver chimeras)(25)
KLF2Impaired egress in deficient thymocytes with reduced S1P1 (KO fetal liver chimeras, CD4-Cre)(24)
PAK2Impaired egress associated with reduced KLF2/S1P1 (CD4-Cre)(29)
Mst1/2Impaired egress with normal KLF2/S1P1 expression (Lck-Cre)(28)
mDia-1Impaired egress in deficient thymocytes (KO)(27)
Coro1Impaired egress with normal S1P1 expression (point mutant)(26)
Foxo1Decreased expression of KLF2 (CD4-Cre cKO), regulates thymic egress with Foxo3 (unpublished observations)(30)
Paxip1Decreased S1P1, but normal KLF2, Foxo1 expression (KO)(32)
IL-2 Production to CD3/CD28 Stimulation (Begins at M1 and Is Optimal at MNT)
NKAPDecreased IL-2 production by splenic CD4+ RTE (CD4-Cre cKO)(38)
ZFATDecreased IL-2 production by splenic CD4 T cells (CD4-Cre cKO)(48)
Casp8Decreased IL-2 production by splenic T cells (Lck-Cre cKO)(55)
TAK1Decreased IL-2 production in CD4 SP thymocytes (CD4-Cre cKO)(12)
c-FLIPDecreased IL-2 production in thymocytes (Lck-Cre cKO)(20)
TNF Licensing to CD3/CD28 Stimulation (Begins at M2/RTE and Is Optimal at MNT)
HDAC3Defect in TNF production in CD4 and CD8 RTE and MNT (CD4-Cre cKO)(42)
NKAPDefect in TNF production in CD4 and CD8 RTE and MNT (CD4-Cre cKO)Hsu and Shapiro, unpublished observations
MNT Survival or Maintenance (Peripheral T Cell Lymphopenia)
NKAPPeripheral T cells eliminated by complement (CD4-Cre cKO)(50)
Zfp335Few peripheral T cells, not rescued by a Bcl-2 transgene (point mutant)(57)
HDAC3Peripheral T cells eliminated by complement (CD4-Cre cKO)(42)
TAK1Decreased response to IL-7 but normal IL-7Rα expression (CD4-Cre cKO)(12)
ZFATDecreased survival, IL-7Rα and Bcl-2 expression (CD4-Cre cKO)(48)
Foxo1Decreased survival, IL-7Rα and Bcl-2 expression (CD4-Cre cKO)(30)
IKK2Decreased survival, IL-7Rα but not Bcl-2 expression (CD4-Cre cKO)(46, 47)
Other: Cause for Defects Largely Unknown
FLVCRNormal thymic populations but few peripheral T cells (CD4-Cre cKO)(58)
Casp8Normal thymic populations but few peripheral T cells (Lck-Cre cKO)(55)
BptfLoss of mature SP thymocytes, decreased IL-7Rα expression but not rescued by Bcl-2 transgene (Lck-Cre cKO)(49)