Table I.

Mass spectrometry identification of proteins in 27-kDa fraction from M. tuberculosis H37Rv electroelution

ProteinPeptide SequenceaObserved Mass-to-Charge RatioChargePredicted MH+Peptide AA Positionb
LprAcLEGDISNTPQTVATGSATLLVGNK1194.222387.6357–80
TVPTTVWIASDGSSHLVQIQIAPTK1325.622651.01175–199
LTSEDVK791.41791.87168–174
VTKLEGDISNTPQTVATGSATLLVGNK1358.222716.0454–80
GLANLLANLK1027.611027.24124–133
DASVAGSQQADGVATTK803.921606.67134–150
LprGdGSGNSVQMTLSK613.121209.36186–197
IPGLSLK727.71727.9242–48
DTINGQNTIR566.721132.21132–141
SAHM#VLTVNGK587.421173.3731–41
  • a Peptides generated and identified following trypsin digestion of fraction contents. #, Methionine oxidation.

  • b Amino acid position in mature lipoproteins.

  • c Coverage of 35.25% mature LprA was identified by peptide sequence.

  • d Coverage of 19.05% mature LprG was identified by peptide sequence.