Table I. Cell apoptosis and necrosis after UV radiation
Cell TypeUV Dose (mJ/cm2)Post-UV (h)Apoptosis (%; Mean ± SD)Necrosis (%; Mean ± SD)
HT-2915003.2 ± 1.02.3 ± 0.9
211.8 ± 4.12.0 ± 1.0
460.7 ± 7.3***5.9 ± 2.0
687.6 ± 6.2***16.2 ± 3.0**
8100***28.1 ± 5.8***
18100***100***
T47D15002.4 ± 0.91.9 ± 0.5
689.6 ± 6.2***18.2 ± 6.6**
MCF712501.9 ± 1.12.7 ± 0.8
691.3 ± 8.4***20.3 ± 7.5**
MDA23115003.0 ± 0.82.6 ± 0.8
692.8 ± 5.9***17.9 ± 3.2**
MDA43518002.9 ± 1.22.5 ± 0.7
693.4 ± 9.1***21.3 ± 6.0**
HS578T15002.2 ± 0.63.4 ± 0.9
687.3 ± 5.8***17.1 ± 4.6**
MDA46810003.0 ± 0.92.8 ± 0.5
688.6 ± 9.8***15.3 ± 7.8**
HFF-118002.1 ± 0.81.7 ± 0.6
671.4 ± 5.8***14.6 ± 4.8**
HMEC-115003.8 ± 0.62.1 ± 0.6
679.3 ± 6.7***20.6 ± 5.3**
  • Healthy cultured cells (≥95% viability) of various types were irradiated with UV light to induce apoptosis. At different time points post–UV radiation, cells were stained with YO-PRO-1 (green) and PI (red) to determine apoptosis and necrosis, respectively.

  • The data represent one of three independent experiments in which the percentage of apoptotic cells (only green) and necrotic cells (green and red double staining [orange]) was determined microscopically in 10 fields of view against the total number of cells.

  • **p ≤ 0.01, ***p ≤ 0.001.