Table I.

Temporal mRNA expression levels of select cytokines and chemokines in ankles of B. burgdorferi-infected micea

Day PostinfectionFold Change in mRNA Expression Levelb
KCMCP-1MIP-2MIP-1αIL-6IFN-γ
B6C3HB6C3HB6C3HB6C3HB6C3HB6C3H
01.01.01.01.01.01.01.01.01.01.01.01.0
0.32.12.02.61.90.60.90.60.81.00.70.60.6
11.31.53.84.30.60.80.90.60.60.60.60.6
31.96.80.84.20.71.30.60.30.10.11.70.6
50.62.86.21.31.63.61.50.40.10.11.70.1
71.59.43.45.21.00.84.20.80.20.10.60.1
103.229.58.819.00.75.20.81.50.20.12.80.2
142.115.83.62.40.511.00.32.80.10.50.21.9
213.515.610.11.85.215.41.83.40.40.712.78.9
  • a Tibiotarsal joints from B. burgdorferi-infected mice were collected at the times indicated and total RNA was extracted. Levels of mRNA for each cytokine or chemokine were determined using quantitative real-time PCR as described in Materials and Methods.

  • b The levels of mRNA were expressed as a fold change in the expression level of untreated normal mice (time = 0), normalized to murine Nidogen gene expression (n = 4). The experiment was conducted twice and the mean of two independent experiments is shown.