Table I. Comparison of T cell subsets in control and CD4-Cre/ShcFFF mice
ControlCSDO11.10OT-IIH-YOT-IdLckF505
ControlCSControlCSControlCSControlCSControlCS
Total thymocytes191 ± 67119 ± 35143 ± 27101 ± 33106 ± 1649 ± 2495 ± 467 ± 15117 ± 2560 ± 12220 ± 27157 ± 36
 DN6.3 ± 2.65.8 ± 2.224 ± 1123 ± 717 ± 612 ± 519 ± 217 ± 615 ± 3.510 ± 27 ± 16.6 ± 2
 DP146 ± 47.990 ± 24.874 ± 2346 ± 2061 ± 627 ± 1445 ± 334 ± 557 ± 1220 ± 4108 ± 2197 ± 30
 CD4 SP16.9 ± 67.8 ± 2.742 ± 1223 ± 830 ± 411 ± 5110 ± 1052 ± 12
 CD8 SP5.8 ± 2.13.3 ± 1.423 ± 612 ± 319 ± 310 ± 1.56.2 ± 1.85.5 ± 1.2
Total splenocytes84 ± 11.772 ± 17.587.5 ± 1669 ± 1759 ± 2a54 ± 6a71 ± 1859 ± 1287 ± 21100 ± 2988 ± 1370 ± 10
 CD4 T cells8.9 ± 2.43.2 ± 1.88.5 ± 1.66 ± 25 ± 0.24 ± 0.412 ± 1.17.7 ± 1
 CD8 T cells5 ± 1.81.7 ± 1.75 ± 1.24.4 ± 23.6 ± 1.81 ± 0.82.1 ± 0.11.9 ± 0.5
  • Thymi and spleen from mice of the indicated genotypes were analyzed by flow cytometry, and numbers were determined based on their fractions within the total population. For the transgenic TCR setting, the cells were gated first on cells expressing the transgenic TCR before estimating the specific subsets. Data shown are absolute numbers (mean ± SD) of T cell subsets.

  • a n = 4 mice analyzed.

  • —, subsets that do not express the indicated transgenic TCR and were not included in the analysis; CS, CD4-Cre/ShcFFF–transgenic mice.