Table II.

Rate of tolerance of purified näive clone-4 TCR CD8+ T cells adoptively transferred into InsHA+/− micea

Time of Immunization After TransferIncidence of Diabetes (%)
No. of clone-4 cells transferred
0 days100100100
5 days100ND0
2 wkND1000
1 mo10000
  • a Groups of four adult InsHA+/− mice were injected i.v. with various numbers of purified clone-4 TCR CD8+ T cells as indicated. At various time points following transfer, mice were immunized with 1200 HA U of influenza virus A/PR/8, and blood glucose levels were monitored every 3 days. Mice were considered diabetic when their blood glucose level exceeded 300 mg/dl. ND, not determined.