Table I.

Circulating blood cell numbers and composition before and after TG challenge in CD31KO micea

Total WBC (×103/mm3)% Lymphocytes% Monocytes% Neutrophils\E
PBL PRE-TG (n = 17)
WT11.83 ± 1.4366.03 ± 3.188.34 ± 0.8313.04 ± 1.2714.26 ± 2.32b
CD31KO11.78 ± 1.1671.68 ± 2.028.16 ± 0.459.80 ± 1.237.96 ± 1.16b c
PBL POST-TG (n = 12)\E
WT6.89 ± 0.7420.37 ± 1.869.71 ± 0.9259.80 ± 3.2559.63 ± 3.00b
CD31KO6.15 ± 0.6030.10 ± 2.04**12.67 ± 1.0045.85 ± 2.92**46.30 ± 2.90b **
  • a Total WBC numbers and percentages of circulating leukocytes before (PRE-TG) and 4 h after (POST-TG) i.p. injection with 1.5 ml 4% TG. Cell numbers were determined by direct counting using an hemocytometer. Percentages are calculated from forward/side scatter profiles obtained by FACS analysis of blood from tail vein.

  • b Percent neutrophils was also calculated from percent Gr-1+ cells in Mac-1/Gr-1 double-stained samples.

  • c p < 0.05, ** p < 0.005 for CD31KO vs WT, (Student’s t test). All other comparisons not statistically significant (p > 0.05).