Table II.

Effect of various mite and non-mite proteases on cytokine release from the human bronchial epithelial cell line BEAS-2B

Protease (100 ng/ml)GM-CSFCytokine (mean pg (SEM)/106 BEAS-2B cells)
(p <)aIL-6(p <)IL-8(p <)
None (medium alone)105 (19)108 (28)2,980 (450)
Der p 1712 (95)0.0011,108 (289)0.0218,900 (3,600)0.01
Der p 9890 (135)0.0011,794 (324)0.00117,500 (2,330)0.001
Thrombin734 (82)0.0011,626 (297)0.00112,900 (2,146)0.01
Trypsin112 (34)NS986 (145)0.0015,600 (780)0.05
  • a Differences in mean values for individual proteases compared with those obtained with medium alone were determined using the Student t test (n = 6).