Table I.

Binding affinities of Ac1–9 analogue peptides for I-Aua

PeptideIC50 (nM)Relative Binding Affinity
Ac1–9(4K)>100,000<0.00001
Ac1–9(4Y)5671
Ac1–9(4Y,6C)1,6880.333
Ac1–9(4Y,6G)3,6800.15
Ac1–9(4Y,6T)3,7500.15
Ac1–9(4Y,6V)2,9580.19
OVA323–3391,1360.5
  • a The I-Au-binding affinities of Ac1–9, Ac1–9(4Y), and position 6 APL, previously identified as antagonists of the Tg4 TCL, were compared using a competitive binding assay. As controls, the null Cys6 APL and the OVA323–339 peptide were also tested.