Table I.

Thymocyte and spleen cell populations in 3A9 TCR wild-type, Fas-deficient, and Bcl-2 transgenic micea

ThymusSpleen
TotalCD4+CD8+ CellsTotal CD4+ CellsVβ8+CD4+ CellsTotal CD8+ CellsVβ8+CD8+ CellsTotalVβ8+CD4+ CellsVβ8+CD8+ Cells
3A9/+93.4 ± 15.050.4 ± 12.332.2 ± 6.229.9 ± 7.24.1 ± 0.61.8 ± 0.986.1 ± 3.812.1 ± 3.47.7 ± 1.3
3A9/lpr89.3 ± 12.846.4 ± 11.934.9 ± 8.932.4 ± 4.83.4 ± 1.01.8 ± 0.497.7 ± 5.112.6 ± 2.69.7 ± 3.7
3A9/Bcl-2215.0 ± 38.268.8 ± 9.383.9 ± 14.576.2 ± 12.146.7 ± 10.444.0 ± 9.2127.6 ± 29.641.9 ± 8.318.5 ± 4.4
  • a Thymi and spleens were harvested from 6- to 10-wk-old wild-type (3A9/+, n = 12), lpr (3A9/lpr, n = 12), and Bcl-2 transgenic (3A9/Bcl-2, n = 12) mice that expressed the 3A9 TCR transgene alone (3A9) or in conjunction with a transgene for HEL protein (3A9 × HEL). The total cell counts obtained were multiplied by the frequencies of the relevant cell populations, as determined by FACS, in order to obtain cell counts for individual cell populations. All values are × 10−6, and the error represents the SD of the mean obtained by averaging the results for all mice analyzed.