Table II. Passive EAE in TLR-deficient C57BL/6 mice and C57BL/6 WT control mice
Experiment No.StrainnIncidence (%)Onset (d)Mean Maximum ScoreCDI
Female
 1C57BL/6510017.0 ± 2.32.8 ± 0.234.4 ± 4.7
C57BL/6.Tlr2−/−30na0.0 ± 0.0*0.0 ± 0.0*
C57BL/6.Tlr9−/−58013.8 ± 1.81.6 ± 0.4*25.1 ± 9.3
 2C57BL/6610023.8 ± 0.52.7 ± 0.221.8 ± 2.1
C57BL/6.Tlr2−/−40na0.0 ± 0.0**0.0 ± 0.0**
C57BL/6.Tlr9−/−410023.3 ± 1.31.5 ± 0.2**4.4 ± 1.5**
 3C57BL/6410019.5 ± 1.82.5 ± 0.533.4 ± 7.9
C57BL/6.Tlr2−/−60na0.0 ± 0.0**0.0 ± 0.0**
C57BL/6.Tlr9−/−510019.4 ± 2.82.1 ± 0.526.0 ± 11.1
 CombinedC57BL/61510020.4 ± 1.22.7 ± 0.229.1 ± 3.0
C57BL/6.Tlr2−/−130na0.0 ± 0.0***0.0 ± 0.0***
C57BL/6.Tlr9−/−1492.818.8 ± 0.51.8 ± 0.2**19.5 ± 5.5
Male
 1C57BL/6410023 ± 1.02.6 ± 0.416.9 ± 3.2
C57BL/6.Tlr2−/−30na0.0 ± 0.00.0 ± 0.0
 2C57BL/6610021 ± 0.23.0 ± 0.022.8 ± 2.4
C57BL/6.Tlr2−/−60na0.0 ± 0.0**0.0 ± 0.0**
 CombinedC57BL/61010021.8 ± 0.52.8 ± 0.118.9 ± 1.9
C57BL/6.Tlr2−/−90na0.0 ± 0.0***0.0 ± 0.0***
  • Passive EAE was induced by adoptive transfer of in vitro-activated lymph node and splenic T cells from MOG/CFA-immunized C57BL/6 mice into C57BL/6.Tlr2−/−, C57BL/6.Tlr9−/−, and C57BL/6 control WT mice. Statistical differences between recipients of cells transferred from TLR-deficient C57BL/6 and C57BL/6 WT control mice are indicated.

  • *p < 0.05, **p < 0.01, ***p < 0.001, uncorrected Mann–Whitney U test.

  • na, not appropriate.