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On the Cover: Eotaxin (CCL11) promotes angiogenesis in the chick chorioallantoic membrane (CAM) assay. Plastic coverslips containing 10 (B) or 100 (C) ng of CCL11 were placed on the chorioallantoic membrane of 10-day-old embryos. Distilled water, which was used as a solvent, served as the negative control (A). The percentage of positive CAMs for each CCL11 concentration was scored. bFGF (10 ng) was used as a positive control (D-not included). E and F, Eosinophils and heterophil infiltrates observed by Giemsa staining of CAM histological sections, control (E), and CCL11 at 100 ng (F). Twenty embryonated eggs were used for each data point, and the experiment was repeated twice. A representative experiment is shown. (Modified from Figure 3 from Salcedo, R., H. A. Young, M. L. Ponce, J. M. Ward, H. K. Kleinman, W. J. Kleinman, W. J. Murphy, and J. J. Oppenheim. Eotaxin (CCL11) induces in vivo angiogenic responses by human CCR3+ endothelial cells J. Immunol.166:7571.)
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