Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Annual Meeting Abstracts
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • Rights and Permissions
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Annual Meeting Abstracts
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • Rights and Permissions
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

Aeroallergens Induce Reactive Oxygen Species Production and DNA Damage and Dampen Antioxidant Responses in Bronchial Epithelial Cells

Tze Khee Chan, W. S. Daniel Tan, Hong Yong Peh and W. S. Fred Wong
J Immunol May 19, 2017, 1600657; DOI: https://doi.org/10.4049/jimmunol.1600657
Tze Khee Chan
Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 117600, Singapore;Immunology Program, Life Science Institute, National University of Singapore, Singapore 117456, Singapore;Infectious Diseases Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Campus for Research Excellence and Technological Enterprise, Singapore 138602, Singapore; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. S. Daniel Tan
Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 117600, Singapore;Immunology Program, Life Science Institute, National University of Singapore, Singapore 117456, Singapore;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for W. S. Daniel Tan
Hong Yong Peh
Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 117600, Singapore;Immunology Program, Life Science Institute, National University of Singapore, Singapore 117456, Singapore;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. S. Fred Wong
Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 117600, Singapore;Immunology Program, Life Science Institute, National University of Singapore, Singapore 117456, Singapore;Molecular Mechanisms of Inflammatory Diseases Interdisciplinary Research Group, Singapore-HUJ Alliance for Research and Enterprise, Campus for Research Excellence and Technological Enterprise, Singapore 138602, Singapore
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF
Loading

Abstract

Exposure to environmental allergens is a major risk factor for asthma development. Allergens possess proteolytic activity that is capable of disrupting the airway epithelium. Although there is increasing evidence pointing to asthma as an epithelial disease, the underlying mechanism that drives asthma has not been fully elucidated. In this study, we investigated the direct DNA damage potential of aeroallergens on human bronchial epithelial cells and elucidated the mechanisms mediating the damage. Human bronchial epithelial cells, BEAS-2B, directly exposed to house dust mites (HDM) resulted in enhanced DNA damage, as measured by the CometChip and the staining of DNA double-strand break marker, γH2AX. HDM stimulated cellular reactive oxygen species production, increased mitochondrial oxidative stress, and promoted nitrosative stress. Notably, expression of nuclear factor erythroid 2–related factor 2–dependent antioxidant genes was reduced immediately after HDM exposure, suggesting that HDM altered antioxidant responses. HDM exposure also reduced cell proliferation and induced cell death. Importantly, HDM-induced DNA damage can be prevented by the antioxidants glutathione and catalase, suggesting that HDM-induced reactive oxygen and nitrogen species can be neutralized by antioxidants. Mechanistic studies revealed that HDM-induced cellular injury is NADPH oxidase (NOX)-dependent, and apocynin, a NOX inhibitor, protected cells from double-strand breaks induced by HDM. Our results show that direct exposure of bronchial epithelial cells to HDM leads to the production of reactive oxygen and nitrogen species that damage DNA and induce cytotoxicity. Antioxidants and NOX inhibitors can prevent HDM-induced DNA damage, revealing a novel role for antioxidants and NOX inhibitors in mitigating allergic airway disease.

Footnotes

  • This work was supported by the National Medical Research Council (Grant NMRC/CBRG/0027/2012) and the National University Health System (Seed Grant R-184-000-238-112). This study was also supported by the National Research Foundation Singapore through the Singapore-MIT Alliance for Research and Technology program, as well as Campus for Research Excellence and Technological Enterprise Grant R-184-000-269-592.

  • The online version of this article contains supplemental material.

  • Received April 14, 2016.
  • Accepted April 21, 2017.
  • Copyright © 2017 by The American Association of Immunologists, Inc.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Log in using your username and password

Forgot your user name or password?
Next
Back to top

In this issue

The Journal of Immunology: 200 (9)
The Journal of Immunology
Vol. 200, Issue 9
1 May 2018
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Advertising (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Aeroallergens Induce Reactive Oxygen Species Production and DNA Damage and Dampen Antioxidant Responses in Bronchial Epithelial Cells
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
Citation Tools
Aeroallergens Induce Reactive Oxygen Species Production and DNA Damage and Dampen Antioxidant Responses in Bronchial Epithelial Cells
Tze Khee Chan, W. S. Daniel Tan, Hong Yong Peh, W. S. Fred Wong
The Journal of Immunology May 19, 2017, 1600657; DOI: 10.4049/jimmunol.1600657

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Aeroallergens Induce Reactive Oxygen Species Production and DNA Damage and Dampen Antioxidant Responses in Bronchial Epithelial Cells
Tze Khee Chan, W. S. Daniel Tan, Hong Yong Peh, W. S. Fred Wong
The Journal of Immunology May 19, 2017, 1600657; DOI: 10.4049/jimmunol.1600657
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Elevated Expression of IL-33 and TSLP in the Airways of Human Asthmatics In Vivo: A Potential Biomarker of Severe Refractory Disease
  • Development of Asthma in Inner-City Children: Possible Roles of MAIT Cells and Variation in the Home Environment
  • Biased Generation and In Situ Activation of Lung Tissue–Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma
Show more ALLERGY AND OTHER HYPERSENSITIVITIES

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2018 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606