Summary
Highly purified M proteins have excellent antigenic capacities in rabbits when administered in the form of repository subcutaneous doses with adjuvants. Both mineral oil and peanut oil emulsions prepared with Arlacel A and as little as 10 µg of M protein, after two or three injections, stimulated type-specific protective antibodies. Small doses of M proteins adsorbed to aluminum hydroxide were equally efficacious preparations for vaccine purposes. The attributes of these immunization procedures are discussed in terms of a practical antistreptococcal vaccine for human use. The globulin nature of the antibodies induced in rabbits immunized with purified M proteins or whole bacterial cells was also investigated. Both types of antigenic stimulation induced IgG antibodies but with different precipitating and hemagglutinating capacities.
Footnotes
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↵1 This work was supported by grants AI-04342 and AI-05996 from the National Institutes of Health and from The National Foundation.
- Received February 11, 1966.
- Copyright © 1966 by The American Association of Immunologists, Inc.
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