There is growing appreciation that cellular metabolic and bioenergetic pathways do not play merely passive roles in activated leukocytes. Rather, metabolism has important roles in controlling cellular activation, differentiation, survival, and effector function. Much of this work has been performed in T cells; however, there is still very little information regarding mast cell metabolic reprogramming and its effect on cellular function. Mast cells perform important barrier functions and help control type 2 immune responses. In this study we show that murine bone marrow–derived mast cells rapidly alter their metabolism in response to stimulation through the FcεRI. We also demonstrate that specific metabolic pathways appear to be differentially required for the control of mast cell function. Manipulation of metabolic pathways may represent a novel point for the manipulation of mast cell activation.
B.P., L.A., G.M.D., and L.P.K. designed and analyzed experiments; B.P., L.A., and A.V.M. performed experiments; B.P. and L.P.K. wrote the manuscript; and G.M.D. edited the manuscript.
This work was supported by Public Health Service Grant R56AI067544 (to L.P.K.) and Sidney Kimmel Foundation for Cancer Research Grant SKF-015-039 (to G.M.D.).
The online version of this article contains supplemental material.
Abbreviations used in this article:
- bone marrow–derived mast cell
- extracellular acidification rate
- human serum albumin
- oxygen consumption rate
- spare respiratory capacity
- Received July 1, 2016.
- Accepted November 26, 2016.
- Copyright © 2017 by The American Association of Immunologists, Inc.