Naïve T cells recirculate between blood, lymph ducts and secondary lymphoid organs. Consequently, it should be different time that required for Ag-specific T cells, which are located in the various tissues, to populate and enter the draining lymph nodes in late time of the immune response. Late coming T cells were also primed in different environments and differentiated into memory T cells. We have investigated the kinetics of both OVA specific TCR Tg (OT-1 and OT-2) T cells stimulated earlier (early-stimulated T cells) and later in an immune response (late-stimulated T cells). To ascertain above studies, naïve T cells isolated from lymph nodes of OT-1/CD45.2+ mice and OT-2/CD45.2+ mice were injected i.v. into CD45.1+/CD45.2+ B6 host mice. For immunization of mice, ex-vivo expanded DCs that were induced from murine bone marrow cells by culturing with GM-CSF were incubated with OVA protein and LPS, and were injected s.c. into host mice. The early-stimulated T cells were developed by adoptive-transfer of Tg T cells into host mice before Ag-pulsed activated DCs were injected and the late-stimulated T cells were developed by adoptive-transfer of Tg T cells into mice 5-7 days after injection of Ag-pulsed activated DCs. The late-stimulated T cells divide slower and proliferate less than the early-stimulated T cells. These results suggest that the timing of recruitment of T cells into the draining lymph nodes affects T cells-fate that are influenced by different environments.
- Copyright © 2013 by The American Association of Immunologists, Inc.