Dengue virus (DENV) infection may result in severe dengue hemorrhagic fever (DHF). However, the mechanisms that cause hemorrhage in DHF are unclear. In this study, we demonstrated that antibodies against human thrombin were increased in the sera of dengue patients but not in other virus-infected patients. To further characterize the properties of these antibodies, affinity-purified anti-thrombin antibodies (ATA) were collected from DENV patients’ sera by thrombin and protein A/L affinity columns. We found human ATA bound not only to DENV, human thrombin, but also cross-reacted to human plasminogen (Plg). In addition, human ATA could inhibit not only thrombin activity but also enhance Plg activation in vitro. Taken together, these results suggest that antibodies induced by DENV, which contain both anti-thrombotic and pro-fibrinolytic activities, may contribute to the development of hemorrhage in DHF.
- Copyright © 2013 by The American Association of Immunologists, Inc.