To find new human gene encoding protein which has potential to be chemokine like or chemokine, our laboratory has established the peripheral blood mononuclear cell (PBMC) chemoattractant platform, which includes four steps. First, we screened out 212 genes potential to be cytokines from the genes library in which the genes’ cytokine-related function was unknown or unreported. And then, we constructed the eukaryotic vectors expressing the candidate genes. And third, the proteins encoded by the candidate genes, which could be secreted, were chosen. At last, the secretive proteins were applied on the PBMC chemoattractant platform to find the protein which had the chemoattractant ability. The PBMC chemoattractant platform was based on the Boyden Chamber Assay. Among the proteins which were potential to be chemokine or chemokine like, one protein was found having the ability to chemoattract peripheral blood monocyte and lymphocyte, which was low expressed in normal tissue and detected in human breast cancer and prostate cancer. What’s more, we have found that CCR2B was the protein’s chemoattractant receptor in vitro. In consideration of CCR2B is normally expressed in lots of cancer sites, the protein which we have found may play some kind role in the procession of cancer development.
- Copyright © 2013 by The American Association of Immunologists, Inc.