Immune response against Herpes simplex virus 1 (HSV-1) has been studied by various routes of inoculation in models of herpes. Zoteriform spread of HSV-1 infection occurs after primary inoculation of the skin of mice. This inoculation route has been used for study the pathogenesis of infection at nervous system level but little is known about the mechanism of immune response in this model. The aim of this work is to study the infiltrated of leukocytes in the inguinal lymph nodes (ILN) and correlate changes in leukocyte subpopulations with mortality in BALB/c mice cutaneously infected with HSV-1. We observed that infected mice showed 70-100% mortality during the acute infection. We removed inguinal and mesenteric lymph nodes (MLN) from infected mice and analysed phenotype of CD3, CD4, CD8, and CD19 cells from 0 to 15 days post-infection. The percentage of CD19+ cells and CD3+ cells in the ILN from infected mice changed from 20% to 50% and from 70% to 40% respectively. In contrast, such changes were not observed in MLN, suggesting that during acute infection immune response is elicited only at the region of the infection. Kinetic analysis of CD4+ and CD8+ T cells showed that the HSV-1-infection induced the expression of CD11c in T cells. Our findings show that HSV-1 infected mice retain a CD11c+ leukocyte infiltrate that could be involved in mortality. The role of CD11c+ cells will be further studied using immune-modulators that modify the percentage of those populations.
- Copyright © 2013 by The American Association of Immunologists, Inc.