Numerous inflammatory and fibrotic disorders arise from process malfunctions in fibroblast regulation of extracellular matrix remodeling and cytokine expression in which the Friend leukemia integration factor 1, an E26 transformation-specific family transcription factor, participates. We have previously shown that Fli-1 regulates inflammatory monocyte chemoattractant protein 1 expression in endothelial cells and Fli-1 is highly expressed in fibroblasts. In this report, we investigated the involvement of Fli-1 in extracellular matrix remodeling and regulation of inflammatory mediators in dermal fibroblasts. We isolated primary dermal fibroblasts from wild type and Fli-1 heterozygous C57/BL6 mice. Expression of Fli-1 in fibroblasts from Fli-1 heterozygous mice was reduced by half compared to expression in wild-type mice. We measured the concentration of MCP-1 in supernatants from dermal fibroblasts after simulation with lipopolysaccharide and found MCP-1 concentrations in Fli-1 heterozygous mice to be significantly increased compared with wild type controls. The concentrations of other inflammatory mediators including matrix metalloproteinase I in the supernatants are currently being studied. Our results suggest that Fli-1 is involved in regulating the expression of inflammatory mediators in dermal fibroblasts.
- Copyright © 2013 by The American Association of Immunologists, Inc.