Vaccines play an integral role in the fight against infectious diseases. Most current vaccines, however, were developed empirically, both in their formulation and schedule. A deeper understanding of the regulatory pathways involved in both adjuvanted vaccines and multi-injection schedules would contribute greatly to the rational design of future vaccines. Here we report a microarray analysis study of a 3 injection vaccination using Neisseria meningitidis PorB as a vaccine adjuvant and an Ova antigen in a murine model. We observe that the kinetics of the response in a number of gene sets is accelerated, with maximal expression after the second vaccination, rather than the third, in the adjuvanted vaccine. We also observe a recruitment of a number of innate inflammatory pathways, with adjuvanted vaccines eliciting more robust gene expression. Main effects and interactions between the antigen and adjuvant were determined, in particular as related and antibody production. In this report we demonstrate that the inclusion of PorB as an adjuvant into the formulation of a vaccine significantly alters the kinetics and nature of the immune response at a deep transcriptional level. This may provide meaningful implications for the rational design of future adjuvanted vaccines.
- Copyright © 2013 by The American Association of Immunologists, Inc.