The massive use of drugs on fish farming could have an important effect on immunological status of these organisms. Here we showed the effect of antibiotics used in salmon farming on the protein expression levels involved in metabolism and drug resistance in immunological cells from salmon head kidney (SHK-1 cells). The protein expression levels of CYP1A, CYP3A, FMO, GST, Pgp and MRP1 were measured by Western blot after antibiotic treatments. Cell viability was measured by MTT assays. Significant fluctuations of expression in the most proteins analyzed as response to antibiotic treatments were evidenced, while no effect on cell viability were observed. Therefore, the antibiotic treatment changes the expression levels of metabolizing and drug resistance proteins in these salmonid cells, which may be associated with the induction of drug resistance phenomena and a immunological response diminished. This information allows a deeper understanding of the mechanisms involved in the immunological status from a fish specie belonging to one of the most important economic sectors in Chile.
- Copyright © 2013 by The American Association of Immunologists, Inc.