Objective: We have shown that repeated immunization of mice with any antigen reproducibly led to development of SLE, in which a novel T cell type which we term an autoantibody-inducing CD4 T (aiCD4 T) cell was generated via TCR revision at periphery. The aiCD4 T cell induces varieties of autoantibodies and also help full maturation of CTL to induce lupus tissue injuries. We are trying tried to assign CD number on this aiCD4 T cell. Methods: BALB/c mice were repeatedly immunized with 12x OVA, KLH or SEB, and the expression of effector / memory markers on CD4 T cell was studied. CD4 T cells were isolated referring to CD45RB, CD27 and CD122 markers, and these cells were adoptively transferred into naïve mice. Autoantibodies in sera of recipient mice were measured 2 weeks after transfer. Microarray analyses were also performed using CD45RBlo122lo CD4 T cell immunized 12x with OVA. Results: The CD45RBlo, CD27lo and CD122hi CD4 T cells were significantly expanded in mice immunized 12x with either OVA, KLH or SEB. Adoptive transfer of CD45RBlo122lo CD4 T cells of mice immunized 12x with OVA showed that autoantibodies were significantly increased in naïve recipients. In microarray analysis, chemokine (C-C motif) receptor 4 (Ccr4) was increased >x4 in CD45RBlo122lo CD4 subset after 12x immunization with OVA. Conclusion: The aiCD4 T cell that induces SLE belongs to CCR4+CD45RBlo122lo CD4 subpopulation.
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