Salmonella enterica serovar Typhimurium are facultative intracellular bacteria that cause disease in numerous species, and infections resulting from poultry products are a major source of foodborne disease in humans. Young chicks are susceptible to Salmonella and heterophils, the avian equivalent to neutrophils, are involved in control of the bacteria. To identify simultaneous host-bacterial transcriptional changes, heterophils were isolated from 6-d-old chicks, exposed to S. typhimurium for 0-1h, total eukaryotic and prokaryotic RNA isolated from the same sample, and subjected to chicken whole genome and Salmonella microarrays. Heterophils exposed to S. typhimurium upregulated 2900 and downregulated 700 genes. Changes in S. typhimurium showed 1020-25 genes were upregulated and 846-967 downregulated. Gene ontology (GO) was evaluated on differentially expressed genes. Heterophils upregulated genes associated with regulation of cell differentiation, protein transport, macromolecule localization, and heterocycle metabolic processes and downregulated actin cytoskeleton organization and biogenesis, and cellular component organization and biogenesis. GO terms for upregulated S. typhimurium genes included fatty acid biosynthesis, flagellar assembly, glutathione metabolism, and the Type III secretion system. Beginning to characterize the host-pathogen interaction and global gene expression will provide insight into potential control mechanisms for this important foodborne pathogen.
- Copyright © 2013 by The American Association of Immunologists, Inc.