[Aim] PMDC05, a leukemic plasmacytoid dendritic cell (pDC) line which was established from CD4+CD56+ leukemia cells in our laboratory, showed a capacity of generating antigen-specific cytotoxic T lymphocytes (CTLs). In order to enhance an antigen presenting ability of PMDC05, PMDC05 was transduced with CD80 gene by lentiviral vector, which was named as PMDC11. We investigated whether PMDC11 could enhance allogeneic T cell response. [Materials and Methods] Surface antigen presentation-associated molecules and antigen presenting ability were analyzed in PMDC05 and PMDC11 with or without stimulation by phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (CI) by flow cytometry or mixed leukocyte culture (MLC). [Results] CD80 gene transduction induced a definite up-regulation of CD1a and HLA-DR in addition to CD80. In original PMDC05 cells, which had antigen presenting ability without any stimulation, PMA/CI could enhance the ability significantly. PMDC11 cells without stimulation had an antigen presenting ability equivalent to PMA/CI-stimulated PMDC05. Measurement of IFN-γ in supernatant of MLC demonstrated that PMDC11 stimulated allogeneic lymphocyte to produce much more IFN-γ compared with PMDC05. [Conclusion] Our data suggested PMDC11 cells, which were established by transducing CD80 gene into PMDC05 cells, could be efficiently applied as antigen presenting cells in adoptive cellular immunotherapy for tumors and severe infections.
- Copyright © 2013 by The American Association of Immunologists, Inc.