Allergic asthma is caused by inhaled allergens and is characterized by airway eosinophilia, as well as mucus hypersecretion, which can lead to airflow obstruction. Despite the association of increased IL-6 levels with human atopic asthma, the contribution of IL-6 to the development of allergic airway inflammation triggered by inhaled allergens remains unclear. In this study, we examined the role of IL-6 in a mouse model of allergic airway inflammation induced by direct airway exposure to extracts of Aspergillus fumigatus, a common allergen in humans. We show that inhaled A. fumigatus extracts rapidly trigger the production of IL-6 in the airways. IL-6 appears to be dispensable for the recruitment of eosinophils to the lung during the development of allergic airway inflammation. However, IL-6 is essential for mucus hypersecretion by airway epithelial cells triggered in response to inhaled A. fumigatus Ags. Impaired mucus production caused by IL-6 deficiency correlates with a severe reduction in the levels of IL-13, a major inducer of mucin glycoproteins. Thus, IL-6 is a key regulator of specific hallmark features of allergic airway inflammation and it could be a potential target for pulmonary diseases that are associated with goblet cell metaplasia and mucus hypersecretion.
Interleukin-6 is a multifunctional cytokine that is produced by a variety of hematopoietic and nonhematopoietic cell types in response to diverse stimuli (1). Although IL-6 was originally considered a surrogate marker of inflammation, similar to IL-1β and TNF-α, recent studies have shown that IL-6 influences the effector functions of various CD4+ T cell subsets. IL-6 inhibits Th1 differentiation through the up-regulation of SOCS1 (2) and promotes Th2 differentiation by the induction of NFAT (3) and c-Maf (4) during early CD4+ T cell activation. In addition, IL-6 induces the differentiation of Th17 effector cells in the presence of TGFβ (5, 6, 7). In contrast, it inhibits regulatory T cell development, most likely by suppressing Foxp3 expression (6, 8, 9). Its role in promoting Th2 and Th17 differentiation, along with inhibiting regulatory T cell activity, suggests that IL-6 might play a role in the onset and/or progression of diseases that are associated with these types of immune responses.
Allergic asthma is a chronic inflammatory disease of the airway that occurs in response to inhaled allergens such as ragweed pollen, cat dander, house dust mites, and fungi. The development of a CD4+ Th2 immune response and its associated cytokines (e.g., IL-4, IL-5, and IL-13) are known to play an important role in the pathogenesis of allergic asthma. IL-4 promotes Ig isotype switching in B cells to produce primarily IgE and IgG1 (10). IL-5 promotes eosinophil survival, differentiation, and migration (11), whereas IL-13 induces mucus metaplasia and airway hyperresponsiveness (12, 13, 14). Despite the role of Th17 cells in inflammation (15), the function of this subset and its signature cytokines IL-17A and IL-17F in allergic airway inflammation is less clear. IL-17R-deficient mice exhibit a decrease in eosinophil recruitment, Th2 cytokine production, and IgE levels during induction of allergic airway inflammation, suggesting that IL-17 is required during the initiation of allergic asthma (16, 17). However, exogenous administration of IL-17 during allergen challenge decreases local Th2 cytokine production and eosinophilia through repressing eotaxin production by lung epithelial cells (16).
Despite the role of IL-6 in Th2 and Th17 differentiation, its relative contribution to allergic airway inflammation remains unclear. Correlation studies in patients with asthma have shown increased IL-6 levels in bronchoalveolar lavage fluid (BALF)3 (18) and serum (19), as well as increased IL-6 production from cultured lung epithelial cells (20), suggesting a role for IL-6 in asthma pathogenesis. However, studies in mice have yielded conflicting results. Studies examining acute or chronic allergic airway inflammation induced by i.p. OVA immunization with alum followed by challenge with aerosolized OVA in IL-6 knockout (KO) mice show increased airway eosinophilia and Th2 cytokine production, suggesting that IL-6 may negatively regulate allergic airway inflammation (21, 22). In contrast, local disruption of IL-6 signaling in the lung by intranasal administration of an IL-6Rα-blocking Ab before OVA challenge decreases OVA-induced Th2 inflammation in vivo, suggesting that IL-6 positively regulates the Th2 immune response (23). Overall, whether IL-6 promotes or inhibits Th2 inflammation or even acts in a dual fashion in allergen-induced airway inflammation remains unclear.
In this study, we examined the role of IL-6 in allergic airway inflammation induced by repeated direct exposure of the lungs to extracts of Aspergillus fumigatus (A.f.), a common spore-forming fungi and known allergen in humans (24), without previous i.p. immunization. We show that IL-6 is essential specifically for the development of mucus metaplasia in the airways in response to inhaled allergen, but it is dispensable for the recruitment of eosinophils to the lung. Thus, the elevation of IL-6 found in some respiratory diseases (e.g., chronic obstructive pulmonary disease and allergic asthma) could contribute to the pathogenesis of the disease by promoting mucus hypersecretion.
Materials and Methods
C57BL/6J mice were purchased from The Jackson Laboratory. IL-6-deficient mice (IL-6−/−) generated by deletion of exon I, II, and III have been previously described (25) and were obtained from Dr. G. Ciliberto (Istituto di Ricerche di Biologia Moleculare P. Angeletti, Rome, Italy). IL-21-deficient mice have been previously described (26). All mice were housed under sterile conditions at the animal care facility at the University of Vermont (Burlington, VT). All procedures performed on the mice were approved by the University of Vermont Institutional Animal Care and Use Committee. Mice were administered oropharyngeal (o.p.) 5 μg of A.f. extracts in PBS as previously described (27). For quantification of early cytokine/chemokine production, BALF was collected 6 or 24 h after A.f. dose. For analysis of allergic airway inflammation, mice were administered A.f. extracts on days 0, 7, and 14. Mice were euthanized 48 h after the last challenge and tissue was processed for analysis.
IL-6 levels in serum were measured after 6 h of i.p. immunizations of wild-type mice with 25 μg of OVA (Sigma-Aldrich) and either 2.25 mg of alum (Thermo Scientific) or 15 μg or A.f. extracts.
BALF collection, cell count, and cell differential
Cold PBS (1 ml) was instilled into the lungs as previously described (27). Cells were centrifuged and counted on the Advia cell counter (Bayer). Cells (5 × 104) were cytospun and stained with hema-3 (Biochemical Sciences). Two hundred cells per high power field were counted and classified as macrophages, neutrophils, lymphocytes, or eosinophils by cell morphology and staining.
CD4+ T cell purification and activation
Splenic CD4+ T from A.f.-exposed animals were negatively selected as previously described (3). Briefly, cells expressing CD8, CD11b, NK1.1, and MHC class II were depleted using specific mAbs (BD Pharmingen) and Ig-coated magnetic beads (Qiagen). Isolated CD4+ T cells (1 × 106) were activated with 5 μg/ml plate-bound anti-CD3 (2C11) and 1 μg/ml soluble anti-CD28 (BD Pharmingen) mAbs for 24 h.
Lung CD4+ T cells were purified from lung cell homogenates by sorting on a FACS-Aria (BD Biosciences) for CD4 (CD4+ T cells) and CD1d-tetramer (CD1d tetramer negative to discard NKT cells) expression. Cells were restimulated with A.f. extracts (5 μg/ml) in the presence of APCs (T cell-depleted spleen cells) for 72 h.
Cytokine and Ig detection
Formalin-fixed, paraffin-embedded sections were prepared as previously described (28). Sections were stained for H&E and periodic acid-Schiff (PAS) according to routine procedures. Three investigators blinded to each experimental group scored airway inflammation and mucus production in the following way: 0, no inflammation or mucus production; 1, mild inflammation or mucus production; 2, moderate inflammation or mucus production with multiple airway involvement; and 3, severe inflammation or mucus production with majority of airways involved. Images were obtained using the Olympus BX50 light microscope with an Optronics Magnafire digital camera.
Total RNA was extracted from whole lung tissue and CD4+ T cells by using the RNAeasy kit (Qiagen) as recommended by the manufacturer. First-strand cDNA synthesis was performed as previously described (27). Quantitative PCR was performed on cDNA using Assay on Demand probe/primer sets for IL-5, IL-13, IL-17, IL-21, Muc5AC, 18S, and β2-microglobulin (Applied Biosystems). Gene amplification was performed on an ABIPrism 7700 instrument from Applied Biosystems. Expression of target genes in the lung and CD4+ T cells was normalized to 18S and β2-microglobulin levels, respectively. Relative values were determined by the comparative Ct method.
The data are presented as the mean ± SEM. The significance of differences between two groups was determined by the Mann-Whitney U test with GraphPad Prism (v. 5.0). For all analyses, p < 0.05 is considered statistically significant and anything greater than this value is not significant.
IL-6 is rapidly produced in the lung in response to A. fumigatus exposure
Allergen exposure in human subjects leads to increased IL-6 levels in BALF and sputum (18, 29). Several studies have shown a role of IL-6 in the differentiation of CD4+ T cells into specific effector cell subsets. Thus, IL-6 produced in the lung upon allergen exposure could modulate the type of CD4+ T cell response that develops against inhaled Ags. Fungi (e.g., A. f. and A. alternata) are commonly encountered environmental allergens and act as asthma triggers (30). We have previously shown that direct lung exposure to A.f. extracts by o.p. administration in the absence of adjuvant is sufficient to induce allergic airway inflammation characterized by eosinophilia, mucus hypersecretion, and Th2 cytokine production (27). To determine whether A.f. exposure induces IL-6 production in the lung, IL-6 levels were examined in BALF from unexposed mice and mice treated o.p. with A.f. extracts. As early as 6 h postexposure, high levels of IL-6 were present in BALF, suggesting that this cytokine could affect CD4+ T cell differentiation (Fig. 1⇓A). We also examined the presence of other cytokines known to affect the differentiation of CD4+ T cells into specific effector Th cells. Although TNF-α and specific chemokines (e.g., KC and MIP-1α) were also induced (Fig. 1⇓B), none of the established polarizing cytokines such as IL-4 (Th2 polarizing), IL-12, or IFN-γ (Th1 polarizing) were detected in the BALF of A.f.-exposed mice (data not shown). Thus, direct A.f. exposure to the lung selectively triggered the production of IL-6 among the polarizing cytokines.
Although IL-6 is considered as an inflammatory cytokine that is induced early during the immune response by a number of adjuvants (31, 32), recent studies have shown that alum, a commonly used adjuvant, activates the inflammasome pathway and induces IL-1β and IL-18, but has minimal effect on IL-6 (33, 34). To further show the specific effect of A.f. on IL-6 production during the initiation of the immune response, mice were i.p. immunized with OVA together with alum or A.f. extracts and IL-6 levels in serum were determined. In agreement with previous studies on alum, the level of IL-6 in the serum of mice administered the adjuvant was minimal at 6 h postinjection (Fig. 1⇑C) and undetectable after 12 h (data not shown). In contrast, i.p. immunization with A.f. extracts induced significant IL-6 production (Fig. 1⇑C). Thus, components within natural allergens favor IL-6 production.
Airway eosinophilia does not require IL-6
The above studies indicate that direct airway exposure to allergens such as A.f. triggered rapid production of IL-6 in mice, as previously described in human subjects. Considering the known role of IL-6 in Th1, Th2, and Th17 differentiation, we investigated the contribution of this cytokine to the different pathological features of allergic airway inflammation induced by inhalation of A.f. extracts. We used IL-6−/− mice that were previously generated by deletion of exons I, II, and III of the IL-6 gene (25). Wild-type and IL-6−/− mice were administered A.f. extracts o.p. on days 0, 7, and 14. Allergic airway inflammation was examined 48 h after the last dose. The total cell count in the BALF was similar in A.f.-exposed wild-type and in IL-6−/− mice (Fig. 2⇓A). Cell differential analysis in BALF (Fig. 2⇓B) and total eosinophil count (Fig. 2⇓C) showed that the accumulation of these cells were also comparable in wild-type and IL-6−/− mice. In addition, H&E-staining analysis showed similar peribronchial and perivascular eosinophilic inflammation (Fig. 2⇓, D and E). Thus, IL-6 deficiency does not interfere with the recruitment of eosinophils to the lung.
Because IL-5 is one of the major factors that promotes airway eosinophilia following allergen exposure (35), it was possible that IL-6 deficiency did not affect the levels of this cytokine. However, a reduction of IL-5 expression was detected in the IL-6−/− mice (Fig. 2⇑F), suggesting that some other factors could compensate for the impaired IL-5 production. Eotaxin is also a potent chemotactic factor for eosinophils (36). Surprisingly, eotaxin levels in the BALF were significantly higher in IL-6−/− mice compared with wild-type mice (Fig. 2⇑G). Thus, increased levels of eotaxin could compensate for the impaired IL-5 production and support eosinophil recruitment in IL-6−/− mice.
Eotaxin is primarily produced by epithelial cells (36) and there is no evidence suggesting that IL-6 has a direct negative effect on eotaxin production by these cells. However, recent studies have shown that the neutrophil chemoattractant IL-17 interferes with eosinophil recruitment by inhibiting the production of eotaxin by epithelial cells (16). IL-6, together with TGFβ, is a major promoting factor for the differentiation of Th17 cells and has been shown to be required for the development of Th17 cells in autoimmune disease (5, 6, 7). Thus, increased eotaxin in IL-6−/− mice could be due to impaired IL-17 production. We therefore examined whether A.f.-induced allergic airway inflammation leads to increased IL-17 production and whether loss of IL-6 reduces the Th17 response. IL-17 was clearly expressed in CD4+ T cells from A.f.-exposed wild-type mice, but it was almost undetectable in CD4+ T cells from IL-6−/− mice (Fig. 2⇑H). Thus, IL-6 deficiency also impairs development of Th17 cells in A.f.-induced allergic airway inflammation. Reduced IL-17 levels could be responsible for the up-regulation of eotaxin in these mice.
Increased IgE levels in A.f.-exposed IL-6−/− mice correlate with the requirement of IL-6 for IL-21 production
Because another hallmark feature of allergic airway inflammation is the production of the Ab isotypes IgE and IgG1 (37), we measured serum Ab levels from A.f.-exposed mice. IL-6−/− mice showed significantly lower circulating levels of IgG1 than wild-type mice in response to A.f. (Fig. 3⇓A). However, serum IgE levels in A.f.-exposed IL-6−/− mice were elevated compared with the levels in exposed wild-type mice (Fig. 3⇓B). IL-6 therefore contributes to IgG1 production during the induction of allergic airway inflammation, although it seems to have a negative effect on IgE production.
The Th2 cytokine IL-4 is a critical factor that drives Ab production in allergic airway inflammation because it promotes B cell Ig class switching to IgE, IgG1, and IgG2b (38, 39). Because IL-6 induces IL-4 production by CD4+ T cells (40, 41), we examined IL-4 levels in splenic CD4+ T cells isolated from A.f.-exposed wild-type and IL-6−/− mice upon ex vivo restimulation. Levels of IL-4 by CD4+ T cells from IL-6−/− mice were decreased compared with cells from wild-type mice (Fig. 3⇑C). The levels of IFN-γ were also examined in these cells. Although very low, levels were comparable between IL-6−/− and wild-type cells (Fig. 3⇑D). In addition, we purified lung-specific CD4+ T cells from A.f.-exposed wild-type and IL-6−/− mice and restimulated them ex vivo with Ag for 72 h. Lung CD4+ T cells from IL-6−/− mice also produce less IL-4 (Fig. 3⇑E), but more IFN-γ (Fig. 3⇑F), than CD4+ T cells from wild-type mice, further supporting the role of IL-6 in promoting Th2 while inhibiting Th1 CD4+ T cell differentiation.
Although the reduced production of IL-4 could explain the decreased IgG1 production, it could not account for the increased IgE levels observed in the IL-6−/− mice. Similar to IL-4, IL-21 induces plasma cell differentiation and regulates Ab production by promoting IgG1, IgG2b, and IgG3 isotype switching (42, 43). However, it negatively regulates IgE production (42, 43, 44) by inducing the Id2 gene expression in B cells (45). We have recently shown that IL-6 alone is a highly specific and effective inducer of IL-21 in naive and memory CD4+ T cells and it is required for IL-21 production in vivo (26). To test whether enhanced IgE levels in A.f.-exposed IL-6−/− mice were due to impaired IL-21 production, IL-21 levels in CD4+ T cells purified from A.f.-exposed wild-type and IL-6−/− mice were measured. IL-21 was expressed in CD4+ T cells from A.f.-exposed wild-type mice (Fig. 4⇓A), but it was almost undetectable in CD4+ T cells from IL-6−/− mice (Fig. 4⇓A). Thus, the effect of IL-6 deficiency on IgE production during A.f.-induced allergic airway inflammation is likely an indirect effect caused by insufficient IL-21 production from CD4+ T cells.
IL-21R-deficient mice have been shown to have increased baseline IgE levels due to the lack of IL-21 signaling (42). Similarly, analysis of serum IgE levels in unexposed IL-21−/− mice showed increased baseline IgE levels (Fig. 4⇑B). To determine whether IL-6 deficiency could also lead to increased IgE secretion, we examined the basal levels of IgE in IL-6−/− mice. Similar to IL-21−/− mice, the IgE levels were significantly elevated in naive IL-6−/− mice (Fig. 4⇑B). Together, these data indicate that both IL-21 and IL-6 deficiency result in systemic IgE hypersecretion and thereby increased IgE levels during allergic airway inflammation.
IL-6 is essential for mucus production by lung epithelial cells
A major feature of allergic airways disease is mucus metaplasia and mucus hypersecretion by airway epithelium. Excessive mucus production can lead to physical obstruction of smaller caliber airways (46). Goblet cell hyperplasia and increased mucus production require the adaptive immune response. Although mucus hypersecretion and eosinophilia in the lung are normally associated, they are two independent pathological events. We therefore examined the role of IL-6 in mucus production in the airway during allergic airway inflammation. Lung sections from A.f.-exposed wild-type and IL-6 −/− mice were stained for PAS to visualize mucus production by airway epithelial cells. The PAS score was based on the intensity of PAS staining per airway and the total number of positive airways. Both the number of airways with mucus-producing cells and the number of mucus-producing cells per airway were strongly reduced in IL-6−/− mice compared with wild-type mice (Fig. 5⇓, A and B), indicating that IL-6−/− mice were more resistant to developing mucus metaplasia. The impaired mucus production was further confirmed by examining the expression of the mucus glycoprotein Muc5AC in the lung by real-time RT-PCR. High levels of Muc5AC were present in A.f.-exposed wild-type mice whereas only marginal Muc5AC levels were detected in IL-6−/− mice (Fig. 5⇓C). Thus, IL-6 is essential for mucus production in the airway.
Because IL-13 is the major cytokine involved in mucus production by lung epithelial cells (12, 13, 47), we investigated the effect of IL-6 deficiency on IL-13 expression during allergic airway inflammation. Correlating with the inability to produce mucus in the airway, a profound reduction in IL-13 expression in the lung was observed in IL-6−/− mice relative to wild-type mice (Fig. 5⇑D). IL-13 is a Th2 type of cytokine that is primarily induced by IL-4 (48). To determine whether IL-6 could also directly promote IL-13 production by CD4+ T cells, naive CD4+ T cells were activated in vitro with anti-CD3 and anti-CD28 mAbs in the presence or absence of IL-6 or IL-4. IL-13 in the culture supernatant was measured after 48 h. CD4+ T cells activated in the absence of exogenous cytokine produced marginal levels of IL-13. IL-4 had a minimal effect on IL-13 levels at this time of activation (Fig. 5⇑E). In contrast, IL-6 strongly up-regulated IL-13 production (Fig. 5⇑E). Thus, IL-6 directly induces IL-13 production by CD4+ T cells during activation, providing a potential mechanism by which IL-6 enhances mucus production in the airways following allergen exposure.
Although IL-6 has been shown to be elevated in allergic asthma in humans, its exact contribution to the hallmark features of this disease remains unclear. Correlative studies have provided evidence that IL-6 is up-regulated in the airway and systemic circulation of asthmatic patients (18, 19, 49, 50, 51, 52, 53). However, the traditional view of IL-6 as a proinflammatory biomarker together with TNF-α and IL-1β suggests that increased IL-6 levels in asthma could merely be a result of an ongoing inflammatory response, rather than serving a functional role. Recently, we have shown that there is a selective increase in IL-6, but not TNF-α and IL-1β, in the large central airways of mildly allergic asthmatic patients compared with healthy control subjects (W. A. Neveu, J. Allard, D. Raymond, L. Bourassa, S. Burns, J. Bunn, C. Irvin, D. Kaminsky, M. Rincon, submitted for publication). This increase in IL-6 inversely associates with airflow obstruction. Similar observations were made in a patient cohort that included asthmatics with more severe disease (54). Together, these data suggest that IL-6 influences pathological changes within the airway that results in increased airflow resistance. In this study, using null IL-6−/− mice, we show for the first time that indeed IL-6 is essential for mucus production during allergic airway inflammation.
Increased mucus production from lung epithelium in chronic inflammatory diseases such as asthma can physically obstruct the airway, resulting in an increase in airflow resistance (46, 55). The lung uses a homeostatic mechanism that balances mucus production with airway clearance (56). However, if either of these components are perturbed, it can lead to lung dysfunction. Thus, the presence of IL-6 in the asthmatic airway could contribute to lung function through induction of mucus hypersecretion. The effect of IL-6 on mucus production may be a consequence of IL-6 regulating IL-13 expression in the lung and IL-13 inducing mucus cell metaplasia in the airway. Alternatively, because in vitro studies have shown that IL-6 can directly induce the mucin genes Muc5AC and Muc5B in human lung epithelial cell lines, we cannot exclude the possibility that IL-6 may have a direct effect on mucus production by lung epithelial cells during allergic airway inflammation (57).
The role of IL-6 in the development of allergen-induced airway eosinophilia remains unclear. In vivo blockade of IL-6 signaling by the administration of an anti-IL-6R Ab during the challenge phase with the OVA Ag seems to decrease the accumulation of eosinophils in the lung (23). However, previous studies using the OVA model in IL-6 KO mice showed increased eosinophil accumulation in the lungs of these mice (21, 22). In our study, using the A.f. exposure system, we show no significant difference in eosinophilia between IL-6−/− mice and wild-type mice. This apparent discrepancy could be due to the different experimental models (OVA vs A.f.). Alternatively, it could also be due to the use of IL-6-targeted mice that were generated by different genetic approaches. In our study, we used IL-6−/− mice that were previously generated by deletion of Exons I, II, and III of the IL-6 gene (25). The IL-6 KO mice used in the previous studies were generated by insertion of the neomycin-resistance cassette in Exon 2 in the same orientation as the IL-6 gene (58), leading to increased levels of IL-6 mRNA that may encode for a potential rIL-6. We also examined the response to A.f. exposure in those IL-6 KO mice and found a significantly higher number of eosinophils in the airway in these mice than in wild-type mice (data not shown), corroborating the observations that were previously published using the OVA model (21, 22). Thus, the apparent difference in the two studies is likely due to the difference in the genetically manipulated mouse models used to disrupt IL-6 gene expression.
In this paper, we describe for the first time that IL-6 deficiency leads to hyper-IgE production, and it is more pronounced upon induction of allergic airway inflammation. This phenotype resembles that of mice deficient for IL-21. Considering the suppressive effect of IL-21 on IgE production, partly mediated by Id2 activity (45), and the selective induction of IL-21 expression by IL-6 (59), we propose that increased IgE production in IL-6−/− mice is caused by insufficient IL-21 production by CD4+ T cells. The strong reduction of IL-21 levels in IL-6−/− mice during allergic airway inflammation support this model. Although the production of IL-4 is also impaired in these mice, remaining IL-4 in the absence of the suppressive effect of IL-21 could be sufficient to trigger enhanced IgE production. Alternatively, it is possible that both IL-6 and IL-21 use similar signaling pathways to repress IgE production independent of one another. Interestingly, the primary immunodeficiency hyper-IgE syndrome has been genetically linked to mutations that lead to the generation of a dominant negative mutant of the transcription factor STAT3 (60, 61). Because STAT3 is activated by either IL-6 or IL-21, deficiency of one these cytokines may be sufficient to prevent STAT3 activation and thereby lead to hyper-IgE production. Interestingly, both IL-21 and IL-6 deficiencies lead to an increase in basal IgE levels in the serum that is similar to the phenotype of hyper-IgE syndrome.
In summary, the results from this study show that IL-6 is rapidly produced in the lung upon allergen exposure and that it plays a key role in dictating the immune response against allergens, as well as in specific pathological features of allergic airway inflammation, primarily mucus production.
We thank Kieren Marr (Fred Hutchinson Cancer Center, University of Washington, Seattle, WA) for providing us with the A.f. extracts. We thank Timothy Hunter and Mary Lou Shane (DNA Sequencing Facility, University of Vermont, Burlington, VT) for assistance with real-time RT-PCR analysis and Colette Charland for cell sorting. We also thank Charlie Irvin, Matthew Poynter, Elizabeth Bonney, Janice Bunn, and Juan Anguita for helpful discussion.
The authors have no financial conflict of interest.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵1 This research was supported by the National Institutes of Health Grant R01HL69136 (M.R.) and the Center for Biomedical Research Excellence Program of the National Center for Research Resources Grant P20RR15557 (L.W.).
↵2 Address correspondence and reprint requests to: Dr. Mercedes Rincon, Department of Medicine, Division of Immunobiology, Given Medical Building D305, University of Vermont, 89 Beaumont Avenue, Burlington, VT 05405. E-mail address:
↵3 Abbreviations used in this paper: BALF, bronchoalveolar lavage fluid; A.f., Aspergillus fumigatus; KO, knockout; o.p., oropharyngeal; PAS, periodic acid-Schiff.
- Received September 3, 2008.
- Accepted May 25, 2009.
- Copyright © 2009 by The American Association of Immunologists, Inc.