Skip to main content

Main menu

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Top Downloads
    • Annual Meeting Abstracts
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • Rights and Permissions
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons

User menu

  • Subscribe
  • Log in

Search

  • Advanced search
The Journal of Immunology
  • Other Publications
    • American Association of Immunologists
    • ImmunoHorizons
  • Subscribe
  • Log in
The Journal of Immunology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Next in The JI
    • Archive
    • Brief Reviews
    • Pillars of Immunology
    • Translating Immunology
    • Top Downloads
    • Annual Meeting Abstracts
  • Info
    • About the Journal
    • For Authors
    • Journal Policies
    • Influence Statement
    • Rights and Permissions
    • For Advertisers
  • Editors
  • Submit
    • Submit a Manuscript
    • Instructions for Authors
    • Journal Policies
  • Subscribe
    • Journal Subscriptions
    • Email Alerts
    • RSS Feeds
    • ImmunoCasts
  • More
    • Most Read
    • Most Cited
    • ImmunoCasts
    • AAI Disclaimer
    • Feedback
    • Help
  • Follow The Journal of Immunology on Twitter
  • Follow The Journal of Immunology on RSS

STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-κB p65

Toshiyuki Kawashima, Ken Murata, Shizuo Akira, Yukio Tonozuka, Yukinori Minoshima, Sizhou Feng, Hidetoshi Kumagai, Hiromichi Tsuruga, Yasuo Ikeda, Shigetaka Asano, Tetsuya Nosaka and Toshio Kitamura
J Immunol October 1, 2001, 167 (7) 3652-3660; DOI: https://doi.org/10.4049/jimmunol.167.7.3652
Toshiyuki Kawashima
Division of Hematopoietic Factors and Division of Hematology, Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ken Murata
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shizuo Akira
Department of Host Defense, Core Research for Engineering, Science, and Technology of Japan Science and Technology Corporation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yukio Tonozuka
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yukinori Minoshima
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sizhou Feng
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hidetoshi Kumagai
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiromichi Tsuruga
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuo Ikeda
Division of Hematology, Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shigetaka Asano
Department of Hematology/Oncology, Institute of Medical Science, University of Tokyo, Tokyo, Japan;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsuya Nosaka
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshio Kitamura
Division of Hematopoietic Factors and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

We recently demonstrated that STAT5 can induce a variety of biological functions in mouse IL-3-dependent Ba/F3 cells; STAT5-induced expression of pim-1, p21WAF/Cip1, and suppressor of cytokine signaling-1/STAT-induced STAT inhibitor-1/Janus kinase binding protein is responsible for induction of proliferation, differentiation, and apoptosis, respectively. In the present study, using a constitutively active STAT5A (STAT5A1*6), we show that STAT5 induces macrophage differentiation of mouse leukemic M1 cells through a distinct mechanism, autocrine production of IL-6. The supernatant of STAT5A1*6-transduced cells contained sufficient concentrations of IL-6 to induce macrophage differentiation of parental M1 cells, and STAT3 was phosphorylated on their tyrosine residues in these cells. Treatment of the cells with anti-IL-6 blocking Abs profoundly inhibited the differentiation. We also found that the STAT5A1*6 transactivated the IL-6 promoter, which was mediated by the enhanced binding of NF-κB p65 (RelA) to the promoter region of IL-6. These findings indicate that STAT5A cooperates with Rel/NF-κB to induce production of IL-6, thereby inducing macrophage differentiation of M1 cells in an autocrine manner. In summary, we have shown a novel mechanism by which STAT5 induces its pleiotropic functions. Cytokines

  • Received November 14, 2000.
  • Accepted July 19, 2001.
  • Copyright © 2001 by The American Association of Immunologists
View Full Text
PreviousNext
Back to top

In this issue

The Journal of Immunology: 167 (7)
The Journal of Immunology
Vol. 167, Issue 7
1 Oct 2001
  • Table of Contents
  • About the Cover
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Immunology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-κB p65
(Your Name) has forwarded a page to you from The Journal of Immunology
(Your Name) thought you would like to see this page from the The Journal of Immunology web site.
Citation Tools
STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-κB p65
Toshiyuki Kawashima, Ken Murata, Shizuo Akira, Yukio Tonozuka, Yukinori Minoshima, Sizhou Feng, Hidetoshi Kumagai, Hiromichi Tsuruga, Yasuo Ikeda, Shigetaka Asano, Tetsuya Nosaka, Toshio Kitamura
The Journal of Immunology October 1, 2001, 167 (7) 3652-3660; DOI: 10.4049/jimmunol.167.7.3652

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-κB p65
Toshiyuki Kawashima, Ken Murata, Shizuo Akira, Yukio Tonozuka, Yukinori Minoshima, Sizhou Feng, Hidetoshi Kumagai, Hiromichi Tsuruga, Yasuo Ikeda, Shigetaka Asano, Tetsuya Nosaka, Toshio Kitamura
The Journal of Immunology October 1, 2001, 167 (7) 3652-3660; DOI: 10.4049/jimmunol.167.7.3652
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Innate Immunity Together with Duration of Antigen Persistence Regulate Effector T Cell Induction
  • Regulatory Roles of IL-2 and IL-4 in H4/Inducible Costimulator Expression on Activated CD4+ T Cells During Th Cell Development
  • Induction of CD4+ T Cell Apoptosis as a Consequence of Impaired Cytoskeletal Rearrangement in UVB-Irradiated Dendritic Cells
Show more CELLULAR IMMUNOLOGY AND IMMUNE REGULATION

Similar Articles

Navigate

  • Home
  • Current Issue
  • Next in The JI
  • Archive
  • Brief Reviews
  • Pillars of Immunology
  • Translating Immunology

For Authors

  • Submit a Manuscript
  • Instructions for Authors
  • About the Journal
  • Journal Policies
  • Editors

General Information

  • Advertisers
  • Subscribers
  • Rights and Permissions
  • Public Access
  • Privacy Policy
  • Disclaimer

Journal Services

  • Email Alerts
  • RSS Feeds
  • ImmunoCasts
  • Twitter

Copyright © 2019 by The American Association of Immunologists, Inc.

Print ISSN 0022-1767        Online ISSN 1550-6606