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Serum Opsonic Activity in Rodent Malaria: Functional and Immunochemical Characteristics in Vitro

Kenneth W. Hunter Jr., Jerry A. Winkelstein and Thomas W. Simpson
J Immunol December 1, 1979, 123 (6) 2582-2587;
Kenneth W. Hunter
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Jerry A. Winkelstein
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Thomas W. Simpson
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Abstract

The functional and immunochemical characteristics of serum opsonic activity in rodent malaria were examined in the present study. Schizont- and late trophozoite-enriched populations of Plasmodium berghei-infected red blood cells (IRBC) were isolated on a Ficoll density-gradient and used in an in vitro phagocytosis system composed of serum and monolayer cultures of rat peritoneal macrophages. Hyperimmune serum augmented the phagocytosis of IRBC to a greater degree than did nonimmune serum. When either IRBC or macrophages were pre-incubated with serum, the phagocytosis-promoting factors acted on the IRBC rather than on the macrophages in a manner characteristic of serum opsonins. The opsonic activity was specific for IRBC since noninfected red blood cells were rarely phagocytized and were unable to absorb opsonic activity from serum. The opsonic activity of both hyperimmune and nonimmune sera was heat stable, and unaffected by agents known to inactivate or inhibit complement (cobra venom factor and ethylenediaminetetraacetic acid). Finally, the opsonic activity was identified in preparations of purified IgG isolated from both hyperimmune and nonimmune sera.

Footnotes

  • ↵2 Jerry A. Winkelstein is an investigator of the Howard Hughes Medical Institute.

  • ↵1 This work was supported in part by United States Public Health Service Grants AI-11637 and 5T01-A1-00020, and a grant from the John W. Graham Fund, the Johns Hopkins University.

  • Received July 11, 1979.
  • Accepted August 22, 1979.
  • Copyright © 1979 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 123, Issue 6
1 Dec 1979
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Serum Opsonic Activity in Rodent Malaria: Functional and Immunochemical Characteristics in Vitro
Kenneth W. Hunter, Jerry A. Winkelstein, Thomas W. Simpson
The Journal of Immunology December 1, 1979, 123 (6) 2582-2587;

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Serum Opsonic Activity in Rodent Malaria: Functional and Immunochemical Characteristics in Vitro
Kenneth W. Hunter, Jerry A. Winkelstein, Thomas W. Simpson
The Journal of Immunology December 1, 1979, 123 (6) 2582-2587;
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Print ISSN 0022-1767        Online ISSN 1550-6606