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Specific Partial Depletion of Graft-vs-Host Activity by Incubation and Centrifugation of Mouse Spleen Cells on Allogeneic Spleen Cell Monolayers

Michael G. Mage and Louise L. McHugh
J Immunol October 1, 1975, 115 (4) 911-913;
Michael G. Mage
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Louise L. McHugh
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Abstract

Spleen cells (from BALB/c mice immunized with the C57BL/6 lymphoma EL4, or from non-immune BALB/c) were incubated on monolayers of [C57BL/6 × BALB/cF1 (B6CF1) spleen cells on polylysine-coated polystyrene Petri plate, for ½ hr or for 1 hr at 37°C followed by centrifugation of the monolayers for 5 min at 70 × G to 110 × G at 34 to 37°C. Control monolayers were BALB/c spleen cells. As measured by the Simonsen spleen weight assay in neonatal mice, graft-vs-host (GVH) activity was partially depleted in cell populations nonadherent to B6CF1 monolayers. Residual GVH activity of these nonadherent cells was about half that of cells incubated on the control syngeneic monolayers (the mean of eight experiments was 49% ± 11% S.D.). Two or three consecutive cycles of incubation and centrifugation did not significantly diminish the residual GVH activity, suggesting that spleen cells with GVH activity are heterogeneous with respect to binding to allogeneic target cells under the above conditions. Cell populations nonadherent to third-part [A × AL]F1 monolayers retained full activity, and cell populations partially depleted of GVH activity in B6CF1 neonates had full activity in third-party [BALB/c × AL]F1 neonates.

  • Received May 5, 1975.
  • Copyright © 1975 by The American Association of Immunologists, Inc.

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The Journal of Immunology
Vol. 115, Issue 4
1 Oct 1975
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Specific Partial Depletion of Graft-vs-Host Activity by Incubation and Centrifugation of Mouse Spleen Cells on Allogeneic Spleen Cell Monolayers
Michael G. Mage, Louise L. McHugh
The Journal of Immunology October 1, 1975, 115 (4) 911-913;

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Specific Partial Depletion of Graft-vs-Host Activity by Incubation and Centrifugation of Mouse Spleen Cells on Allogeneic Spleen Cell Monolayers
Michael G. Mage, Louise L. McHugh
The Journal of Immunology October 1, 1975, 115 (4) 911-913;
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Print ISSN 0022-1767        Online ISSN 1550-6606