Abstract
An in vitro assay of delayed hypersensitivity in man, modified from the method of Søborg and Bendixen, was evaluated. This assay is based on the inhibition of migration of human peripheral leukocytes in the presence of specific soluble antigen. Twelve of 13 individuals who were skin test positive to tuberculin showed inhibition of leukocyte migration when incubated with 100 µg/ml of soluble purified protein derivative. Only 1 of 12 skin test negative individuals showed a similar inhibition of leukocyte migration. Analysis of the migrating populations showed that the migration of mononuclear cells and not of neutrophils was inhibited.
Footnotes
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↵1 This work was supported in part by a Research Training Grant from the National Institutes of Health and grants from the United States Army and the Atomic Energy Commission, and by AI-07685, Cancer Contract Grant NIH 69-2061, and AM-5577-09 from the N. I. H.
- Received July 2, 1970.
- Copyright, 1970, by The Williams & Wilkins Company
- Copyright © 1970 by The American Association of Immunologists, Inc.
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