HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Flisser, A. Articles by Garcia, F. PubMed Articles by Flisser, A. Articles by Garcia, F.

Intestinal immunity in the experimental model of Taenia solium taeniosis

¹ Facultad de Medicina, UNAM, Mexico DF, 04510, Mexico, ² Instituto Nacional de Pediatría, SSA, Mexico DF, 04530, Mexico

Abstract

Taenia solium can grow in normal hamsters, mature worms are obtained. Expulsion starts at 3 weeks and parasites may remain up to 2 months. An inflammatory reaction in the intestinal mucosa, surrounding the scolex of the worm, is produced; it comprised lymphocytes, plasma cells, eosinophils and goblet cells. Presently we are searching for mRNA of Th1 and Th2 interleukins by in situ hybridization in intestinal biopsies. For this purpose hamsters were infected per os with 8 T. solium cysticerci, necropsies were performed at different days post-infection. One cm biopsies of the small intestine were taken from the area surrounding the scolex of all tapeworms found, fixed in PBS-paraformaldehyde and processed by histological techniques; 3µm sections were obtained and adhered to electrocharged slides. Antisense probes of DNA for the detection of mRNA were constructed according to published literature of IFN, IL-4, IL-5 and IL-13. In situ hybridization was performed with digoxigenin labeled oligonucleotides; alpha tubuline was used as a positive control and human papiloma virus (p16-2R) as a negative control. Immunological detection was obtained with antibodies against digoxigenin coupled to alkaline phosphatase or peroxidase. Kinetics of each cytokine was defined by counting the number of positive cells per 100 enterocytes. IFN was the first cytokine detected, followed by IL-13, since day 2 and 4 respectively. Then, IL-4 and IL-5 became apparent at the end of week one and continued positive for the following weeks. These data point to a shift from Th1 to Th2 cytokines, since no positive reaction for IFN was observed from week 2 onwards.

This Article Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Flisser, A. Articles by Garcia, F. PubMed Articles by Flisser, A. Articles by Garcia, F.