Data Supplement

Files in this Data Supplement:

• Supplemental Figure S1 (PDF, 46.7 Kb)
  - Effects of proteinase K in the generation of AVDCs. Three surface markers were studied, CD86, MHC-I and MHC-II. Red: effects of supernatant conditioned by NDV-infected DCs in one trans-well-chamber on naïve DCs in the other chamber. Green: effects of supernatant conditioned by NDV-infected DC subsequently digested with proteinase K in one transwell-chamber on naïve DCs in the other chamber. Blue: naïve DCs with unconditioned media in the other trans-well chamber. The data shown are representative of two replicate experiments using two different donors that showed similar results.
• Supplemental Figure S2 (PDF, 58.6 Kb)
  - Paracrine effects of virus-infected human epithelial fibroblasts on naïve DCs. Fibroblasts were seeded in the lower compartment of the trans-well system and were infected, (green), or not infected, (blue), with NDV to see if they were capable of generating the same maturation surface markers pattern as AVDCs (red). Fluorescence minus one control (FL -1) is shown in black. The data shown are representative of three different experiments using three different donors that showed similar results.
• Supplemental Figure S3 (PDF, 49.6 KB)
  - Effect of extended trans-well culturing on naïve DCs after 18, 24 and 48 hours, (red, blue and green respectively). FL-1 control is shown in black. The data shown are representative of three different experiments using three different donors that showed similar results.