Protein Kinase C Regulates Stability of the Peripheral Adhesion Ring Junction and Contributes to the Sensitivity of Target Cell Lysis by CTL

Data Supplement

Files in this Data Supplement:

• Movie 1 (MOV, 2.52 Mb)
  - CD4+ CTL form a peripheral ring junction that is more dynamic than that of CD8+ CTL.
  CD4+ CTL were added to bilayer containing ICAM-1 (300 molecles/μm²) and pMHC (500 molecules/μm²) and images were acquired from 5 to 10 minutes at 30-seconds intervals.
• Movie 2 (MOV, 3.81 Mb)
  - CD8+ CLT form a stable peripheral ring junction.
  CD8+ were added to bilayer containing ICAM-1 (300 molecles/μm2) and pMHC (500 molecules/μm2) and images were acquired from 5 to 10 minutes at 30-seconds intervals.