Data Supplement

Files in this Data Supplement:

• Video 1 (MOV, 413 Kb)
  - Disruption of CD28 localization at the IS following addition of anti-CD80. Movie corresponding to still images in Figure 2B.
• Video 2 (MOV, 369 Kb)
  - CD28 localization at the IS is not effected by the addition of the control, anti-class I mAb. Movie corresponding to still images in Figure 2C.
• Video 3 (MOV, 622 Kb)
  - Disruption of CD28 localization at the IS following addition of anti-class II. Movie corresponding to still images in Figure 3F.
• Video 4 (MOV, 440 Kb)
  - Colocalization of CD28 and PKCθ outside of the cSMAC in T cells expressing Y188F. Animated rotation of a 2-color overlay corresponding to the 3-D representation shown in the first panel of Fig 7F.
• Video 5 (MOV, 514 Kb)
  - Colocalization of CD28 and PKCθ outside of the cSMAC in T cells expressing Y188F. Animated rotation of a 2-color overlay corresponding to the 3-D representation shown in the second panel of Fig 7F.
• Video 6 (MOV, 310 Kb)
  - Colocalization of CD28 and PKCθ outside of the cSMAC in T cells expressing Y188F. Animated rotation of a 2-color overlay corresponding to the 3-D representation shown in the third panel of Fig 7F.