Correction
for Palazzo et al., J Immunol 181 (5) 3126-3136.
The Journal of Immunology, 2008,
181,
7428
Copyright © 2008 by The American Association of Immunologists, Inc.
Sodium-dependent glucose transporter-1 as a novel immunological player in the intestinal mucosa.
M. Palazzo,
S. Gariboldi,
L. Zanobbio,
S. Selleri,
G. F. Dusio,
V. Mauro,
A. Rossini,
A. Balsari and
Cr. Rumio
Palazzo, M., S. Gariboldi, L. Zanobbio, S. Selleri, G. F. Dusio, V. Mauro, A. Rossini, A. Balsari, and Cr. Rumio. 2008. Sodium-dependent glucose transporter-1 as a novel immunological player in the intestinal mucosa. J. Immunol. 181: 3126–3137
.
In Results, under the subhead titled SGLT-1 activation blocks NF-
B nuclear translocation induced by LPS and CpG-ODN, the corrected text in the fourth, fifth, and six sentences of the paragraph should read as follows: "In HT-29, LCC-18, and STC-1 cells stimulated with LPS or CpG-ODN, we observed activation of NF-B, i.e., translocation to the nucleus, whereas this activation was not detected in cells pretreated with D-glucose (Fig. 6A) or 3-OMG (data not shown). NF-B in the cytoplasm is complexed to members of the IB family of inhibitory proteins. Western blot analysis revealed degradation of IB when NF-B translocates to the nucleus upon LPS or CpG-ODN stimulation; degradation of IB in stimulated cells was inhibited by D-glucose (Fig. 6B) or 3-OMG (data not shown) pretreatment."
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FIGURE 6. A, Western blot analysis for NF-B in IEC nuclear extracts. HT29, LCC-18, and STC-1 cells were stimulated with LPS or CpG-ODN with or without D-glucose pretreatment. Nuclear extracts were analyzed by Western blotting with anti-NF-B p65 Ab. B, Western blot analysis for IB in IEC extracts. HT29, LCC-18, and STC-1 cells were stimulated with LPS or CpG-ODN with or without D-glucose pretreatment. Total protein extracts were analyzed by Western blotting with anti-IB Ab. C, Involvement of Akt signaling pathway. HT29 protein extracts, following LPS and/or glucose treatment, were analyzed by Western blotting with anti-phospho-Akt and anti-Akt Abs (C1). In addition, cells were stimulated with LPS, with or without D-glucose pretreatment, in the presence or absence of Akt inhibitor LY294002. Nuclear extracts were analyzed by Western blotting with anti-NF-B p65 Ab (C2). Untr, Untreated.
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In addition, changes have been made in Fig. 6 concerning the Western blots in B for LCC-18 and STC-1 and minor changes have also been made to the figure legend. Both the revised Fig. 6 and the revised legend are shown below.
