The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 2008, 180, 5582 -5592
Copyright © 2008 by The American Association of Immunologists, Inc.
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dunham, R. M.
Right arrow Articles by Silvestri, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunham, R. M.
Right arrow Articles by Silvestri, G.

CD127 and CD25 Expression Defines CD4+ T Cell Subsets That Are Differentially Depleted during HIV Infection 1

Richard M. Dunham*,{dagger}, Barbara Cervasi*, Jason M. Brenchley{ddagger}, Helmut Albrecht§, Amy Weintrob§, Beth Sumpter{dagger}, Jessica Engram*, Shari Gordon*,{dagger}, Nichole R. Klatt*,{dagger}, Ian Frank*, Donald L. Sodora, Daniel C. Douek{ddagger}, Mirko Paiardini* and Guido Silvestri2,*,{dagger}

* Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104; {dagger} Emory Vaccine Center, Emory University School of Medicine, and Yerkes National Primate Research Center, Atlanta, GA 30322; {ddagger} Human Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; § Infectious Diseases Clinic, Crawford-Long Hospital, Atlanta, GA 30308; and Seattle Biomedical Research Institute, Seattle, WA 98109


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Decreased CD4+ T cell counts are the best marker of disease progression during HIV infection. However, CD4+ T cells are heterogeneous in phenotype and function, and it is unknown how preferential depletion of specific CD4+ T cell subsets influences disease severity. CD4+ T cells can be classified into three subsets by the expression of receptors for two T cell-tropic cytokines, IL-2 (CD25) and IL-7 (CD127). The CD127+CD25low/– subset includes IL-2-producing naive and central memory T cells; the CD127–CD25– subset includes mainly effector T cells expressing perforin and IFN-{gamma}; and the CD127lowCD25high subset includes FoxP3-expressing regulatory T cells. Herein we investigated how the proportions of these T cell subsets are changed during HIV infection. When compared with healthy controls, HIV-infected patients show a relative increase in CD4+CD127–CD25– T cells that is related to an absolute decline of CD4+CD127+CD25low/– T cells. Interestingly, this expansion of CD4+CD127– T cells was not observed in naturally SIV-infected sooty mangabeys. The relative expansion of CD4+CD127–CD25– T cells correlated directly with the levels of total CD4+ T cell depletion and immune activation. CD4+CD127–CD25– T cells were not selectively resistant to HIV infection as levels of cell-associated virus were similar in all non-naive CD4+ T cell subsets. These data indicate that, during HIV infection, specific changes in the fraction of CD4+ T cells expressing CD25 and/or CD127 are associated with disease progression. Further studies will determine whether monitoring the three subsets of CD4+ T cells defined based on the expression of CD25 and CD127 should be used in the clinical management of HIV-infected individuals.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
HIV infection is associated with a progressive decline of CD4+ T cells and loss of immune function, which manifests clinically as an increased susceptibility to opportunistic infections and neoplasm. Early studies showed that HIV uses the CD4 molecule as an entry receptor and that infected CD4+ T cells are directly killed by the virus, providing a logical explanation for the progressive decline of these cells in HIV-infected individuals (1, 2). However, other studies have shown that, during chronic HIV infection, more CD4+ T cells die than can be accounted for by direct infection (3), and that, in fact, an increased death rate is observed even in nontarget cells, such as CD8+ T cells (4, 5, 6, 7, 8). This bystander loss of uninfected T cells is thought to be related to a state of generalized immune activation that is consistently associated with HIV infection (9). Based on these findings, it has been proposed that chronic immune activation plays a key role in the HIV-associated CD4+ T cell depletion (8, 10, 11, 12, 13). Consistent with this model is the finding that the expression of activation markers on T cells is a strong predictor of disease progression in HIV-infected individuals (14, 15).

During HIV infection, the dynamics of CD4+ T cells and their subsets are influenced by several factors, including the level of virus replication and the involvement of these cells in the immune response to HIV. Virus replication occurs preferentially in effector memory CD4+ T cells expressing the viral co-receptor CCR5 and thus results in a net loss of these cells that is particularly rapid and pronounced at the level of MALT (16, 17, 18). On the other hand, the HIV-associated chronic immune activation results in a constant recruitment of CD4+ T cells from the central memory and naive pools into the effector pool, thus creating a strain on the homeostatic mechanisms of CD4+ T cell maintenance (8, 10, 11, 12). As such, the net effect of HIV infection on the relative proportions of CD4+ naive (TN), 3 memory (TM), and effector (TE) T cells is far from understood and likely depends greatly on the stage of disease.

The level of HIV-associated chronic immune activation (and its related impact on CD4+ T cell homeostasis) is currently assessed by measuring the expression of markers of T cell maturation (CD45RA, CD62L, CCR7), activation (HLA-DR, CD38), and proliferation (Ki67) that are not, per se, suitable targets for immune-based interventions. In this study we sought to investigate the dynamics of the CD4+ T cell compartment following HIV infection by focusing on functional markers that could also serve as targets for immunomodulatory therapies. Expression of the receptors for IL-2 and IL-7 controls naive and memory T cell homeostasis, proliferation, and differentiation (19). IL-2 and IL-7 are members of a family of cytokines that utilize a common {gamma}-chain (CD132) in combination with high-affinity receptors: IL-2Rβ (CD122) and IL-2R{alpha} (CD25) for IL-2, and IL-7R{alpha} (CD127) for IL-7. IL-2/IL-2R{alpha} signaling is required for T cell development and to support Ag-specific T cell responses (19). Additionally, the role of IL-2 in promoting regulatory T cell (Treg) function is demonstrated by the finding that both IL-2–/– and IL-2R{alpha}–/– mice develop autoimmune and lymphoproliferative disorders (19). While the low-affinity IL-2R is constitutively expressed by T cells, TCR stimulation results in up-regulation of the {alpha}-chain and the assembly of the high-affinity heterotrimeric IL-2R complex. In contrast, Tregs constitutively express the high-affinity complex (CD25high). While several studies have shown abnormalities of the CD4+CD25+ Treg subset during chronic HIV infection (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30), it is still unclear to what extent these cells play a role in the immunopathogenesis of AIDS, particularly in terms of suppressing HIV-specific T cell responses and mitigating the HIV-associated chronic immune activation. Interestingly, the fraction of CD4+CD25+ T cells is dramatically increased after exogenous administration of IL-2 (31).

IL-7 signals are vital to T cell development, as IL-7–/– or IL-7R{alpha}–/– mice exhibit severe T cell deficiency (32, 33). The binding of IL-7 to its high-affinity receptor CD127 results in up-regulation of antiapoptotic molecules such as bcl-2 and enhancement of TCR-mediated signaling, thus priming the newly activated T cell for proliferation and IL-2 production (34, 35). After T cell activation by Ag or IL-7 binding, IL-7R{alpha} is lost from the cell surface (36) and can only be reexpressed by de novo protein synthesis (37). However, recent evidence indicates that during an acute T cell response to viral infection, a small number of effector phenotype T cells retain or reexpress IL-7R{alpha} and may possess a greater ability to become long-term memory than do the IL-7R{alpha}– cells (38, 39, 40, 41, 42). The surviving memory cells express IL-7R{alpha} and are somewhat dependent on IL-7 signals in concert with other cytokines for their homeostatic maintenance (19). During HIV infection and other lymphopenic conditions, IL-7 levels in plasma are increased (43, 44, 45), possibly due to increased production by stromal cells (46). The increased plasma concentration of IL-7 correlates with a sustained down-regulation of IL-7R{alpha} on TN and TM cells that is reversed once cells are removed from the IL-7-rich environment (37). Many CD8+ T cells, particularly those specific for HIV Ags, also lose expression of IL-7R{alpha} (47, 48) and exhibit an activated effector phenotype (49, 50). A general down-regulation of IL-7R{alpha} during HIV infection has also been observed in CD4+ T cells (37, 48, 49, 50, 51, 52).

Both IL-2 and IL-7 are currently being investigated as therapies to improve the immune system function in HIV-infected individuals (53, 54, 55, 56). These studies have shown that administration of IL-2 or IL-7 can dramatically increase the size of the CD4+ T cell pool and, in the case of IL-7, may rebalance the observed expansion of TE cells that occurs during HIV infection by expanding TN or TM cells (53, 54, 55, 56). The impact of IL-2 and IL-7 on CD4+ T cell survival and/or proliferation is contingent upon expression of CD25 or CD127, respectively. Herein we report a study of how HIV infection affects the relative proportions of three subsets of CD4+ T cells that are defined based on expression of CD127 and CD25 (57, 58, 59).


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Disclosures
 References
 
Patients

Peripheral blood was sampled from 56 asymptomatic HIV-infected patients with detectable virus replication from the Infectious Disease Clinic of Crawford Long Hospital (Atlanta, GA) and the Department of Infectious Diseases, Hospital of the University of Pennsylvania, as well as 25 healthy age-matched individuals. The main demographic and clinical features of the enrolled patients (age, sex, gender, viral load, CD+ T cell count, and status of antiretroviral therapy) are shown in Table I. These studies were approved by the Institutional Review Boards of Emory University and the University of Pennsylvania, and all participants gave informed consent.


View this table:
[in this window]
[in a new window]

  		Table I. Demographics and clinical data (including viral load, CD4+ T cell count, and treatment with antiretroviral therapy (ART)) of the HIV-infected individuals enrolled in the current study

 
Animals

Peripheral blood was sampled from 110 SIV-infected and 30 SIV-uninfected sooty mangabeys (Cercocebus atys) housed at the Yerkes National Primate Research Center (Atlanta, GA). All experiments were approved by the Institutional Animal Care and Use Committees of Emory University and the University of Pennsylvania.

Antibodies

Anti-CD127-PE was purchased from Beckman Coulter. Anti-CD3 (clone SP34-2)-Pacific Blue or Alexa700, anti-CD4 (clone L200 or SK3 or RPA-T4)-PerCP or PerCP-Cy5.5 or Alexa700, anti-CD8 (clone SK1 or RPA-T8)-PerCP or FITC or APC or PE-Cy7, anti-perforin (clone dG9)-FITC, anti-Ki67 (clone B56)-FITC, anti-CD25 (clone 2A3 or M-A251)-APC or FITC or APC-Cy7, anti-CD38-APC (HIT-2), anti-IL-2 (clone MQ1–17H12 or 5344.111)-FITC, anti-IFN-{gamma} (clone 25723.11)-APC, anti-CD62L (SK11)-FITC, anti-CD27 (clone L128)-FITC or PE, anti-CCR7 (clone 3D12)-PE-Cy7, anti-CD45RA (HI-100)-APC, anti-HLA-DR (L243)-APC or CyChrome, anti-CD28 (CD28.2)-CyChrome, anti-CCR5 (2D7/CCR5)-CyChrome and annexin V FITC were purchased from BD Biosciences. Anti-CD45RA (clone MEM-56)-Alexa405 was purchased from Caltag Laboratories. Anti-FoxP3 (PCH-101)-FITC was purchased from eBioscience.

Flow cytometry

PBMCs were isolated from whole blood by density gradient centrifugation using standard procedures. For intracellular Ags, cells were permeabilized using BD FACS-Perm2 (BD Biosciences) according to the manufacturer’s instructions. PBMCs were analyzed by four-to-eight-color fluorescent Ab staining. Flow cytometric acquisition and analysis were performed on at least 50,000 acquired events (gated on lymphocytes) on a FACSCalibur flow cytometer driven by the CellQuest software (BD Pharmingen) or an LSR II flow cytometer driven by FACSDiva software (BD Biosciences). Data analysis was performed using FlowJo software (Tree Star). Cell sorting experiments were performed on a FACSAria (BD Biosciences) after staining with fluorescent Abs specific for CD4, CD127, CD25, and CD45RA. Cells were sorted into RPMI 1640 containing 10% FBS, and the purity of sorted populations was always >98%. Absolute CD4+ T cell counts were calculated by multiplying the fraction of CD3+CD4+ cells within the forward light scatter-area/side light scatter-area-defined lymphocyte region by the total lymphocyte count derived from the complete blood count. This value was then used to calculate the absolute counts of the three studied CD4+ T cell subsets by multiplying the absolute CD4+ T cell count by the fractions of CD127+CD25low/–, CD127–CD25–, and CD127lowCD25high cells (gated as in Fig. 1A).


Figure 1
View larger version (15K):
[in this window]
[in a new window]

  		FIGURE 1. Expression of CD127 and CD25 defines three subsets of CD4+ T cells. A, Gating strategy for defining subsets of CD4+ T cells using CD127 and CD25. Plots shown are gated on lymphocytes, then CD3+, CD8–, and CD4+. B, Comparison of the average fraction of CD4+ T cells within each subset in HIV-infected (filled bars) and uninfected (open bars) individuals. ***, p < 0.001 (two-way ANOVA). C, Comparison of the absolute number of CD4+ T cells within each subset in HIV-infected individuals (filled bars) and uninfected (open bars) controls.

 
In vitro cytokine production

For intracellular cytokine staining, whole blood was centrifuged over a Cellgro (Mediatech) Lymphocyte Separation Medium density gradient, and mononuclear cells were harvested. These cells were then incubated for 6 h with PMA (10 ng/ml), the calcium ionophore A23187 (200 ng/ml), and monensin (10 nM) (all from Sigma-Sldrich). Surface staining was performed as described above. Cells were permeabilized using BD Cytofix/Cytoperm (BD Biosciences) according to the manufacturer’s instructions and stained for IL-2 or IFN-{gamma} at 4°C for 20 min.

Apoptosis

Lymphocytes were stained for the relevant surface markers and annexin V-FITC either immediately after isolation or after 24 or 48 h at 37°C in RPMI 1640 containing 10% FBS. For these experiments all blood samples were collected by venipuncture.

HIV cell-associated DNA content

Quantification of HIV-gag DNA in sorted CD4+ T cells was performed by quantitative real-time PCR by the 5'-nuclease (TaqMan) assay with an ABI7700 system (PerkinElmer) as previously described (60, 61). To quantify cell number in each reaction, quantitative PCR was performed simultaneously for albumin gene copy number as previously described (62). Standards were constructed for absolute quantification of gag and albumin copy number, and they were validated with sequential dilutions of 8E5 and Ach2 cell lysates that contain one copy of gag per cell. Duplicate reactions were run and template copies were calculated using ABI7700 software.

Statistical analysis

Statistical analysis was performed using GraphPad Prism v4.0c for Mac OS X. Matched pairwise comparisons were made using the Wilcoxon matched pairs test. One-factor multiple comparisons were computed with the Friedman test. Two-factor multiple comparisons were calculated using the two-way ANOVA. Correlations were performed using the Spearman rank correlation coefficient test. In all tests {alpha} was set to 0.05.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Disclosures
 References
 
Expression of CD127 and CD25 define three subsets of CD4+ T cells

Three subsets of CD4+ T cells are defined by the expression of IL-2R{alpha} (CD25) and IL-7R{alpha} (CD127): CD127+CD25low/–, CD127–CD25–, and CD127lowCD25high (Fig. 1A). In the healthy HIV-uninfected individuals enrolled in this study (n = 20), the majority (average 88.2 ± 2.87%) of CD4+ T cells were included in the CD127+CD25low/– subset, with the CD127lowCD25high and the CD127–CD25– subsets containing relatively few cells (average 7.45 ± 1.64% and 3.65 ± 2.67%, respectively) (Fig. 1B). When the relative proportions of the same three CD4+ T cell subsets were examined in HIV-infected individuals (n = 48, average CD4+ T cell count of 237 ± 184 cells/mm3), we observed a significant increase in the size of the CD127–CD25– subset (17.77 ± 13.0%) and a concomitant decline in the fraction of CD4+CD127+CD25low/– T cells (70.9 ± 15.79%) (Fig. 1B). The relative size of the CD127lowCD25high subset was only slightly elevated in HIV-infected individuals compared with controls (10.3 ± 7.27%).

In terms of absolute numbers of cells per cubic millimeter of blood, we observed a dramatic decline of the CD4+CD127+CD25low/– T cell subset in HIV-infected individuals (175 ± 148 cells/mm3) compared with controls (461 ± 318 cells/mm3) (p < 0.001, Fig. 1C). Similarly, the absolute number of CD4+CD127lowCD25high T cells was decreased in HIV-infected individuals when compared with controls, although in a less dramatic fashion (21 ± 15 vs 43 ± 30 cells/mm3, p = NS, Fig. 1C). In contrast, the absolute number of CD4+CD127–CD25– T cells was maintained, and, in fact, even slightly increased in HIV-infected patients (41 ± 45 vs 22 ± 14 cells/mm3, p = NS, Fig. 1C). These data indicate that the relative increase in size of the CD4+CD127–CD25– T cell subset associated with HIV infection is not due to an increased absolute number of CD127–CD25– cells but rather to a substantial and remarkably selective loss of CD127+CD25low/– cells (and, to a lesser extent, of CD127lowCD25high cells). These findings are in stark contrast to the changes observed in the expression of CD127 on CD8+ T cells during HIV infection, where a major expansion in the absolute number of CD8+CD127– T cells was associated with normal numbers of CD8+CD127+ cells (49).

Phenotypic and functional characteristics of the three subsets of CD4+ T cells identified by expression of CD25 and CD127

We next performed a detailed phenotypic and functional characterization of the three subsets of CD4+ T cells. We first used eight-color flow cytometry on PBMCs derived from both HIV-infected individuals and controls to assess the expression of CD45RA and CD62L and thus characterize the state of differentiation (i.e., naive vs central memory vs effector memory) of the CD4+ T cell subsets (63). As shown in Fig. 2A, we found that the CD127+CD25low/– subset is relatively equally divided between CD45RA+CD62L+ naive cells and CD45RA–CD62L+ central memory cells, that the CD127–CD25– subset includes the vast majority of the CD62L– effector memory cells, and that the CD127lowCD25high subset mostly expresses markers of central memory cells (CD45RA–CD62L+). Interestingly, while the phenotype of the CD127+CD25low/– and CD127lowCD25high subsets with respect to CD45RA and CD62L expression was similar between HIV-infected individuals and controls (Fig. 2A), the CD127–CD25– subset included a higher fraction of CD4+CD62L–CD45RA– and CD4+CD62L–CD45RA+ T cells in HIV-infected patients. Consistent with recent reports (57, 58, 59), >80% of the CD4+CD127lowCD25high T cells expressed FoxP3+ (not shown). This finding, together with the expression of a central memory phenotype (CD45RA–CD62L+) by these cells, prompted us to consider them as Tregs. Figure 2B shows the absolute numbers/mm3 of the three CD4+ T cell subsets included in the naive (CD45RA+CD62L+), central memory (CD45RA–CD62L+), and effector memory (CD62L–) populations.


Figure 2
View larger version (25K):
[in this window]
[in a new window]

  		FIGURE 2. CD127–CD25–CD4+ T cells express phenotypic and functional markers of effector T cells. Average fraction (A) and absolute number (B) of cells, within each of the three subsets of CD4+ T cells identified based on the expression of CD127 and CD25, that can be defined as naive (CD45RA+CD62L+), central memory (CD45RA–CD62L+), or effector memory (CD62L–) CD4+ T cells in HIV-infected (left) and uninfected (right) individuals. **, p < 0.01; ***, p < 0.001 (two-way ANOVA). C, Average proportion of activation marker expression by each CD127/CD25 CD4+ T cell subset in HIV-infected (left) and uninfected (right) individuals. **, p < 0.01; ***, p < 0.001 (one-way ANOVA comparing between CD127/CD25 subsets for each marker). D, Percentage of CD127+ (open bars) or CD127– (filled bars) CD4+ T cells that produced IFN-{gamma} or IL-2 in response to 4 h stimulation with 50 ng/ml PMA + 500 ng/ml ionomycin (A23187). *, p < 0.05; **, p < 0.01 (Wilcoxon matched pairs test). E, Average fraction of CD4+ T cell subsets binding annexin V directly ex vivo (baseline) and after 48 h incubation. All experiments were conducted in peripheral blood samples obtained by venipuncture. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (one-way ANOVA with Dunn’s multiple comparisons test).

 
We next measured the expression of markers of T cell activation within the subsets of CD4+ T cells defined by CD25/CD127 expression. Although all three subsets expressed each of the activation markers analyzed to some degree, the CD127–CD25– subset contained, in both HIV-infected patients and controls, the highest fraction of cells expressing the proliferation Ag Ki67, the activation marker HLA-DR, as well as lower levels of CD27 and CD28 (Fig. 2C). These findings are consistent with the previous observation that CD4+CD127–CD25– T cells are enriched in CD62L– cells (Fig. 2A). Importantly, note that HIV-infected patients did not show an increased fraction of cells expressing activation markers within any of the studied subsets, but rather a relative increase of the CD4+CD127–CD25– T cells that express the highest levels of activation markers in both HIV-infected patients and controls was shown.

To investigate the functional properties of the described CD4+ T cell subsets, we next measured the production of IL-2 and IFN-{gamma} by PBMCs isolated from HIV-infected individuals and controls using intracellular cytokine staining after stimulation with PMA/ionomycin (A23187). Because stimulation through the TCR and its downstream signaling pathways can modulate the expression of cytokine receptors, we conducted preliminary experiments to sequentially measure CD25 and CD127 expression over the course of 6 h of PMA/ionomycin stimulation. We found that while CD127 expression on CD4+ T cells remained constant, CD25 expression was up-regulated by 4 h of stimulation (not shown). We thus focused our assessment of cytokine production on CD4+CD127+ and CD4+CD127– T cells. As shown in Fig. 2D, CD4+ T cells from both HIV-infected individuals and controls show a particular pattern of cytokine production after 4 h of PMA + A23187 stimulation characterized by production predominantly of IL-2 by CD4+CD127+ T cells and IFN-{gamma} by CD4+CD127– T cells. The results of this experiment thus further support our previous phenotypic characterization of the CD4+CD127+CD25low/– T cell subset as including mainly naive and central memory cells and the CD4+CD127–CD25– T cell subset as including mainly effector memory or terminally differentiated cells.

We next measured the in vitro susceptibility of each of the CD4+ T cell subsets to both spontaneous and activation-induced apoptosis. To this end, we measured annexin V binding to lymphocytes derived from healthy donors and HIV-infected patients under two different experimental conditions: immediately postisolation (baseline apoptosis), and after 24 or 48 h incubation in the absence of any stimulation (spontaneous apoptosis). Fig. 2E shows that, while baseline apoptosis levels were slightly elevated in all CD4+ T cell subsets from HIV-infected individuals, the levels of spontaneous apoptosis were significantly higher for the CD127–CD25–, CD127lowCD25high, and CD127+CD25low/– CD45RO+ subsets as compared with the CD127+CD25low/– CD45RO– subset.

The relative expansion of CD4+CD127–CD25– T cells is correlated with markers of disease progression in HIV-infected individuals

During chronic HIV infection, the prevailing level of immune activation is a strong predictor of disease progression (64, 65). As the relative expansion of CD4+CD127–CD25– T cells in HIV-infected patients appears to reflect an increased fraction of effector memory CD4+ T cells, we next sought to determine whether any of the changes in the CD4+ T cell subsets that we observed during HIV infection correlated with markers of disease progression. We first observed a direct correlation between the fraction of CD4+CD127–CD25– (but not the CD4+CD127+CD25low/– T cells) and viral load (Fig. 3A), indicating that the relative shift toward the CD4+CD127–CD25– T cell subset is likely related to the prevailing level of virus replication. Interestingly, we found a significant inverse correlation between the proportion of CD4+CD127–CD25– T cells and the overall percentage of circulating CD4+ T cells, as well as a significant direct correlation between the proportion of CD4+CD127+CD25low/– T cells and the overall percentage of CD4+ T cells (Fig. 3B). As shown in Fig. 3C, we also observed a direct correlation between the fraction of CD4+CD127+CD25low/– T cells and the absolute CD4+ T cell count, while no correlation was observed between the percentage of CD4+CD127–CD25– T cells and the absolute CD4+ T cell count. These correlations suggest that the HIV-induced progressive decline of circulating CD4+ T cells is consistently associated with a relative expansion of CD4+CD127–CD25– T cells within the residual pool of CD4+ T cells.


Figure 3
View larger version (22K):
[in this window]
[in a new window]

  		FIGURE 3. The relative expansion of the CD127–CD25–CD4+ T cell subset is correlated with markers of disease progression. Correlations of the fractions of CD127–CD25– or CD127+CD25low/–CD4+ T cells with viral load (A), %CD4+ T cells (B), and absolute CD4+ T cell count (C) across a subset of HIV-infected individuals. Correlations were performed using the Spearman rank correlation coefficient test.

 
Given the known association between chronic T cell activation and disease progression in HIV-infected individuals (14, 64, 65), we also measured the relationship between the relative proportions of the three CD4+ T cell subsets and the level of immune activation, assessed as the fraction of CD8+ T cells that have lost the expression of CD28 (66) or CD127 (49). We observed a significant direct correlation between the fraction of CD4+CD127–CD25– T cells and both the fraction of CD8+CD28– T cells and CD8+CD127– T cells (p = 0.0279 and p = 0.0222, respectively, from Spearman rank correlation; data not shown). Taken together, these findings indicate that the relative expansion of CD4+CD127– T cells observed in HIV-infected individuals is correlated with both the level of CD4+ T cell depletion and the degree of in vivo T cell activation.

Nonpathogenic SIV infection of sooty mangabeys is not associated with changes in the proportions of CD4+ T cell subsets defined by CD127 and CD25

In a series of previous studies, we and others have shown that natural SIV infection of sooty mangabeys (SM), a nonhuman primate species from West Africa, is typically nonpathogenic and is associated with maintenance of CD4+ T cell homeostasis despite chronically high levels of virus replication (67, 68, 69, 70, 71). We thus proposed that, in naturally SIV-infected SMs, the presence of typically low levels of immune activation is instrumental in the preservation of peripheral CD4+ T cell counts and the maintenance of the disease-free state (72).

As such, we sought to determine whether natural SIV infection of SMs is associated with changes in the relative proportions of CD4+ T cell subsets defined based on CD127/CD25 expression. Fig. 4 shows averages of the CD4+ T cell subsets assessed in 110 SIV-infected and 30 uninfected SMs. No significant differences in the relative proportions of CD4+CD127+CD25low/–, CD4+ CD127lowCD25high, or CD4+CD127–CD25– T cell subsets were observed in SIV-infected as compared with uninfected SMs. Since natural SIV infection is typically characterized by a dissociation between virus replication and T cell activation, these findings further support the possibility that the imbalance of CD4+ T cell subsets observed in HIV-infected individuals is related to the chronic immune activation state rather than being a direct consequence of virus replication.


Figure 4
View larger version (7K):
[in this window]
[in a new window]

  		FIGURE 4. SIV infection of sooty mangabeys does not modulate the proportions of CD4+ T cell subsets defined by CD127 and CD25. Average fraction (left) and number per cubic millimeter of blood (right) of CD4+ T cells found in the CD127/CD25 subsets in peripheral blood of 110 SIV-infected (filled bars) and 30 uninfected (open bars) sooty mangabeys.

 
CD4+CD127+CD25– T cells are not preferentially infected during chronic HIV infection

The in vivo death of CD4+ T cells that occurs during chronic HIV infection is caused by two major, non-mutually exclusive mechanisms: 1) direct virus infection and 2) increased apoptosis of uninfected cells that is thought to be related to chronic immune activation (11, 12, 13). To directly assess the impact of direct virus infection on the described imbalance of CD4+ T cell subsets defined by CD127/CD25 expression, we measured the cell-associated HIV-DNA content in sorted CD4+ T cells. We first FACS-purified CD4+ T cells into the subsets defined above (CD127+CD25low/–, CD127–CD25–, CD127lowCD25high) and additionally subdivided the CD4+CD127+CD25low/– T cell subset using CD45RA to differentiate between TN (CD45RA+) and TM (CD45RA–) cells. This subdivision of the CD4+CD127+CD25low/– T cell subset was conducted to take into account that the presence of CD4+ TN cells, which are infrequently infected by HIV (60), within the CD4+CD127+ T cells would skew our assessment of the role of direct HIV infection in determining the relative expansion of CD4+CD127–CD25– T cells. In these experiments, HIV-gag copy number in each sorted subset was measured in cellular DNA extracts by quantitative real-time PCR using gag-specific primers as described (60, 61). As expected, the CD127+CD25low/–CD45RA+ naive CD4+ T cell subset contained the lowest median HIV-gag copies per cell (49 copies/cell, interquartile range of 10–185 copies/cell). Importantly, the remaining three subsets contained similarly high quantities of HIV-gag DNA (CD127+CD25low/–CD45RA– cells: 234 copies/cell, interquartile range of 100-1080 copies/cell; CD127–CD25– cells: 243 copies/cell, interquartile range of 103–647 copies/cell; and CD127lowCD25high cells: 404 copies/cell, interquartile range of 102–855 copies/cell) (Fig. 5A). These data indicate that differences between CD4+ T cell subsets with respect to their susceptibility to direct virus infection are unlikely to be the mechanism underlying the relative expansion of CD4+CD127–CD25– T cells that we found associated with chronic HIV infection.


Figure 5
View larger version (13K):
[in this window]
[in a new window]

  		FIGURE 5. CD127–CD25–CD4+ T cells are not preferentially infected during chronic HIV infection. A, Quantification of cell-associated HIV DNA content using quantitative, real-time PCR within the CD127/CD25 subsets of CD4+ T cells expressed as copies of HIV gag per 10,000 cells (normalized by copies of albumin). Friedman’s test was used to measure statistical significance. B, Comparison of the average fraction of each CD127/CD25 CD4+ T cell subset expressing CCR5 in HIV-infected (filled bars) and uninfected (open bars) individuals.

 
To further define the potential role of susceptibility to HIV infection of specific CD4+ T cell subsets, we next measured the expression of CCR5, the major co-receptor for HIV and a marker of T cell activation. As shown in Fig. 5B, we report that the expanded CD4+CD127–CD25– T cell subset contained the highest fraction of cells expressing CCR5 when compared with CD4+CD127+CD25low/– and CD4+CD127lowCD25high T cells. These findings are not unexpected, as CCR5 is preferentially expressed on effector memory CD4+ T cells (73) that are overrepresented in the subset of CD4+CD127–CD25– T cells (Fig. 2B). Together with the finding that CD4+CD127–CD25– T cells are not selectively spared from direct HIV infection, these CCR5 data suggest that the preferential loss of CD4+CD127+CD25low/– T cells reflects the ongoing chronic immune activation rather than an exquisite susceptibility to HIV-mediated killing. As such, these results further emphasize the pathogenic role of chronic T cell activation in determining the HIV-associated immune dysfunction.


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Disclosures
 References
 
Despite more than 25 years of intense study, the mechanisms by which HIV causes AIDS remain relatively poorly understood. The observation that HIV infection is followed by a progressive depletion of circulating CD4+ T cells has been traditionally interpreted as resulting from the selective infection and killing of CD4+ T cells (74). However, the HIV-associated immune dysfunction goes above and beyond the numeric loss of CD4+ T cells, as it involves functional abnormalities of the CD4+ T cells that remain as well as impaired function of many other immune cell types (75, 76, 77, 78). While some of the non-CD4+ T cell dysfunction has been proposed to be a secondary consequence of the overall loss of CD4+ T cell-mediated "help", it has become apparent that both the loss of CD4+ T cells and the generalized immune abnormalities are related to a state of chronic immune activation (8, 10, 11, 12). Crucial evidence in favor of the pathogenic role of the HIV-associated immune activation includes 1) the observation that T cell activation is a better predictor of disease progression than is virus replication (14, 64, 65), and 2) the discovery that SIV infection of natural hosts is typically nonpathogenic despite high-level virus replication in the context of minimal immune activation (69, 70, 71).

In this study, we sought to address the pathogenic role of functional abnormalities within the pool of residual CD4+ T cells and/or changes in the relative proportions of specific CD4+ T cell subsets in HIV-infected patients. We thus assessed the HIV-associated qualitative changes within the CD4+ T cell compartment by applying a recently proposed phenotypic classification of CD4+ T cell subsets based on the expression of CD127 and CD25 (57, 58, 59). According to these studies, circulating CD4+ T cells can be divided into three phenotypically and functionally distinct subsets: 1) CD127+CD25low/– cells that include naive and central memory CD4+ T cells, 2) CD127–CD25– cells that show characteristics of effector memory CD4+ T cells, and 3) CD127lowCD25high cells that express markers of regulatory T cells. Importantly, this classification may prove clinically useful as expression of CD127 and CD25 is linked to specific CD4+ T cell functions (i.e., responsiveness to IL-7 and IL-2, respectively) that may be targeted as part of immune-based interventions for HIV infection.

In this study, we found that during HIV infection the relative proportion (but not absolute number) of the CD4+CD127–CD25– subset was significantly and consistently expanded, due to a selective absolute loss of the CD127+CD25low/– subset. These results indicate that, within the overall CD4+ T cell pool, the well-known increase in the fraction of cells showing signs of immune activation (such as up-regulation of HLA-DR and loss of CD28 expression (66, 79)) is not due to a generalized modification of the levels of these markers on all CD4+ T cell subsets, but is rather due to the CD4+ T cell population being depleted of TN and TM cells (80) that rarely express markers of activation irrespective of HIV infection. Importantly, we found that the relative expansion of the CD4+CD127–CD25– T cell subset correlates with markers of disease progression such as the depletion of CD4+ T cells and the level of immune activation, suggesting a role for the described changes in the proportions of the CD4+ T cell subsets in the pathogenesis of HIV infection and AIDS. The current study examined only peripheral blood-derived CD4+ T cells. Although studies of other tissues are difficult to perform in humans, it will be important, in future work, to determine whether a similar increase of CD4+CD127– T cells is present in lymph nodes (where most CD4+ TN and TM cells reside).

While other studies have suggested that chronic HIV infection is associated with dysfunction of the Treg subset, either in the direction of an increased (21, 24, 25, 26, 30) or reduced (23, 27) ability to regulate T cell responses, our study failed to reveal any significant change in the relative proportion of CD4+CD127lowCD25high T cells in HIV-infected individuals compared with uninfected controls. As we have not investigated the Treg function in detail, our findings are compatible with the possibilities that Treg function is affected by HIV infection in a way that does not involve major changes in the proportion of these cells within the circulating CD4+ T cell compartment, or that, in fact, changes in Treg activity are not a key factor in AIDS pathogenesis.

The association between the relative expansion of CD4+CD127– T cells and both decreased CD4+ T cell levels and increased immune activation can be explained in several ways. First, it could be hypothesized that CD4+CD127–CD25– T cells are selectively spared by the virus, perhaps as a consequence of lower CCR5 expression. However, the data presented here show clearly that the level of cell-associated virus is similar in this subset of CD4+ T cells when compared with other CD4+ TM cell subsets, and that CCR5 expression on CD4+CD127–CD25– T cells is, in fact, higher than on other subsets of CD4+ T cells. Another potential interpretation is that CD4+CD127–CD25– T cells are intrinsically less prone to the apoptosis of bystander, uninfected T cells that is typically associated with HIV infection (81, 82). In this study, the direct measurement of ex vivo spontaneous apoptotic cell death did not indicate any preferential apoptosis of CD4+CD127+ T cells. Note, however, that this work has not ruled out a differential susceptibility of CD4+CD127+CD25– T cells to Fas-mediated apoptosis, a possibility suggested by the observation that IL-7 increases susceptibility to Fas-mediated apoptosis (83). A further explanation for the loss of IL-7R{alpha} expression on CD4+ T cells that is associated with HIV infection is that increased levels of circulating IL-7 result in active down-regulation of CD127 expression on a subset of CD4+ T cells (37). While we have not directly addressed this possibility in the current study, it should be noted that postligand binding down-regulation of a receptor (as occurs for CD127 on IL-7-exposed TN and TM cells) is a relatively transient phenomenon that is conceptually and biologically distinct from the permanent loss of expression of a given receptor that occurs as a result of cellular differentiation (as occurs for CD127 on TE) (37). Consistent with this view are recent reports showing a significant increase in the proportion of CD4+CD127– and CD8+CD127– T cells in hepatitis C virus infection in absence of any significant change in plasma IL-7 concentration (84). A final and perhaps more likely possibility is that the expansion of CD4+CD127–CD25– T cells reflects, both as a determinant and as a consequence, the HIV-associated chronic immune activation. In this perspective, it is conceivable that the CD4+CD127–CD25– T cells that show features of activated effectors, including production of IFN-{gamma} but not IL-2 (as demonstrated here), could be key promoters of the overall level of immune activation and thus contribute to AIDS pathogenesis. The proportional expansion of these CD4+CD127–CD25– T cells is in turn promoted by the level of immune activation and its detrimental effects on the development of naive T cells in the thymus and the homeostasis of memory T cells in the periphery. Taken as a whole, these results demonstrate that apoptosis and direct HIV infection cannot account for the imbalance of the CD4+ T cell compartment with respect to subsets of cells expressing CD127, highlighting the role of immune activation in driving HIV pathogenesis.

From a clinical point of view, the definition of subsets of CD4+ T cells that are selectively preserved or depleted during HIV infection may provide some additional predictive value for when and how CD4+ T cell depletion leads to full-blown AIDS. Clinicians involved in the management and therapy of HIV-infected individuals have long known that similarly low absolute CD4+ T cell counts can be associated with highly variable times to progression to AIDS. In this context, our current findings suggest that the relative expansion of CD4+CD127–CD25– T cells represents an additional marker of immune dysfunction during HIV infection. Note that CD4+CD127–CD25– T cells do not include naive or central memory cells, thus suggesting that a relative expansion of these CD4+CD127– cells has virtually no role in protecting from new or recall antigenic challenges. This fact, together with the basic premise that CD4+CD127– T cells are resistant to IL-7-mediated mechanisms of immunological homeostasis, provides a logical explanation for the hypothesis that the relative expansion of CD4+CD127– T cells is an index of CD4+ T cell dysfunction in HIV-infected individuals. As such, measurement of the fraction of CD4+CD127– T cells may complement the information provided by CD4+ T cell counts in assessing the risk of developing AIDS. Consistent with the hypothesis that the relative increase of the CD4+CD127–CD25– T cell subset is a marker of disease progression during HIV infection is the observation that, in naturally SIV-infected SMs (in which virus replication is associated with neither immunodeficiency nor generalized immune activation), the level of CD4+CD127–CD25– T cells is comparable to that observed in uninfected animals. While a larger prospective study is needed to confirm the clinical utility of measuring CD127 and CD25 expression on CD4+ T cells, our current work provides the rationale for further exploring the possibility that monitoring the fraction of specific CD4+ T cell subsets could be helpful to identify patients at high risk to progress to AIDS and who would thus be candidates for more aggressive therapeutic and/or prophylactic regimens.

Finally, the demonstration of a selective increase in the fraction of CD4+ T cells that do not express CD127 provides some indirect hints as to the potential role of IL-7 as an immunological therapy for HIV infection. The limitations of the currently available antiviral regimens (cost, side effects, inability to eradicate infection), as well as increasing evidence that immune-mediated mechanisms (i.e., chronic immune activation) play an active role in AIDS pathogenesis, provide the rationale for exploring new therapeutic strategies aimed at directly correcting the HIV-induced immune dysfunction. IL-7 appears particularly promising due to its potential ability to increase the survival and expansion of the subsets of mature T cells expressing its high-affinity receptor, CD127 (i.e., naive and central memory). Additionally, IL-7 has the ability to enhance T cell development at the level of bone marrow and thymus, thus leading to increased production of naive T cells (85). The observation that, in both HIV-infected individuals and SIV-infected rhesus macaques, IL-7 treatment results in an expansion of naive and memory CD4+ T cells gives further foundation for this intervention (56, 86, 87, 88, 89, 90). In this context, measurement of CD127 expression on CD4+ T cells might prove useful to predict the type of immunological response to IL-7, and thus identify HIV-infected patients who would be most likely to benefit from this treatment.


   Acknowledgments
 
We thank Mike Hulsey for flow cytometry support, Dr. Ann Chahroudi for critical review of this manuscript, and Antoine Almonte for helpful discussions.


   Disclosures
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
References
 
The authors have no financial conflicts of interest.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 This work was funded by Grants R01 AI52755 and R01 AI66998 from the National Institute of Allergy and Infectious Diseases (to G.S.) and from Grant RR-00165 from the National Center for Research Resources (to the Yerkes National Primate Research Center). Back

2 Address correspondence and reprint requests to Dr. Guido Silvestri, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 705 Stellar Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104. E-mail address: gsilvest{at}mail.med.upenn.edu Back

3 Abbreviations used in this paper: TN, naive T; TM, memory T; TE, effector T; Treg, regulatory T cell; SM, sooty mangabey. Back

Received for publication July 7, 2007. Accepted for publication February 8, 2008.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 

  1. Ho, D. D., A. U. Neumann, A. S. Perelson, W. Chen, J. M. Leonard, M. Markowitz. 1995. Rapid turnover of plasma virions and CD4 lymphocytes in HIV-1 infection. Nature 373: 123-126. [Medline]
  2. Wei, X., S. K. Ghosh, M. E. Taylor, V. A. Johnson, E. A. Emini, P. Deutsch, J. D. Lifson, S. Bonhoeffer, M. A. Nowak, B. H. Hahn, et al 1995. Viral dynamics in human immunodeficiency virus type 1 infection. Nature 373: 117-122. [Medline]
  3. Haase, A. T.. 1999. Population biology of HIV-1 infection: viral and CD4+ T cell demographics and dynamics in lymphatic tissues. Annu. Rev. Immunol. 17: 625-656. [Medline]
  4. Mohri, H., S. Bonhoeffer, S. Monard, A. S. Perelson, D. D. Ho. 1998. Rapid turnover of T lymphocytes in SIV-infected rhesus macaques. Science 279: 1223-1227. [Abstract/Free Full Text]
  5. Rosenzweig, M., M. A. DeMaria, D. M. Harper, S. Friedrich, R. K. Jain, R. P. Johnson. 1998. Increased rates of CD4+ and CD8+ T lymphocyte turnover in simian immunodeficiency virus-infected macaques. Proc. Natl. Acad. Sci. USA. 95: 6388-6393. [Abstract/Free Full Text]
  6. Hellerstein, M., M. B. Hanley, D. Cesar, S. Siler, C. Papageorgopoulos, E. Wieder, D. Schmidt, R. Hoh, R. Neese, D. Macallan, et al 1999. Directly measured kinetics of circulating T lymphocytes in normal and HIV-1-infected humans. Nat. Med. 5: 83-89. [Medline]
  7. Hellerstein, M. K., R. A. Hoh, M. B. Hanley, D. Cesar, D. Lee, R. A. Neese, J. M. McCune. 2003. Subpopulations of long-lived and short-lived T cells in advanced HIV-1 infection. J. Clin. Invest. 112: 956-966. [Medline]
  8. Silvestri, G., M. B. Feinberg. 2003. Turnover of lymphocytes and conceptual paradigms in HIV infection. J. Clin. Invest. 112: 821-824. [Medline]
  9. Hazenberg, M. D., J. W. Stuart, S. A. Otto, J. C. Borleffs, C. A. Boucher, R. J. de Boer, F. Miedema, D. Hamann. 2000. T-cell division in human immunodeficiency virus (HIV)-1 infection is mainly due to immune activation: a longitudinal analysis in patients before and during highly active antiretroviral therapy (HAART). Blood 95: 249-255. [Abstract/Free Full Text]
  10. Hazenberg, M. D., D. Hamann, H. Schuitemaker, F. Miedema. 2000. T cell depletion in HIV-1 infection: how CD4+ T cells go out of stock. Nat. Immunol. 1: 285-289. [Medline]
  11. McCune, J. M.. 2001. The dynamics of CD4+ T-cell depletion in HIV disease. Nature 410: 974-979. [Medline]
  12. Grossman, Z., M. Meier-Schellersheim, A. E. Sousa, R. M. Victorino, W. E. Paul. 2002. CD4+ T-cell depletion in HIV infection: are we closer to understanding the cause?. Nat. Med. 8: 319-323. [Medline]
  13. Douek, D. C., L. J. Picker, R. A. Koup. 2003. T cell dynamics in HIV-1 infection. Annu. Rev. Immunol. 21: 265-304. [Medline]
  14. Giorgi, J. V., L. E. Hultin, J. A. McKeating, T. D. Johnson, B. Owens, L. P. Jacobson, R. Shih, J. Lewis, D. J. Wiley, J. P. Phair, et al 1999. Shorter survival in advanced human immunodeficiency virus type 1 infection is more closely associated with T lymphocyte activation than with plasma virus burden or virus chemokine coreceptor usage. J. Infect. Dis. 179: 859-870. [Medline]
  15. Sousa, A. E., J. Carneiro, M. Meier-Schellersheim, Z. Grossman, R. M. Victorino. 2002. CD4 T cell depletion is linked directly to immune activation in the pathogenesis of HIV-1 and HIV-2 but only indirectly to the viral load. J. Immunol. 169: 3400-3406. [Abstract/Free Full Text]
  16. Guadalupe, M., E. Reay, S. Sankaran, T. Prindiville, J. Flamm, A. McNeil, S. Dandekar. 2003. Severe CD4+ T-cell depletion in gut lymphoid tissue during primary human immunodeficiency virus type 1 infection and substantial delay in restoration following highly active antiretroviral therapy. J. Virol. 77: 11708-11717. [Abstract/Free Full Text]
  17. Brenchley, J. M., T. W. Schacker, L. E. Ruff, D. A. Price, J. H. Taylor, G. J. Beilman, P. L. Nguyen, A. Khoruts, M. Larson, A. T. Haase, D. C. Douek. 2004. CD4+ T cell depletion during all stages of HIV disease occurs predominantly in the gastrointestinal tract. J. Exp. Med. 200: 749-759. [Abstract/Free Full Text]
  18. Mehandru, S., M. A. Poles, K. Tenner-Racz, A. Horowitz, A. Hurley, C. Hogan, D. Boden, P. Racz, M. Markowitz. 2004. Primary HIV-1 infection is associated with preferential depletion of CD4+ T lymphocytes from effector sites in the gastrointestinal tract. J. Exp. Med. 200: 761-770. [Abstract/Free Full Text]
  19. Ma, A., R. Koka, P. Burkett. 2006. Diverse functions of IL-2, IL-15, and IL-7 in lymphoid homeostasis. Annu. Rev. Immunol. 24: 657-679. [Medline]
  20. Kinter, A. L., R. Horak, M. Sion, L. Riggin, J. McNally, Y. Lin, R. Jackson, A. O’Shea, G. Roby, C. Kovacs, et al 2007. CD25+ regulatory T cells isolated from HIV-infected individuals suppress the cytolytic and nonlytic antiviral activity of HIV-specific CD8+ T cells in vitro. AIDS Res. Hum. Retroviruses 23: 438-450. [Medline]
  21. Kinter, A., J. McNally, L. Riggin, R. Jackson, G. Roby, A. S. Fauci. 2007. Suppression of HIV-specific T cell activity by lymph node CD25+ regulatory T cells from HIV-infected individuals. Proc. Natl. Acad. Sci. USA 104: 3390-3395. [Abstract/Free Full Text]
  22. Baker, C. A., R. Clark, F. Ventura, N. G. Jones, D. Guzman, D. R. Bangsberg, H. Cao. 2007. Peripheral CD4 loss of regulatory T cells is associated with persistent viraemia in chronic HIV infection. Clin. Exp. Immunol. 147: 533-539. [Medline]
  23. Kinter, A. L., M. Hennessey, A. Bell, S. Kern, Y. Lin, M. Daucher, M. Planta, M. McGlaughlin, R. Jackson, S. F. Ziegler, A. S. Fauci. 2004. CD25+CD4+ regulatory T cells from the peripheral blood of asymptomatic HIV-infected individuals regulate CD4+ and CD8+ HIV-specific T cell immune responses in vitro and are associated with favorable clinical markers of disease status. J. Exp. Med. 200: 331-343. [Abstract/Free Full Text]
  24. Nilsson, J., A. Boasso, P. A. Velilla, R. Zhang, M. Vaccari, G. Franchini, G. M. Shearer, J. Andersson, C. Chougnet. 2006. HIV-1-driven regulatory T-cell accumulation in lymphoid tissues is associated with disease progression in HIV/AIDS. Blood 108: 3808-3817. [Abstract/Free Full Text]
  25. Andersson, J., A. Boasso, J. Nilsson, R. Zhang, N. J. Shire, S. Lindback, G. M. Shearer, C. A. Chougnet. 2005. The prevalence of regulatory T cells in lymphoid tissue is correlated with viral load in HIV-infected patients. J. Immunol. 174: 3143-3147. [Abstract/Free Full Text]
  26. Eggena, M. P., B. Barugahare, N. Jones, M. Okello, S. Mutalya, C. Kityo, P. Mugyenyi, H. Cao. 2005. Depletion of regulatory T cells in HIV infection is associated with immune activation. J. Immunol. 174: 4407-4414. [Abstract/Free Full Text]
  27. Tsunemi, S., T. Iwasaki, T. Imado, S. Higasa, E. Kakishita, T. Shirasaka, H. Sano. 2005. Relationship of CD4+CD25+ regulatory T cells to immune status in HIV-infected patients. AIDS 19: 879-886. [Medline]
  28. Weiss, L., V. Donkova-Petrini, L. Caccavelli, M. Balbo, C. Carbonneil, Y. Levy. 2004. Human immunodeficiency virus-driven expansion of CD4+CD25+ regulatory T cells, which suppress HIV-specific CD4 T-cell responses in HIV-infected patients. Blood 104: 3249-3256. [Abstract/Free Full Text]
  29. Montes, M., D. E. Lewis, C. Sanchez, D. L. de Castilla, E. A. Graviss, C. Seas, E. Gotuzzo, A. C. White, Jr. 2006. Foxp3+ regulatory T cells in antiretroviral-naive HIV patients. AIDS 20: 1669-1671. [Medline]
  30. Epple, H. J., C. Loddenkemper, D. Kunkel, H. Troger, J. Maul, V. Moos, E. Berg, R. Ullrich, J. D. Schulzke, H. Stein, et al 2006. Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART. Blood 108: 3072-3078. [Abstract/Free Full Text]
  31. Sereti, I., H. C. Lane. 2001. Immunopathogenesis of human immunodeficiency virus: implications for immune-based therapies. Clin. Infect. Dis. 32: 1738-1755. [Medline]
  32. von Freeden-Jeffry, U., P. Vieira, L. A. Lucian, T. McNeil, S. E. Burdach, R. Murray. 1995. Lymphopenia in interleukin (IL)-7 gene-deleted mice identifies IL-7 as a nonredundant cytokine. J. Exp. Med. 181: 1519-1526. [Abstract/Free Full Text]
  33. Peschon, J. J., P. J. Morrissey, K. H. Grabstein, F. J. Ramsdell, E. Maraskovsky, B. C. Gliniak, L. S. Park, S. F. Ziegler, D. E. Williams, C. B. Ware, et al 1994. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J. Exp. Med. 180: 1955-1960. [Abstract/Free Full Text]
  34. Fry, T. J., C. L. Mackall. 2001. Interleukin-7: master regulator of peripheral T-cell homeostasis?. Trends Immunol. 22: 564-571. [Medline]
  35. Fry, T. J., C. L. Mackall. 2005. The many faces of IL-7: from lymphopoiesis to peripheral T cell maintenance. J. Immunol. 174: 6571-6576. [Abstract/Free Full Text]
  36. Park, J. H., Q. Yu, B. Erman, J. S. Appelbaum, D. Montoya-Durango, H. L. Grimes, A. Singer. 2004. Suppression of IL7Ralpha transcription by IL-7 and other prosurvival cytokines: a novel mechanism for maximizing IL-7-dependent T cell survival. Immunity 21: 289-302. [Medline]
  37. Sasson, S. C., J. J. Zaunders, G. Zanetti, E. M. King, K. M. Merlin, D. E. Smith, K. K. Stanley, D. A. Cooper, A. D. Kelleher. 2006. Increased plasma interleukin-7 level correlates with decreased CD127 and increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection. J. Infect. Dis. 193: 505-514. [Medline]
  38. Kaech, S. M., J. T. Tan, E. J. Wherry, B. T. Konieczny, C. D. Surh, R. Ahmed. 2003. Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells. Nat. Immunol. 4: 1191-1198. [Medline]
  39. Schluns, K. S., W. C. Kieper, S. C. Jameson, L. Lefrançois. 2000. Interleukin-7 mediates the homeostasis of naive and memory CD8 T cells in vivo. Nat. Immunol. 1: 426-432. [Medline]
  40. Tan, J. T., E. Dudl, E. LeRoy, R. Murray, J. Sprent, K. I. Weinberg, C. D. Surh. 2001. IL-7 is critical for homeostatic proliferation and survival of naive T cells. Proc. Natl. Acad. Sci. USA 98: 8732-8737. [Abstract/Free Full Text]
  41. Fuller, M. J., D. A. Hildeman, S. Sabbaj, D. E. Gaddis, A. E. Tebo, L. Shang, P. A. Goepfert, A. J. Zajac. 2005. Cutting edge: Emergence of CD127high functionally competent memory T cells is compromised by high viral loads and inadequate T cell help. J. Immunol. 174: 5926-5930. [Abstract/Free Full Text]
  42. Lang, K. S., M. Recher, A. A. Navarini, N. L. Harris, M. Lohning, T. Junt, H. C. Probst, H. Hengartner, R. M. Zinkernagel. 2005. Inverse correlation between IL-7 receptor expression and CD8 T cell exhaustion during persistent antigen stimulation. Eur. J. Immunol. 35: 738-745. [Medline]
  43. Mastroianni, C. M., G. Forcina, G. d’Ettorre, M. Lichtner, F. Mengoni, C. D’Agostino, V. Vullo. 2001. Circulating levels of interleukin-7 in antiretroviral-naive and highly active antiretroviral therapy-treated HIV-infected patients. HIV Clin. Trials 2: 108-112. [Medline]
  44. Llano, A., J. Barretina, A. Gutierrez, J. Blanco, C. Cabrera, B. Clotet, J. A. Este. 2001. Interleukin-7 in plasma correlates with CD4 T-cell depletion and may be associated with emergence of syncytium-inducing variants in human immunodeficiency virus type 1-positive individuals. J. Virol. 75: 10319-10325. [Abstract/Free Full Text]
  45. Beq, S., J. F. Delfraissy, J. Theze. 2004. Interleukin-7 (IL-7): immune function, involvement in the pathogenesis of HIV infection and therapeutic potential. Eur. Cytokine Network 15: 279-289. [Medline]
  46. Napolitano, L. A., R. M. Grant, S. G. Deeks, D. Schmidt, S. C. De Rosa, L. A. Herzenberg, B. G. Herndier, J. Andersson, J. M. McCune. 2001. Increased production of IL-7 accompanies HIV-1-mediated T-cell depletion: implications for T-cell homeostasis. Nat. Med. 7: 73-79. [Medline]
  47. Carini, C., M. F. McLane, K. H. Mayer, M. Essex. 1994. Dysregulation of interleukin-7 receptor may generate loss of cytotoxic T cell response in human immunodeficiency virus type 1 infection. Eur. J. Immunol. 24: 2927-2934. [Medline]
  48. Vingerhoets, J., E. Bisalinkumi, G. Penne, R. Colebunders, E. Bosmans, L. Kestens, G. Vanham. 1998. Altered receptor expression and decreased sensitivity of T-cells to the stimulatory cytokines IL-2, IL-7 and IL-12 in HIV infection. Immunol. Lett. 61: 53-61. [Medline]
  49. Paiardini, M., B. Cervasi, H. Albrecht, A. Muthukumar, R. Dunham, S. Gordon, H. Radziewicz, G. Piedimonte, M. Magnani, M. Montroni, et al 2005. Loss of CD127 expression defines an expansion of effector CD8+ T cells in HIV-infected individuals. J. Immunol. 174: 2900-2909. [Abstract/Free Full Text]
  50. Colle, J. H., J. L. Moreau, A. Fontanet, O. Lambotte, M. Joussemet, J. F. Delfraissy, J. Theze. 2006. CD127 expression and regulation are altered in the memory CD8 T cells of HIV-infected patients—reversal by highly active anti-retroviral therapy (HAART). Clin. Exp. Immunol. 143: 398-403. [Medline]
  51. Koesters, S. A., J. B. Alimonti, C. Wachihi, L. Matu, O. Anzala, J. Kimani, J. E. Embree, F. A. Plummer, K. R. Fowke. 2006. IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation. Eur. J. Immunol. 36: 336-344. [Medline]
  52. Rethi, B., C. Fluur, A. Atlas, M. Krzyzowska, F. Mowafi, S. Grutzmeier, A. De Milito, R. Bellocco, K. I. Falk, E. Rajnavolgyi, F. Chiodi. 2005. Loss of IL-7R{alpha} is associated with CD4 T-cell depletion, high interleukin-7 levels and CD28 down-regulation in HIV infected patients. AIDS 19: 2077-2086. [Medline]
  53. Kovacs, J. A., R. A. Lempicki, I. A. Sidorov, J. W. Adelsberger, I. Sereti, W. Sachau, G. Kelly, J. A. Metcalf, R. T. Davey, Jr, J. Falloon, et al 2005. Induction of prolonged survival of CD4+ T lymphocytes by intermittent IL-2 therapy in HIV-infected patients. J. Clin. Invest. 115: 2139-2148. [Medline]
  54. Sereti, I., K. B. Anthony, H. Martinez-Wilson, R. Lempicki, J. Adelsberger, J. A. Metcalf, C. W. Hallahan, D. Follmann, R. T. Davey, J. A. Kovacs, H. C. Lane. 2004. IL-2-induced CD4+ T-cell expansion in HIV-infected patients is associated with long-term decreases in T-cell proliferation. Blood 104: 775-780. [Abstract/Free Full Text]
  55. Sereti, I., E. Aga, J. Spritzler, A. Landay, S. Pahwa, M. Fischl, D. Asmuth, A. Tenorio, R. Buffet, and M. M. Lederman. 2007. rhIL-7 in HIV-1-infected subjects with CD4 T-cell count >100cells/uL and viral load <50,000 copies/mL: results from a randomized, placebo-controlled, double-blinded study (ACTG5214). In 14th Conference on Retroviruses and Opportunistic Infections, February 25–28, 2007, Los Angeles, CA.
  56. Levy, Y., L. Weiss, J. P. Viard, J. Molina, C. Goujard, F. Boue, S. Delluc, C. Rouzioux, M. Morre, and J. F. Delfraissy. 2007. Repeated r-hIL-7 doses improve T-cell recovery in HIV-1-infected patients enrolled in a phase I/II multicentric study. In 14th Conference on Retroviruses and Opportunistic Infections, February 25–28, 2007, Los Angeles, CA.
  57. Liu, W., A. L. Putnam, Z. Xu-Yu, G. L. Szot, M. R. Lee, S. Zhu, P. A. Gottlieb, P. Kapranov, T. R. Gingeras, B. Fazekas de St Groth, et al 2006. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ Treg cells. J. Exp. Med. 203: 1701-1711. [Abstract/Free Full Text]
  58. Seddiki, N., B. Santner-Nanan, J. Martinson, J. Zaunders, S. Sasson, A. Landay, M. Solomon, W. Selby, S. I. Alexander, R. Nanan, et al 2006. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700. [Abstract/Free Full Text]
  59. Hartigan-O’Connor, D. J., C. Poon, E. Sinclair, J. M. McCune. 2007. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J. Immunol. Methods 319: 41-52. [Medline]
  60. Brenchley, J. M., B. J. Hill, D. R. Ambrozak, D. A. Price, F. J. Guenaga, J. P. Casazza, J. Kuruppu, J. Yazdani, S. A. Migueles, M. Connors, et al 2004. T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesis. J. Virol. 78: 1160-1168. [Abstract/Free Full Text]
  61. Douek, D. C., M. R. Betts, J. M. Brenchley, B. J. Hill, D. R. Ambrozak, K. L. Ngai, N. J. Karandikar, J. P. Casazza, R. A. Koup. 2002. A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape. J. Immunol. 168: 3099-3104. [Abstract/Free Full Text]
  62. Douek, D. C., J. M. Brenchley, M. R. Betts, D. R. Ambrozak, B. J. Hill, Y. Okamoto, J. P. Casazza, J. Kuruppu, K. Kunstman, S. Wolinsky, et al 2002. HIV preferentially infects HIV-specific CD4+ T cells. Nature 417: 95-98. [Medline]
  63. Sallusto, F., J. Geginat, A. Lanzavecchia. 2004. Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu. Rev. Immunol. 22: 745-763. [Medline]
  64. Giorgi, J. V., R. H. Lyles, J. L. Matud, T. E. Yamashita, J. W. Mellors, L. E. Hultin, B. D. Jamieson, J. B. Margolick, C. R. Rinaldo, Jr, J. P. Phair, R. Detels. 2002. Predictive value of immunologic and virologic markers after long or short duration of HIV-1 infection. J. Acquir. Immune Defic. Syndr. 29: 346-355. [Medline]
  65. Liu, Z., W. G. Cumberland, L. E. Hultin, H. E. Prince, R. Detels, J. V. Giorgi. 1997. Elevated CD38 antigen expression on CD8+ T cells is a stronger marker for the risk of chronic HIV disease progression to AIDS and death in the Multicenter AIDS Cohort Study than CD4+ cell count, soluble immune activation markers, or combinations of HLA-DR and CD38 expression. J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 16: 83-92. [Medline]
  66. Choremi-Papadopoulou, H., V. Viglis, P. Gargalianos, T. Kordossis, A. Iniotaki-Theodoraki, J. Kosmidis. 1994. Downregulation of CD28 surface antigen on CD4+ and CD8+ T lymphocytes during HIV-1 infection. J. Acquir. Immune Defic. Syndr. 7: 245-253. [Medline]
  67. Rey-Cuille, M. A., J. L. Berthier, M. C. Bomsel-Demontoy, Y. Chaduc, L. Montagnier, A. G. Hovanessian, L. A. Chakrabarti. 1998. Simian immunodeficiency virus replicates to high levels in sooty mangabeys without inducing disease. J. Virol. 72: 3872-3886. [Abstract/Free Full Text]
  68. Chakrabarti, L. A., S. R. Lewin, L. Zhang, A. Gettie, A. Luckay, L. N. Martin, E. Skulsky, D. D. Ho, C. Cheng-Mayer, P. A. Marx. 2000. Normal T-cell turnover in sooty mangabeys harboring active simian immunodeficiency virus infection. J. Virol. 74: 1209-1223. [Abstract/Free Full Text]
  69. Silvestri, G., D. L. Sodora, R. A. Koup, M. Paiardini, S. P. O’Neil, H. M. McClure, S. I. Staprans, M. B. Feinberg. 2003. Nonpathogenic SIV infection of sooty mangabeys is characterized by limited bystander immunopathology despite chronic high-level viremia. Immunity 18: 441-452. [Medline]
  70. Silvestri, G., A. Fedanov, S. Germon, N. Kozyr, W. J. Kaiser, D. A. Garber, H. McClure, M. B. Feinberg, S. I. Staprans. 2005. Divergent host responses during primary simian immunodeficiency virus SIVsm infection of natural sooty mangabey and nonnatural rhesus macaque hosts. J. Virol. 79: 4043-4054. [Abstract/Free Full Text]
  71. Sumpter, B., R. Dunham, S. Gordon, J. Engram, M. Hennessy, A. Kinter, M. Paiardini, B. Cervasi, N. Klatt, H. McClure, et al 2007. Correlates of preserved CD4+ T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J. Immunol. 178: 1680-1691. [Abstract/Free Full Text]
  72. Silvestri, G.. 2005. Naturally SIV-infected sooty mangabeys: are we closer to understanding why they do not develop AIDS?. J. Med. Primatol. 34: 243-252. [Medline]
  73. Fukada, K., Y. Sobao, H. Tomiyama, S. Oka, M. Takiguchi. 2002. Functional expression of the chemokine receptor CCR5 on virus epitope-specific memory and effector CD8+ T cells. J. Immunol. 168: 2225-2232. [Abstract/Free Full Text]
  74. Stevenson, M.. 2003. HIV-1 pathogenesis. Nat. Med. 9: 853-860. [Medline]
  75. Forster, R., G. Schweigard, S. Johann, T. Emrich, E. Kremmer, C. Nerl, M. Lipp. 1997. Abnormal expression of the B-cell homing chemokine receptor BLR1 during the progression of acquired immunodeficiency syndrome. Blood 90: 520-525. [Abstract/Free Full Text]
  76. De Milito, A.. 2004. B lymphocyte dysfunctions in HIV infection. Curr. HIV Res. 2: 11-21. [Medline]
  77. LaBonte, M. L., P. F. McKay, N. L. Letvin. 2006. Evidence of NK cell dysfunction in SIV-infected rhesus monkeys: impairment of cytokine secretion and NKG2C/C2 expression. Eur. J. Immunol. 36: 2424-2433. [Medline]
  78. Weissman, D., A. S. Fauci. 1997. Role of dendritic cells in immunopathogenesis of human immunodeficiency virus infection. Clinical Microbiol. Rev. 10: 358-367. [Abstract]
  79. Gaines, H., M. A. von Sydow, L. V. von Stedingk, G. Biberfeld, B. Bottiger, L. O. Hansson, P. Lundbergh, A. B. Sonnerborg, J. Wasserman, O. O. Strannegaard. 1990. Immunological changes in primary HIV-1 infection. AIDS 4: 995-999. [Medline]
  80. Roederer, M., J. G. Dubs, M. T. Anderson, P. A. Raju, L. A. Herzenberg, L. A. Herzenberg. 1995. CD8 naive T cell counts decrease progressively in HIV-infected adults. J. Clin. Invest. 95: 2061-2066. [Medline]
  81. Gougeon, M. L.. 2003. Apoptosis as an HIV strategy to escape immune attack. Nat. Rev. Immunol. 3: 392-404. [Medline]
  82. Badley, A. D., A. A. Pilon, A. Landay, D. H. Lynch. 2000. Mechanisms of HIV-associated lymphocyte apoptosis. Blood 96: 2951-2964. [Abstract/Free Full Text]
  83. Fluur, C., A. De Milito, T. J. Fry, N. Vivar, L. Eidsmo, A. Atlas, C. Federici, P. Matarrese, M. Logozzi, E. Rajnavolgyi, et al 2007. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection. J. Immunol. 178: 5340-5350. [Abstract/Free Full Text]
  84. Golden-Mason, L., J. R. Burton, Jr, N. Castelblanco, J. Klarquist, S. Benlloch, C. Wang, H. R. Rosen. 2006. Loss of IL-7 receptor alpha-chain (CD127) expression in acute HCV infection associated with viral persistence. Hepatology 44: 1098-1109. [Medline]
  85. Fry, T. J., C. L. Mackall. 2002. Interleukin-7: from bench to clinic. Blood 99: 3892-3904. [Free Full Text]
  86. Moniuszko, M., T. Fry, W. P. Tsai, M. Morre, B. Assouline, P. Cortez, M. G. Lewis, S. Cairns, C. Mackall, G. Franchini. 2004. Recombinant interleukin-7 induces proliferation of naive macaque CD4+ and CD8+ T cells in vivo. J. Virol. 78: 9740-9749. [Abstract/Free Full Text]
  87. Beq, S., M. T. Nugeyre, R. Ho Tsong Fang, D. Gautier, R. Legrand, N. Schmitt, J. Estaquier, F. Barre-Sinoussi, B. Hurtrel, R. Cheynier, N. Israel. 2006. IL-7 induces immunological improvement in SIV-infected rhesus macaques under antiviral therapy. J. Immunol. 176: 914-922. [Abstract/Free Full Text]
  88. Fry, T. J., M. Moniuszko, S. Creekmore, S. J. Donohue, D. C. Douek, S. Giardina, T. T. Hecht, B. J. Hill, K. Komschlies, J. Tomaszewski, et al 2003. IL-7 therapy dramatically alters peripheral T-cell homeostasis in normal and SIV-infected nonhuman primates. Blood 101: 2294-2299. [Abstract/Free Full Text]
  89. Nugeyre, M. T., V. Monceaux, S. Beq, M. C. Cumont, R. Ho Tsong Fang, L. Chene, M. Morre, F. Barre-Sinoussi, B. Hurtrel, N. Israel. 2003. IL-7 stimulates T cell renewal without increasing viral replication in simian immunodeficiency virus-infected macaques. J. Immunol. 171: 4447-4453. [Abstract/Free Full Text]
  90. Rosenberg, S. A., C. Sportes, M. Ahmadzadeh, T. J. Fry, L. T. Ngo, S. L. Schwarz, M. Stetler-Stevenson, K. E. Morton, S. A. Mavroukakis, M. Morre, et al 2006. IL-7 administration to humans leads to expansion of CD8+ and CD4+ cells but a relative decrease of CD4+ T-regulatory cells. J. Immunother. 29: 313-319. [Medline]



This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Dabrowska, N. Kim, and A. Aldovini
Tat-Induced FOXO3a Is a Key Mediator of Apoptosis in HIV-1-Infected Human CD4+ T Lymphocytes
J. Immunol., December 15, 2008; 181(12): 8460 - 8477.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dunham, R. M.
Right arrow Articles by Silvestri, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunham, R. M.
Right arrow Articles by Silvestri, G.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS