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The Journal of Immunology, 2008, 180, 4754 -4762
Copyright © 2008 by The American Association of Immunologists, Inc.
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*Lung Transplantation

CD4+ T Lymphocytes Are Not Necessary for the Acute Rejection of Vascularized Mouse Lung Transplants1

Andrew E. Gelman*, Mikio Okazaki*, Jiaming Lai*, Christopher G. Kornfeld*, Friederike H. Kreisel{dagger}, Steven B. Richardson*, Seiichiro Sugimoto*, Jeremy R. Tietjens*, G. Alexander Patterson*, Alexander S. Krupnick* and Daniel Kreisel2,*

* Division of Cardiothoracic Surgery, Department of Surgery, and {dagger} Department of Pathology and Immunology, Washington University, St. Louis, MO 63110


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Acute rejection continues to present a major obstacle to successful lung transplantation. Although CD4+ T lymphocytes are critical for the rejection of some solid organ grafts, the role of CD4+ T cells in the rejection of lung allografts is largely unknown. In this study, we demonstrate in a novel model of orthotopic vascularized mouse lung transplantation that acute rejection of lung allografts is independent of CD4+ T cell-mediated allorecognition pathways. CD4+ T cell-independent rejection occurs in the absence of donor-derived graft-resident hematopoietic APCs. Furthermore, blockade of the CD28/B7 costimulatory pathways attenuates acute lung allograft rejection in the absence of CD4+ T cells, but does not delay acute rejection when CD4+ T cells are present. Our results provide new mechanistic insight into the acute rejection of lung allografts and highlight the importance of identifying differences in pathways that regulate the rejection of various organs.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Although it is well established that T lymphocytes play a critical role in mediating allograft rejection, it is unclear how CD4+ and CD8+ T cells differentially regulate this immune process. The vast majority of studies in mice have suggested that CD4+ T lymphocytes are both necessary and sufficient for the acute rejection of cardiac allografts (1, 2). To this end, CD4+ T cell depletion can lead to long-term survival of cardiac allografts, indicating that CD8+ T cells alone cannot mediate this rejection (1). Moreover, we and others have shown that activation of the CD4+ T cells is dependent on the direct allorecognition pathway through graft-resident hematopoietic cells (3, 4). Analogous to heart allograft rejection, most reports indicate that the rejection of pancreatic islet allografts by unprimed recipients requires CD4+ T cells (5, 6, 7, 8). Similarly, earlier studies have indicated that the rejection of corneal allografts is dependent on CD4+ T cells (9). However, this notion was challenged by newer reports that show that the rejection of allogeneic corneas can be mediated in the absence of CD4+ T cells (10). Although it is well established that CD4+ T cells can independently reject skin allografts, numerous papers indicate that CD8+ T cells can also reject allogeneic skin grafts in the absence of CD4+ T cell help (11, 12, 13, 14). Both CD4+ and CD8+ T cells can independently reject intestinal allografts and allogeneic hepatocytes in mice (15, 16, 17, 18, 19). Taken together, these studies suggest that the characteristics of the specific allograft tissue may influence the relative contribution of CD4+ and CD8+ T cells to acute allograft rejection.

Importantly, there are differences between various organs regarding their susceptibility to rejection. Skin and small bowel appear to be most susceptible to rejection in mice, whereas pancreatic islets, hearts, kidneys, and livers are progressively more easily accepted (20). There is also evidence that CD4+ and CD8+ T cell-mediated rejection responses against allografts may depend on different costimulatory signals and have different susceptibilities to immunosuppression. It is therefore critical to define the relative contributions of CD4+ and CD8+ T cells in mediating allograft rejection in specific organs (12, 21, 22, 23).

Similar to mice, organs differ with regard to their immunogenicity in humans as well. To this end, outcomes after lung transplantation in humans are far worse than those after transplantation of other solid organs (24). The 5-year survival is <50% and the majority of patients succumb to obliterative bronchiolitis. This condition is characterized by progressive fibrous obliteration of the distal airways and is thought to be a manifestation of chronic rejection. The frequency and severity of acute rejection have been shown to be a risk factor for the development of chronic rejection (25). Interestingly, a single episode of acute rejection was found to be a predictor of bronchiolitis obliterans independent of other risk factors (26). Mechanisms that lead to both acute and chronic lung allograft rejection are poorly understood because their study has been hampered by the lack of a physiologically relevant mouse model of lung transplantation. To date, the most frequently used model to study immunological pathways leading to lung allograft rejection has been the heterotopic transplantation of tracheal allografts. Although both CD4+ and CD8+ T cells have been shown to contribute to obliteration of the airway, this model only employs the proximal airway and is not vascularized. Therefore, it may not be representative of the pathophysiology of lung allografts (27, 28). We have recently developed a novel model of orthotopic vascularized lung transplantation in the mouse and have described marked differences between outcomes of orthotopic vascularized mouse lung transplants and heterotopic tracheal transplants (29, 30).

In this study, we examined the role of CD4+ T cells in the acute rejection of vascularized lung allografts. We show that lung allografts are acutely rejected in the absence of CD4+ T cells. Similar to our previous findings in a heart transplant model, triggering of CD4+ T cell-independent rejection can occur in the absence of donor-derived graft-resident hematopoietic cells (29). Moreover, blockade of the CD28/B7 costimulatory pathway delays lung allograft rejection in the absence of CD4+ T cells, but does not alter the tempo of acute rejection when CD4+ T cells are present.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Disclosures
 References
 
Animals

Male inbred C57BL/6 (H-2Kb) (designated as B6), CD4-knockout B6 (B6.129S2-Cd4tm1Mak/J), BALB/c (H-2Kd), and CBA/Ca (H-2Kk) (designated as CBA) mice were purchased from The Jackson Laboratory. Male MHC class II-deficient B6 mice (B6.129-H2-Ab1tm1GruN12) were purchased from Taconic Farms. Ten- to 12-wk-old animals weighing 25–30 g were used as both donors and recipients.

Bone marrow transplantation

Bone marrow chimeras were created, as previously described (31). Briefly, for bone marrow transplantation, male wild-type B6 were reconstituted with marrow from CBA mice. Bone marrow was harvested from the femora of donor mice, and T lymphocytes were depleted with anti-CD90 (Thy)-labeled magnetic microbeads (Miltenyi Biotec). Recipient mice received an inoculum of 1 x 107 T cell-depleted donor bone marrow via lateral tail vein injection 6 h after lethal irradiation (10 Gy). These bone marrow chimeras were used as lung donors at least 90 days after the bone marrow transplant. The following designation is used to describe chimeric organs: nonhematopoietic cells (hematopoietic APCs).

Replacement of lung-resident hematopoietic APCs in bone marrow chimeras was assessed by flow cytometry. Lung tissue derived from wild-type B6 and CBA mice as well as B6(CBA) bone marrow chimeras was cut into 2-mm slices and digested by placing them into a RPMI 1640 solution containing type 2 collagenase (0.5 mg/ml; Worthington Biochemical) and 5 U/ml DNase (Sigma-Aldrich) for 90 min. The digested lung tissue was then passed through a 70-µm cell strainer and treated with ACK lysing buffer. Replacement of hematopoietic APCs was evaluated by staining with fluorochrome-labeled anti-CD45 (clone 30-F11), anti-I-Ab (clone 25-9-17), anti-I-Ak (clone 11-5.2), and respective isotype control Abs (BD Biosciences).

Lung transplantation

Left orthotopic vascularized lung transplants were performed using cuff techniques, as previously described (29). In some experimental groups, CD4+ T lymphocytes were depleted, as previously described (GK1.5 100 µg i.p. at days –2 and –1) (BIOExpress), and depletion was maintained with weekly injections (31). Some animals were treated with CTLA4Ig (200 µg i.p.) on day 2 or days 0, 2, 4, and 6 (BIOExpress). Control Ig was purchased from Jackson ImmunoResearch Laboratories.

Histology

Portions of the transplanted lungs were fixed in formaldehyde, sectioned, and stained with H&E. Grading for acute cellular rejection was performed in a blinded fashion by a pathologist (F. Kreisel) using standard criteria developed by the Lung Rejection Study Group (32). The grading scale was as follows: grade A0, no cellular infiltrates; grade A1, minimal acute rejection with perivascular infiltrates comprising only one or two layers of predominantly small lymphocytes; grade A2, mild acute rejection with several layers of small and large lymphoid cells cuffing vessels and endothelialitis; grade A3, moderate acute rejection with spread of infiltrates into the interstitium of alveolar septae and spaces as well as predominance of large lymphoid cells and intra-alveolar accumulation of macrophages and neutrophils; grade A4, severe acute rejection characterized by vascular thrombosis with massive intra-alveolar hemorrhage and infarction, resulting in diffuse alveolar damage with necrosis of alveolar epithelium.

Flow cytometry

Seven days after transplantation, portions of transplanted lungs were prepared for flow cytometric analysis. Cellular infiltration into lungs was assessed by staining with fluorochrome-labeled anti-CD90.2 (clone 30-H12), anti-CD4 (clone RM4-5), anti-CD8 (clone 53-6.7), anti-NK1.1 (clone PK136), anti-{gamma}{delta} TCR (clone GL3), anti-CD25 (clone PC61), anti-CD62 ligand (clone MEL-14), and anti-CD44 (clone IM7) Abs as well as their respective isotype controls (BD Biosciences and eBioscience). Intracellular staining was performed with anti-IFN-{gamma} (clone XMG1.2), anti-IL-4 (clone 11B11; BD Pharmingen), anti-Foxp3 (clone FJK-16s; eBioscience), and their respective isotype controls. For intracellular cytokine staining of graft-infiltrating T lymphocytes, cells were cultured after lung digestion with 20 ng/ml PMA (Sigma-Aldrich) and 1 µM ionomycin (Calbiochem) for 4 h. Monensin (2 µM; Sigma-Aldrich) was added for the last 3 h of culture. Cells were subsequently surface stained for 30 min in a small volume of cold PBS, followed by a 60-min period of fixation in 2.5% paraformaldehyde (Sigma-Aldrich) on ice. Cells were then permeabilized with 0.1% saponin (Sigma-Aldrich) in PBS containing 2% FCS for 10 min on ice. Intracellular staining was performed for 30 min with the cells resuspended in a small volume of 0.1% saponin. After two washes in 0.01% saponin, the cells were resuspended in PBS containing 2% FCS and analyzed by flow cytometry. For absolute live cell counts, 50,000 CD45.1+ splenocytes labeled with fluorochrome-labeled anti-CD45.1 mAb (A20; BD Pharmingen) were also added to stained T cell sample FACS tubes just before FACS analysis. Live CD4+ or CD8+ T cell counts were calculated by taking the ratio of the number of CD4+ or CD8+ CD45.1– live events collected, respectively, to the number of CD45.1+ events collected and multiplying by 50,000.

Statistical analysis

Histological scores, numbers of graft-infiltrating T lymphocytes, and intracellular IFN-{gamma} levels are expressed as the mean ± SEM. Statistical analysis to assess significant differences between experimental groups was performed by using Student’s t test. Results were considered statistically different if p < 0.05.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Disclosures
 References
 
Graft-infiltrating CD8+ T cells predominate over CD4+ T cells in rejected mouse lung allografts

We have previously shown that, similar to the rejection of cardiac allografts in this strain combination, B6 lungs show histological evidence of acute vascular rejection 7 days after transplantation into CBA recipients (29). We first wanted to assess the absolute numbers of graft-infiltrating CD4+ and CD8+ T cells in vascularized lung transplants at the time of acute allograft rejection. For this purpose, we performed orthotopic vascularized lung transplants in the syngeneic B6->B6 and allogeneic B6->CBA strain combinations. The absolute number of both graft-infiltrating CD4+ and CD8+ T cells was significantly lower in syngeneic grafts than allogeneic grafts (Fig. 1A). The ratio of CD8+ to CD4+ T cells was 0.9 ± 0.2 in syngeneic grafts. Interestingly, in allogeneic grafts there were twice as many CD8+ T cells than CD4+ T cells with a ratio of 1.9 ± 0.3. We further evaluated graft-infiltrating CD4+ and CD8+ T cells in allogeneic transplants (Fig. 1B). Naive T cells express the CD62LhighCD44low phenotype, whereas the effector memory T cells express the CD62LlowCD44high phenotype (33). The majority of both CD4+ and CD8+ T cells within the B6->CBA lung grafts were CD62LlowCD44high, indicating an effector memory phenotype. Of note, a larger proportion of CD8+ T cells was CD62LlowCD44high when compared with CD4+ T cells. A small portion of graft-infiltrating Thy1.2 cells expressed neither CD4 nor CD8. Further analysis of this subset revealed that only a small percentage of these cells expressed NK1.1. Notably, up to half of these Thy1.2+CD4–CD8– cells expressed the {gamma}{delta}-TCR. Thus, our data indicate that, in contrast to other acutely rejected murine allografts, CD8+ T cells are more numerous than CD4+ T cells in acutely rejected mouse lung allografts (34).


Figure 1
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  		FIGURE 1. A, Absolute numbers of infiltrating CD4+ and CD8+ T cells in syngeneic (B6->B6) and allogeneic lung grafts (B6->CBA) 7 days following engraftment. T cell infiltrate is represented as mean absolute numbers ± SEM of intragraft CD4+ and CD8+ T cells (n = 4 per experimental group). Statistically significant differences between experimental groups are indicated and were calculated using Student’s t test. B, Characterization of live intragraft Thy1.2+ cells from B6->CBA lung grafts 7 days following transplantation. Results are representative of four independent experiments.

 
Mouse lung allografts are acutely rejected in the absence of CD4+ direct allorecognition

Having shown that CD8+ T lymphocytes are more predominant than CD4+ T lymphocytes in acutely rejected mouse lung allografts, we next set out to examine whether the acute rejection of mouse lung allografts was dependent on CD4+ T cells. To test the role of CD4+ T cell direct allorecognition in the acute rejection of vascularized mouse lung transplants, we transplanted B6 MHC class II-deficient (designated as B6II–) lungs into CBA hosts. Analogous to wild-type B6 lungs that were transplanted into CBA hosts, B6II– lungs demonstrated acute cellular rejection with perivascular cuffing and subepithelial lymphocytic infiltrates in the small airways 7 days after transplantation (Fig. 2A). Rejection scores were analogous for B6->CBA and B6II–->CBA lung grafts (Fig. 2B). We also analyzed cytokine expression in graft-infiltrating CD8+ T cells (Fig. 2C). The majority of graft-infiltrating CD8+ T cells expressed IFN-{gamma} in both B6->CBA and B6II–->CBA combinations at comparable levels. We could not detect expression of IL-4 in graft-infiltrating CD8+ T cells, indicating a polarization toward the Tc1 phenotype (data not shown).


Figure 2
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  		FIGURE 2. Histological and flow cytometric analysis of B6->B6 (n = 3), B6->CBA (n = 4), and B6II–->CBA (n = 4) lung grafts 7 days after transplantation. A, H&E slides are represented at x400 magnification. B, Rejection scores represented as mean ± SEM. Statistical analysis was conducted with Student’s t test. C, Flow cytometric plots depict the percentage of live intragraft Thy1.2+ CD8+ cells that have the capacity to produce IFN-{gamma}. Mean percentage ± SEM of IFN-{gamma}-producing Thy1.2+ CD8+ cells were 23.9 ± 5.1 for B6->B6, 65.3 ± 5.5 for B6->CBA, and 67.6 ± 9.0 for B6II–->CBA lung transplants. Statistical analysis was conducted with Student’s t test; B6->CBA vs B6->B6, p < 0.05; B6II–->CBA vs B6->B6, p < 0.05; B6II–->CBA vs B6->CBA, p > 0.1.

 
CD4+ T cells are not necessary for the acute rejection of vascularized mouse lung allografts

We next set out to characterize the graft-infiltrating CD4+ T cells. Th1 and Th2 cells have been characterized as two classic effector CD4+ T cell subsets that secrete proinflammatory cytokines, such as IFN-{gamma}, or anti-inflammatory cytokines, such as IL-4, respectively. Recent studies have demonstrated that CD4+ T cells can also differentiate toward an independent lineage of IL-17-producing Th17 cells, which has been shown to play an important role in inflammatory responses (35). In addition, CD4+Foxp3+ T cells have been well described as a distinct lineage of regulatory CD4+ T cells. At 7 days after engraftment, approximately one-third of the graft-infiltrating CD4+ T cells in B6->CBA lung transplants express IFN-{gamma} consistent with a Th1 phenotype, and only very few CD4+ T cells express IL-17 (Fig. 3A). We were unable to detect any IL-4 expression in graft-infiltrating CD4+ T cells. Notably, ~25% of graft-infiltrating CD4+ T cells in acutely rejected B6->CBA lungs express Foxp3, indicating a regulatory phenotype. Of note, a large portion of the CD4+Foxp3+ T cells lacks expression of CD25 consistent with observations previously made for lung-resident regulatory CD4+ T cells (36). Thus, the population of graft-infiltrating CD4+ T cells is heterogeneous with presence of both IFN-{gamma}-expressing Th1 cells and Foxp3-expressing cells.


Figure 3
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  		FIGURE 3. Histological and flow cytometric analysis of B6->CD4+-depleted CBA/Ca lung grafts 7 days after transplantation (n = 4). A, Flow cytometric analysis of live Thy1.2+ CD4+ T cells in B6->CBA lungs transplants 7 days after engraftment. Plots are representative of three independent experiments. B, Intragraft live Thy1.2+ CD4+ cells in control Ig-treated (left) and GK1.5-treated (right) CBA recipients of B6 lungs 7 days after engraftment. C, H&E slides are represented at x400 magnification. D, Rejection score represented as a mean ± SEM recipient. Statistical analysis was conducted with Student’s t test; B6->CBA-CD4 vs B6->B6, p < 0.05; B6->CBA-CD4 vs B6->CBA, p > 0.1; B6->CBA-CD4 vs B6II–->CBA, p > 0.1. E, Flow cytometric plots depict the percentage of live intragraft Thy1.2+ CD8+ cells that have the capacity to produce IFN-{gamma}. Mean percentage ± SEM of IFN-{gamma}-producing Thy1.2+ CD8+ cells was 68.5 ± 4.1. Statistical analysis was conducted with Student’s t test; B6->CBA-CD4 vs B6->B6, p < 0.05; B6->CBA-CD4 vs B6->CBA, p > 0.1; B6->CBA-CD4 vs B6II–->CBA, p > 0.1. F, H&E slides of BALB/c->B6CD4+/+ (n = 4) and BALB/c->B6CD4–/– (n = 2) 7 days after transplantation. Rejection scores were A3 for all recipients in both experimental groups.

 
Having shown that the CD4+ T cell direct allorecognition pathway is not necessary for the acute rejection of lung allografts, we next wanted to determine whether CD4+ T cells are required to trigger acute rejection of lung allografts. For this purpose, we depleted CD4+ T cells in recipient CBA mice through the administration of a short course of preoperative CD4-specific Abs. We confirmed depletion of CD4+ T cells in the allografts by flow cytometrical analysis of rejected lungs (Fig. 3B). At 7 days after transplantation, these lung allografts showed acute cellular rejection with rejection scores analogous to B6->CBA and B6II–->CBA transplants (Fig. 3, C and D). Moreover, similar to what we observed in lung transplants in the B6->CBA and B6II–->CBA combinations, graft-infiltrating CD8+ T cells were polarized toward Tc1, with the majority of CD8+ T cells expressing IFN-{gamma} (Fig. 3E). There were no significant differences in the percentage of CD8+ T cells expressing IFN-{gamma} between B6->CBA, B6II–->CBA, and B6->CD4-depleted CBA recipients. Again, IL-4 was not detected in graft-infiltrating CD8+ T cells (data not shown). Next, we wanted to confirm these observations using an alternative approach. For this purpose, we transplanted BALB/c lungs into CD4 knockout B6 recipients (Fig. 3F). We have previously shown that BALB/c lungs are acutely rejected when transplanted into B6 recipients (30). Similarly, BALB/c lungs showed analogous acute rejection after transplantation into CD4 knockout B6 recipients. Taken together, these results show that the rejection of vascularized mouse lungs is independent of CD4+ T cells. Moreover, histological analysis revealed that the rejection scores were equivalent independent of the presence of CD4+ T cells (B6->CBA vs B6->CBA + GK1.5 and BALB/c->B6 vs BALB/c->B6 CD4 knockout), indicating that depletion of regulatory CD4+ T cells does not result in worsening of the acute rejection.

CD4+ T cell-independent acute rejection of lung allografts does not require graft-resident donor-derived hematopoietic APCs

We have previously shown that nonhematopoietic cells can activate alloreactive CD8+ T cells in a B7-dependent fashion in vitro and in vivo (37). Furthermore, our group has demonstrated in a TCR transgenic model that cardiac allografts can be rejected via CD8+ T cell direct allorecognition in the absence of Ag presentation by hematopoietic APCs (38). We next set out to determine whether CD4+ T cell-independent acute rejection of lung allografts was dependent on graft-resident donor-derived hematopoietic APCs. For this purpose, we used B6 bone marrow chimeras that had been reconstituted with CBA bone marrow as lung donors (designated as B6(CBA)). We have previously shown that bone marrow transplantation leads to near complete replacement of hematopoietic APCs such as dendritic cells and macrophages (31). We confirmed these findings in the B6(CBA) bone marrow chimeras (Fig. 4A). Although the lung-resident hematopoietic cells expressed I-Ak, we were unable to detect expression of I-Ab. These lungs were transplanted into CBA hosts that had been depleted of CD4+ T cells. At 7 days after engraftment, we observed histological signs of acute rejection (Fig. 4B). However, the rejection score was less severe when compared with lung transplants performed in the B6->CD4-depleted CBA strain combination, and the percentage of graft-infiltrating CD8+ T cells that expressed IFN-{gamma} in chimeric grafts was significantly lower when compared with B6 grafts (Fig. 4, C and D). We did not detect IL-4 in the graft-infiltrating CD8+ T cells at 7 days after engraftment (data not shown). At 14 days after engraftment into CD4-depleted CBA hosts, B6(CBA) grafts had histological evidence of worsening acute rejection with extensive perivascular cuffing and subepithelial lymphocytic infiltrates in the small airways, and there was a trend toward a higher percentage of graft-infiltrating CD8+ T cells expressing IFN-{gamma} (Fig. 4, B–D). Moreover, at 14 days after engraftment, the rejection scores of B6(CBA)->CD4-depleted CBA lungs were analogous to the ones observed in B6->CD4-depleted CBA mice at 7 days after transplantation (Fig. 4C). Moreover, there were no statistically significant differences between the percentage of IFN-{gamma}-producing CD8+ T cells in B6(CBA)->CD4-depleted CBA lungs at day 14 compared with in B6->CD4-depleted CBA at 7 days after transplantation (Fig. 4D). Thus, the activation of graft-infiltrating CD8+ T cells and the tempo of rejection are delayed in the absence of graft-resident donor-derived hematopoietic APCs. However, donor-derived hematopoietic APCs are not necessary for the CD4+ T cell-independent activation of CD8+ T cells and acute rejection of lung allografts.


Figure 4
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  		FIGURE 4. Histological and flow cytometric analysis of B6(CBA)->CD4+ T cell-depleted CBA 7 days (n = 4) and 14 days (n = 3) after transplantation. A, Flow cytometric analysis of I-Ab and I-Ak expression on lung-resident live CD45+ cells in B6, CBA, and B6(CBA) mice. Shaded curves represent isotype controls. B, H&E slides are represented at x400 magnification. C, Rejection scores represented as mean ± SEM. Statistical analysis was conducted with Student’s t test; B6(CBA)->CBA-CD4 (day 7) vs B6->CBA-CD4 (day 7), p < 0.1; B6(CBA)->CBA-CD4 (day 7) vs B6(CBA)->CBA-CD4 (day 14), p < 0.1; B6(CBA)->CBA-CD4 (day 14) vs B6->CBA-CD4 (day 7), p > 0.1. D, Flow cytometric plots depict the percentage of live intragraft Thy1.2+ CD8+ cells that have the capacity to produce IFN-{gamma}. Mean percentage ± SEM of IFN-{gamma}-producing Thy1.2+ CD8+ cells were 55.9 ± 9.3 and 62.9 ± 8.9 for days 7 and 14, respectively. Statistical analysis was conducted with Student’s t test; B6(CBA)->CBA-CD4 (day 7) vs B6->CBA-CD4 (day 7), p < 0.05; B6(CBA)->CBA-CD4 (day 7) vs B6(CBA)->CBA-CD4 (day 14), p > 0.1; B6(CBA)->CBA-CD4 (day 14) vs B6-> CBA-CD4 (day 7), p > 0.1.

 
In the absence of CD4+ T cells, acute lung allograft rejection can be attenuated by CD28/B7 costimulatory blockade

Blockade of the B7:CD28 costimulatory pathway through treatment with a single dose of CTLA4Ig has been shown to lead to prolongation of survival of mouse cardiac allografts (39, 40). We next set out to investigate whether analogous treatment with a single dose of CTLA4Ig had any impact on the rejection response of lung allografts. To this end, CBA mice were treated with a single dose of CTLA4Ig on day 2 after transplantation of B6 lung allografts (Fig. 5A). Seven days after transplantation, these allografts were acutely rejected with perivascular cuffing and subepithelial lymphocytic infiltrates in the small airways. The rejection scores were analogous to those observed in untreated CBA recipients of B6 lung allografts (Fig. 5B). Analogous to our findings in B6 allografts that were transplanted into untreated CBA recipients, the majority of graft-infiltrating CD8+ T cells in B6 lung allografts of CTLA4Ig-treated CBA recipients expressed IFN-{gamma} (Fig. 5C). We also evaluated such grafts at 14 days after transplantation. Again, we observed severe acute rejection, and the majority of graft-infiltrating CD8+ T cells expressed IFN-{gamma} (Fig. 5, A–C). Thus, unlike the case for heart transplantation, a single dose of CTLA4Ig does not impact the tempo of lung allograft rejection.


Figure 5
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  		FIGURE 5. Histological and flow cytometric analysis of CTLA4Ig-treated B6->CBA 7 days (n = 3) and 14 days (n = 3) after transplantation as well as CTLA4Ig-treated B6->CD4+ T cell-depleted CBA 7 days (n = 5) and 14 days (n = 5) after transplantation. A, H&E slides are represented at x400 magnification. B, Rejection scores represented as mean ± SEM. Statistical analysis was conducted with Student’s t test; B6->CBA (day 7) vs B6->CBA + CTLA4Ig (day 7), p > 0.1. B6->CBA-CD4 (day 7) vs B6->CBA-CD4 + CTLA4Ig (day 7), p = 0.05. C, Flow cytometric plots depict the percentage of live intragraft Thy1.2+ CD8+ cells that have the capacity to produce IFN-{gamma}. In the case of CTLA4Ig-treated B6->CBA transplants, the mean percentage ± SEM of IFN-{gamma}-producing Thy1.2+ CD8+ cells were 66.1 ± 9.2 seven days after engraftment and 73.8 ± 1.9 fourteen days after engraftment. In the case of CD4-depleted CTLA4Ig-treated B6->CBA transplants, the mean percentage ± SEM of IFN-{gamma}-producing Thy1.2+ CD8+ cells were 38.2 ± 5.1 seven days after engraftment and 59.1 ± 5.7 fourteen days after engraftment. Statistical analysis was conducted with Student’s t test; B6->CBA-CD4 + CTLA4Ig (day 7) vs B6->CBA + CTLA4Ig (day 7), p < 0.005; B6->CBA-CD4 + CTLA4Ig (day 7) vs B6->CBA-CD4 + CTLA4Ig (day 14), p < 0.005; B6->CBA + CTLA4Ig (day 7) vs B6->CBA + CTLA4Ig (day 14), p > 0.1; B6->CBA-CD4 + CTLA4Ig (day 7) vs B6->CBA-CD4 (day 7), p < 0.001; B6->CBA vs B6->CBA + CTLA4Ig (day 7), p > 0.1. D, Histological analysis of B6->CD4-depleted CBA lung transplants that received CTLA4Ig (200 µg) on days 0, 2, 4, and 6. H&E slides are represented at x400 magnification. E, Rejection scores represented as mean ± SEM. Statistical analysis was conducted with Student’s t test; B6->CBA-CD4 + CTLA4Ig (days 0, 2, 4, and 6) (day 14) vs B6->CBA-CD4 + CTLA4Ig (day 2) (day 14), p > 0.1.

 
Having shown that blockade of the B7:CD28 costimulatory pathway through administration of a single dose of CTLA4Ig was not effective at preventing the activation of CD8+ T cells or lung allograft rejection in the presence of CD4+ T cells, we next wanted to examine whether this costimulatory blockade had any impact on the activation of CD8+ T cells or lung allograft rejection in the absence of CD4+ T cells. To this end, we treated CD4+ T cell-depleted CBA recipients of B6 lung allografts with a single dose of CTLA4Ig 2 days after transplantation. Compared with our observations with CTLA4Ig treatment in the presence of CD4+ T cells, we observed milder rejection in lung allografts 7 days after transplantation when CD4+ T cells were absent (Fig. 5, A and B). The percentage of graft-infiltrating CD8+ T cells that expressed IFN-{gamma} 7 days after transplantation was also significantly lower when compared with CTLA4Ig-treated wild-type recipients that had not received CD4-depleting Abs or CD4+ T cell-depleted animals that had not received costimulatory blockade (Fig. 5C). Previous reports have indicated that inhibition of allograft rejection by CTLA4Ig can be associated with a shift toward production of Th2 cytokines (41). We have not observed a shift toward Tc2 in the graft-infiltrating CD8+ T cells (data not shown). At 14 days after transplantation, however, lung allografts in CD4+ T cell-depleted CTLA4Ig-treated recipients had worsening rejection with severe perivascular lymphocytic cuffing and subepithelial mononuclear infiltrates in the airways (Fig. 5, A and B). In addition, a significantly larger percentage of graft-infiltrating CD8+ T cells expressed IFN-{gamma} at 14 days when compared with 7 days after transplantation (Fig. 5C). To evaluate whether earlier initiation and prolonged administration of CTLA4Ig enhance the protective effect and further attenuate the acute rejection, we treated CD4-depleted CBA recipients of B6 lungs with CTLA4Ig on days 0, 2, 4, and 6 (Fig. 5, D and E). The histological changes and rejection scores of these grafts at 14 days after transplantation were equivalent to those seen in animals that received a single dose of CTLA4-Ig on day 2. Thus, unlike our observations in the presence of CD4+ T cells, treatment with CTLA4Ig leads to an attenuation of the rejection response when CD4+ T cells are absent, but cannot prevent the ultimate rejection of the lung allografts.


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Disclosures
 References
 
Acute rejection continues to contribute to the morbidity of lung transplant recipients. In addition, acute rejection has also been demonstrated to be an important risk factor for the development of chronic lung allograft rejection (26). Elucidating mechanisms that lead to the rejection of lung allografts is of critical importance to improve outcomes after lung transplantation. However, immune pathways leading to the rejection of lung allografts are poorly understood, in part because such studies have been hampered by the lack of a physiological mouse model of lung transplantation. This study uses a novel mouse model of orthotopic vascularized lung transplantation and offers new mechanistic insight into in vivo mechanisms of lung allograft rejection. To our knowledge, this study shows for the first time that the acute rejection of lung allografts is not dependent on CD4+ T lymphocytes. Although strain-specific differences may exist with regard to cellular requirements for the acute rejection of vascularized lung allografts, CD8+ T cells seem to play a predominant role in the two allogeneic strain combinations used in this study. This report illustrates several important issues that have not been previously addressed.

The relative composition of graft-infiltrating T lymphocytes varies considerably between solid organ grafts. Clinical studies have shown that CD4+ T cells are more numerous than CD8+ T cells in heart allografts undergoing acute rejection (42). Studies analyzing graft-infiltrating lymphocytes in acutely rejected mouse cardiac allografts as well as pancreatic islet allografts have shown equivalent percentages of CD4+ and CD8+ T cells (34, 43, 44). Although CD8+ T cells predominate over CD4+ T cells at early time points in heterotopically transplanted allogeneic murine tracheas, at later time points CD4+ T cells outnumber CD8+ T cells (45, 46). The composition of graft-infiltrating T lymphocytes in vascularized lung transplants had not been previously explored. We show that graft-infiltrating CD8+ T cells predominate over CD4+ T cells in acutely rejected mouse lung allografts. Interestingly, the analysis of bronchoalveolar lavage fluid from lung transplant recipients, who were diagnosed with acute rejection, has similarly demonstrated that CD8+ T cells were more abundant than CD4+ T cells (47, 48).

This predominance of CD8+ T cells over CD4+ T cells in acutely rejected mouse lung allografts led us to examine whether CD4+ T cells were necessary for the acute rejection of murine lungs. Previous studies have shown that transplantation of MHC class II-deficient cardiac allografts leads to long-term survival, demonstrating that CD4+ T cell-mediated direct allorecognition is critically important for the acute rejection of cardiac allografts (2, 49). In contrast to these findings, we observed that lung allografts were acutely rejected when the CD4+ direct allorecognition pathway was eliminated. Furthermore, whereas the majority of studies indicate that depletion of CD4+ T cells leads to prolonged or indefinite survival of allogeneic murine hearts and pancreatic islets, vascularized lung allografts are rejected in the absence of CD4+ T cells (1, 6, 8). CD4+ T cell-independent acute rejection has also been observed in the case of murine skin and intestinal allografts (11, 15). Our group has previously shown that graft-resident donor-derived hematopoietic APCs are not necessary to trigger CD4+ T cell-independent rejection of murine cardiac allografts (38). We have now extended these observations and show that lung allografts that carry recipient-type hematopoietic APCs are still capable of undergoing acute rejection when transplanted into a CD4+ T cell-depleted host, albeit at a delayed tempo when compared with lung allografts with graft-resident donor-derived hematopoietic APCs. Although direct CD8+ allorecognition by hematopoietic APCs is virtually eliminated after transplantation of lungs from bone marrow chimeras, radioresistant nonhematopoietic graft cells are not replaced following bone marrow transplantation. We and others have previously demonstrated that nonhematopoietic cells such as vascular endothelial cells can activate allogeneic CD8+ T cells in vitro and in vivo (37, 38, 50). Notably, compared with other grafts, lungs are rich in nonhematopoietic cells because, in addition to vascular endothelium, airway epithelial cells, a population of cells unique to lung allografts, also express MHC class I (51). Thus, the presentation of allogeneic MHC class I molecules by nonhematopoietic graft cells may play an important role in the activation of recipient CD8+ T cells after lung transplantation. CD8+ T lymphocytes can also recognize exogenous Ag presented in the context of self-MHC class I molecules (52). However, recent studies have indicated that indirectly primed CD8+ T cells do not play a major role in shaping immune responses to allografts (53).

Although a single dose of CTLA4Ig has been shown to lead to prolonged survival of mouse cardiac allografts, this regimen does not alter the rejection tempo of mouse lung transplants when CD4+ T cells are present (39, 40). Of note, similar to our observation in lung transplants, blockade of the B7:CD28 costimulatory pathway does not lead to prolongation of survival of skin allografts or small bowel transplants in wild-type murine recipients (15, 54). In the absence of CD4+ T cells, however, blockade of the B7:CD28 costimulatory pathway delays the tempo of rejection, suggesting that CD4+ T cells contribute to acute rejection in CTLA4Ig-treated animals. These observations are supported by previous reports describing the dependence of CD8+ T cell activation on B7/28 costimulation in the absence of CD4+ T cells (55, 56). Furthermore, we have previously shown that nonhematopoietic cells such as vascular endothelial cells can activate alloreactive CD8+ T cells in B7-dependent fashion both in vitro and in vivo (37). Various tissue and solid organ grafts differ with regard to their quantity and composition of nonhematopoietic cells. Compared with other grafts such as skin and hepatocytes, lungs are rich in nonhematopoietic cells such as vascular endothelial cells and airway epithelial cells. Interestingly, bronchial epithelial cells, a nonhematopoietic cell population unique to lung grafts, have been shown to express B7 after lung transplantation in humans (57). Thus, attenuated rejection after CD28/B7 blockade in CD4-depleted lung transplant recipients may at least in part be due to inhibition of CD8+ T cell activation by nonhematopoietic allograft cells.

This is the first study that examines the T cell-dependent mechanisms of acute rejection in vascularized mouse lung allografts. We demonstrate that the acute rejection of lung allografts is independent of CD4+ T cells, which is in contrast to observations made in other cellular and solid organ allografts. Our observations extend previous findings that the mechanisms that control rejection vary between transplanted organs (20). Therefore, lessons learned from the rejection of one specific organ cannot be necessarily applied to other organs, and our findings indicate that CD4+ T cell depletion strategies alone are not suitable to achieve tolerance after lung transplantation. In conclusion, this study provides new mechanistic insight into the acute rejection of lung allografts, which should help support investigative efforts to design strategies to improve short- and long-term outcomes following lung transplantation.


   Disclosures
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 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
References
 
The authors have no financial conflict of interest.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 A.E.G. is supported by a grant from The National Heart, Lung, and Blood Institute (1R43HL87456-01). D.K. is supported by a grant jointly sponsored by The National Heart, Lung, and Blood Institute and The Thoracic Surgery Foundation for Research and Education (1K08HL083983-01), by the American Association for Thoracic Surgery John W. Kirklin Research Scholarship, and by a grant from the Roche Organ Transplantation Research Foundation. Back

2 Address correspondence and reprint requests to Dr. Daniel Kreisel, Assistant Professor of Surgery, Pathology & Immunology, Campus Box 8234, 660 South Euclid Avenue, Washington University, St. Louis, MO 63110-1013. E-mail address: kreiseld{at}wudosis.wustl.edu Back

Received for publication June 14, 2007. Accepted for publication February 3, 2008.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 

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