The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 2008, 180, 2007
Copyright © 2008 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poulopoulou, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poulopoulou, C.

Comment on "TCR Activation Eliminates Glutamate Receptor GluR3 from the Cell Surface of Normal Human T Cells, via an Autocrine/Paracrine Granzyme B-Mediated Proteolytic Cleavage"

Cornelia Poulopoulou

Medical School of Athens Department of Neurology Experimental Neurophysiology Laboratory Athens, Greece

Being strongly interested in the role of glutamate in the immune system and more specifically on its effects on lymphocyte physiology, we read the paper by Ganor et al. (1) with great interest. We would like to complement the authors for the clear and, for the most part, very well documented data. However, a potentially critical question was raised that we would like to bring to the authors’ kind attention and ask for clarification. The question concerns the use of RPMI 1640 as a culture medium in experiments where the effects of 10 nM glutamate were studied. The authors test the ability of 10 nM glutamate to induce T cell adhesion to laminin while cells are bathed in RPMI 1640, a medium with a glutamate concentration of >100 µM (20 mg/L). Could the authors explain how they voided glutamate from the medium and thus were able to evaluate the effects of such a low concentration of glutamate?

References

  1. Ganor, Y., V. I. Teichberg, M. Levite. 2007. TCR activation eliminates glutamate receptor GluR3 from the cell surface of normal human T cells, via an autocrine/paracrine granzyme B-mediated proteolytic cleavage. J. Immunol. 178: 683-692. [Abstract/Free Full Text]



This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. Bombardieri, F. Barone, and C. Pitzalis
Response to Comment on "Activation-Induced Cytidine Deaminase Expression in Follicular Dendritic Cell Networks and Interfollicular Large B Cells Supports Functionality of Ectopic Lymphoid Neogenesis in Autoimmune Sialoadenitis and MALT Lymphoma in Sjogren's Syndrome"
J. Immunol., February 15, 2008; 180(4): 2008 - 2009.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Levite
Response to Comment on "TCR Activation Eliminates Glutamate Receptor GluR3 from the Cell Surface of Normal Human T Cells, via an Autocrine/Paracrine Granzyme B-Mediated Proteolytic Cleavage"
J. Immunol., February 15, 2008; 180(4): 2007 - 2007.
[Full Text] [PDF]

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poulopoulou, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poulopoulou, C.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS