Immunotherapy with CpG Oligonucleotides and Antibodies to TNF- Rescues Neonatal Mice from Lethal Arenavirus-Induced Meningoencephalitis

Data Supplement

Supplemental Videos. Anti-TNF£\ treatment reverses Tacaribe-induced paralysis. TCRV infected C57BL/6 mice were left untreated until signs of paralysis were evident on day 13 p.i. Mice exhibiting paralysis of 2 or more limbs usually die within 18 h. Video 1 shows the mouse prior to treatment. On day 13 p. i., the mouse was treated with hamster anti-mouse TNFƒÑ mAb (2E2). Videos 2 and 3 show the same mouse two and five days after treatment (days 15 and 18 p. i. respectively). Note that in video 2 the left hind-limb is paralysed, while in video 3, although movement is still slightly impaired neither hind limb is paralysed. Shown is one of multiple experiments with similar results.

Files in this Data Supplement:

• Video 1 (MOV, 3.13 Mb)
  - Anti-TNFa treatment reverses Tacaribe-induced paralysis. TCRV infected C57BL/6 mice were left untreated until signs of paralysis were evident on day 13 p.i. Mice exhibiting paralysis of 2 or more limbs usually die within 18 h. Video 1 shows the mouse prior to treatment.
• Video 2 (MOV, 1.72 Mb)
  - On day 13 p. i., the mouse was treated with hamster anti-mouse TNFa mAb (2E2). Videos 2 and 3 show the same mouse two and five days after treatment (days 15 and 18 p. i. respectively). Note that in video 2 the left hind-limb is paralysed, while in video 3, although movement is still slightly impaired neither hind limb is paralysed. Shown is one of multiple experiments with similar results.
• Video 3 (MOV, 3.81 Mb)